Seeking a challenging position in a prestigious research organization with an opportunity to make positive contributions while utilizing extensive experience, knowledge and expertise in onco-immunology and molecular biology Research scientist with 12 years of extensive laboratory experience and core skills in cellular and molecular biology, immunology and tissue culture. Strong trouble-shooting and multi-tasking skills and ability to collaborate on multiple assignments with proven background in experimental design, execution, data analysis and interpretation. Demonstrated ability to execute projects independently as well as in a team environment under strict deadlines with protocol adherence. Technical Skills Molecular and Cellular Biology: DNA and RNA isolation from cultured cells and various tissue specimens, nucleic acid extractions and quantifications, laser capture microdissection studies, genotyping, PCR, real-time PCR, hybridization, DNA sequencing analysis, plasmid preparation, gel electrophoresis Immunology and Biochemistry: ELISA, Meso Scale Discovery assays, flow cytometry, cellular cytotoxicity, intracellular cytokine staining, cell proliferation, immune cell isolations, immunoprecipitation, protein staining, kinetic studies, protein densitometry, western blot analysis, enzymatic assays, protein/antibody labeling, lipoprint analysis Immunohistochemistry: Broad exposure to numerous antibody staining techniques, different histology staining techniques like H&E, mast cell staining, trichrome, etc, fluorescent and light microscopy, photomicrography and quantitative image analysis utilizing Image-Pro Plus software In vitro: Hands-on experience in various cell lines, mammalian cell culture, human primary cell lines, transient and stable cell line generation, cell viability and proliferation assays, clonogenic assays In vivo: High-proficiency with small animal surgical procedures, imaging techniques, subcutaneous injections, intravenous injections, expression of biomarkers using real-time imaging, genotyping, tissue harvesting
Project Management, Data Management and Analysis, Developing SOPs and GLP, Writing Technical Reports Prism, SigmaPlot, PubMed, Reference Manager, Image-Pro Plus, Microsoft Office, Photoshop
November 2013 to Current
Company NameCity, StateResearch Associate
Coordinated and executed in vitro assays and developed ex vivo studies with emphasis on immunology, oncology, metabolic and neurodegenerative disease Specific Responsibilities: In vivo and in vitro studies for validation of potential drug development targets and to characterize pharmacological compounds Developed protocol to isolate and characterize mouse bone marrow derived dendritic cells Optimized mouse mixed lymphocyte reactions and binding assays and performed cell separations with MACS columns Successfully performed sample preparations (single-cell suspensions), cell separation (T- cell, B-cell) and cell analysis using FACS Investigated stimulating effects of lipopolysaccharides (LPS) on proliferative kinetics of B-cells Designed and studied cytokine-driven proliferation of T-cells Successfully developed cytotoxic T lymphocyte (CTL) assay Optimized cytokine Meso Scale Discovery (MSD) immunoassay studies In vitro assays utilizing mammalian cell culture and aseptic techniques Performed and optimized RNA isolation from blood and different organs and profiled the quality and quantity of RNA samples using Agilent Bioanalyzer and Nanodrop systems Performed Taqman gene expression assays on different targets to study fibrosis Successfully designed and developed Southern blot analysis on different mouse tissues using genomic DNA for various knockin and knockout lines Designed and established biochemical ex vivo models to study angiogenesis Designed and optimized lipoprotein fraction and sub-fraction studies using Lipoprint for target evaluation Optimized hematology profile studies to characterize drug effects Conducted quantitative metastatic and spontaneous tumor studies by in vivo bioluminescent imaging technique Performed ELISAs, EIAs and RIAs to measure insulin, leptin, adiponectin, aldosterone and corticosterone in mouse and rat plasma as affected by treatment.
Independently conducts experiments and analyzes data; suggests and implements modifications to the experimental procedures and data analysis.
January 2004 to November 2013
Company NameCity, StateResearch Assistant
Worked in radiation biology research with the primary focus on investigation of mechanisms of normal tissue radiation injury using radiation induced fibrosis and development of strategies to lessen/prevent these injuries Specific responsibilities: Planned, designed and executed various experiments from protocol development to data generation, analysis and interpretation while ensuring reproducible results Successfully utilized molecular and cellular biology procedures on a daily basis, including ELISA technique, Western blotting, immunoprecipitation, enzymatic assays, nucleic acid isolations, cDNA synthesis, LCM, protein extractions and quantitations, spectroscopy and kinetic assays Strong knowledge and extensive experience with different stainings, immunohistochemistry techniques, image processing, analysis and quantification of stained sections using ImagePro Plus software Proficiency in light and confocal microscopy studies Conducted laser capture microdissection (LCM) for tissue-specific gene expression analysis Independently conducted various experiments on endothelial cells for compound screenings Hands-on in vivo experience in blood collection, tissue harvesting, tail snipping and genotyping Compiled and analyzed experimental and performance data; developed accurate reports that offer and support critical decision-making Provided extensive training of molecular biology, protein immunoassays, immunostainings and cell culture techniques for research staff / colleagues Demonstrated better practice sharing by supporting other researchers in conducting in vivo and in vitro research experiments Accurate documentation of experimental work Promoted laboratory safety and day to day operational efficiency through proactive maintenance and adherence to safety regulations Key accomplishments include: Successfully optimized and implemented a wide range of nucleic acid amplifications and detections using quantitative PCR (qPCR) techniques for various gene expression levels Standardized different staining methods for histology studies Carried out laser capture microdissection on intestinal smooth muscle layer for different biomarker expressions Optimized protocol for expression and quantification of target biomarkers using real time in vivo imaging system (Vevo2100 system) Successfully labeled antibodies with biotin for endogenous expression and quantification of different proteins using in vivo imaging techniques Designed, streamlined, implemented and monitored standard operating procedures (SOPs).
January 1999 to January 2001
Company NameCityResearch Associate Volunteer
Participated in various research projects including study of biochemical parameters, effect of pollutants on metabolic activities, expression of heat shock proteins.
Assisted with reviewing, interpreting and summarizing data findings and participated in scientific writings and technical report preparations.
Institute of Science City, IndiaZoology and Biotechnology Master of ScienceZoology and Biotechnology
Pune University City, IndiaBiological Sciences Bachelor of ScienceBiological Sciences
Berbée M, Wang J, Kulkarni A and Hauer-Jensen M, "Increased radiation-induced intestinal injury after localized irradiation in spontaneously hypertensive rats". Obstetrics and Gynecology, submitted (under review). Wang J, Zheng J, Kulkarni A, Wen Wang W, Garg S, Prather P, and Hauer-Jensen M "Palmitoylethanolamide regulates development of intestinal radiation injury in a mast cell dependent manner". Digestive Diseases and Sciences, 59(110: 2693-703, 2014. Wang J, Kulkarni A, Chintala M, Fink LM and Hauer-Jensen M, "Inhibition of protease-activated receptor 1 ameliorates intestinal radiation mucositis in a preclinical rat model". International Journal of Radiation Oncology, Biology, Physics, 85(1): 208-15, 2013. Wang J, Boerma M, Kulkarni A, Hollenberg-Morley D and Hauer-Jensen M, "Activation of protease activated receptor 2 by exogenous agonist exacerbates early radiation injury in rat intestine". International Journal of Radiation Oncology, Biology, Physics, 77(4):1206-12, 2010. Boerma M, Wang J, Kulkarni A, Roberto KA, Qiu X, Kennedy RH and Hauer-Jensen M, "Influence of Endothelin 1 receptor inhibition on functional, structural and molecular changes in the rat heart after irradiation". Radiation Research, 170 (30), 275-283, 2008. Wang J, Boerma M, Fu Q., Kulkarni A, Fink LM and Hauer-Jensen M, "Simvastatin ameliorates radiation enteropathy development after localized, fractionated irradiation by a protein C-independent mechanism". International Journal of Radiation Oncology, Biology, Physics, 68(5):1483-90, 2007. Wang J, Zheng H, Kulkarni A, Ou X and Hauer-Jensen M, "Regulation of early and delayed radiation responses in rat small intestine by capsaicin-sensitive nerves". International Journal of Radiation Oncology, Biology, Physics, 64(5): 1528-1536, 2006. Wang J, Qiu X, Kulkarni A and Hauer-Jensen M, "Calcitonin gene-related peptide and substance P regulate the intestinal radiation response". Clinical Cancer Research, 12(13):4112-8, 2006. Wang J, Zheng H, Qiu X, Kulkarni A, Fink LM and Hauer-Jensen M, "Modulation of the intestinal response to ionizing radiation by anticoagulant and non-anticoagulant heparins". Thrombosis and Haemostasis, 94:1054-1059, 2005. Co-authored on more than 9 abstract presentations at professional conferences (list available upon request).
acid, Photoshop, aseptic techniques, biology, cell culture techniques, cell culture, Data Management and Analysis, data analysis, decision-making, DNA, documentation, drug development, ELISA, experiments, focus, GLP, hematology, image processing, Image, imaging, immunology, interpretation, laser, MACS, Microsoft Office, molecular biology, oncology, PCR, Prism, Project Management, quality, real time, research, safety, scientific, validation, Western blotting
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