research assistant i resume example with 3+ years of experience

Jessica Claire
Montgomery Street, San Francisco, CA 94105 609 Johnson Ave., 49204, Tulsa, OK
Home: (555) 432-1000 - Cell: - - - -
Career Overview
Multi-tasking, highly organized and self-motivated Research Assistant and laboratory manager with over four years of experience specializing in Microbiology and Molecular Biology. Comprehensive background in conducting various procedures, including: cloning, DNA, RNA and protein purification, PCR, quantitative real time PCR, tissue culture, transfection, mouse handling and genotyping. Incredibly persistent problem solver with exceptional relationship building and interpersonal communication skills. Superb ability to interpret research outcomes and prepare reports for publications. Strong working knowledge of: MS Office Suite; EndNote; JMP; DNA, RNA and protein sequence analysis tools (NCBI BLAST, SnapGene, Pfam, UniProt) and multiple sequence alignment packages (EMBOSS, ClustalW2 and Jalview).
  • Guest services
  • Inventory control procedures
  • Merchandising expertise
  • Loss prevention
  • Cash register operations
  • Product promotions
Technical Skills
  • M.
  • 2008) Conserved C-Terminal Motifs Required.
  • for Avirulence and Suppression of Cell Death by Phytophthora sojae effector Avr1b.
  • Plant Cell 20:1118-1133.
  • Claire, P.
  • and Gross, D.
  • C.
  • 2010) Characterization of Extracytoplasmic Function Sigma Factors in Plant.
  • Pathogenesis by Pseudomonas syringae pv.
  • syringae B728a.
  • American Phytopathological Society Annual Meeting.
  • C ERTIFICATIONS SAP Supplier, Relationship Management, Purchasing and Receiving (2011) BSL1 and BSL2 Laboratory Safety and Biohazard Compliance (2011) Animal Care and Use in Research (2011) Blood Borne Pathogens Safety (2014) Fire Prevention and Life Safety (2014).
Work Experience
07/2011 to
Research Assistant I Dartmouth College Hanover, NH,
  • Manage day-to-day operational flow of laboratory, including monitoring budgets; overseeing reagents/ chemicals inventories and ordering supplies/ equipment as necessary.
  • Ensure strict quality control and biosafety compliance; develop laboratory policies and standard operating procedures (SOPs) for laboratory and departmental equipment.
  • Supervise and train new laboratory personnel on experimental protocols and equipment usage.
  • Serve as the departmental cell culture facility manager and fire warden.
  • Oversee transgenic mouse colonies in vivarium and genotype mice for experiments.
  • Create and maintain databases of transgenic mice, cryopreserved mammalian cells and bacterial stocks.
  • Maintain primary and immortalized mammalian cell lines in culture under strict aseptic conditions.
  • Extract genomic DNA from transgenic mouse tails for genotyping transgenic mouse strains.
  • Purify RNA from mouse skeletal muscles for subsequent quantitative real time PCR assays.
  • Perform cloning, subcloning and plasmid DNA purifications for transfection and electroporation assays.
  • Isolate and enrich for primary macrophages from mouse spleens and human T cells from whole blood.
  • Accurately record experimental data in daily laboratory notebook to document research findings.
04/2008 to 04/2011
Graduate Research Assistant University Of Minnesota Minneapolis, MN,
  • Generated gene knock out mutants of two sigma factor genes in the plant pathogenic bacterium, Pseudomonas syringae pv.
  • syringae B728a (Pss B728a) by using a site-directed mutagenesis strategy.
  • Determined gene expression levels in wild type and mutant bacteria using quantitative real time PCR.
  • Confirmed mutations in transgenic bacteria via Southern blot, colony PCR and sequence analyses.
  • Identified gene members of the sigma factor regulons by using quantitative real time PCR.
  • Characterized the functional roles of sigma factor genes in Pss B728a by using multiple phenotypic assays.
  • Presented research poster and talk at the American Phytopathological Society meeting and departmental graduate symposia to communicate research findings.
  • Trained new undergraduate and graduate students in laboratory procedures and equipment usage.
  • Maintained bacterial strains in liquid, solid and semi-solid cultures as well as in glycerol stocks.
  • Experienced in anaerobic bacterial culture.
  • Prepared various nutrient-rich and nutrient-deficient growth media for bacterial strains, buffers and solutions.
  • Carried out general laboratory and equipment maintenance; ordered supplies.
08/2007 to 05/2008
Graduate Research Assistant University Of Minnesota Minneapolis, MN,
  • Physiology & Weed Science Determined the roles of bacterial effector genes in suppressing programmed cell death in Arabidopsis thaliana through plant inoculation studies and serial dilution plating of infected leaves.
  • Cloned bacterial avirulence genes in order to delineate their roles in disease formation on plants.
  • Measured pathogenicity of strains on different plant species using bacterial population counts.
  • Designed co-infection assays on plants to study DNA transfer between strains of Pseudomonas.
  • Inoculated plants with bacterial suspensions with syringe, dip and spray techniques.
  • Facilitated bacterial triparental matings to study possible recombinations.
  • Managed bacterial stock cultures and plants in growth chambers.
Work History

Department of Plant Pathology & Microbiology ,

Department of Plant Pathology ,
Education and Training
Expected in 2011 to to
Master of Science: Plant Pathology & Microbiology
TEXAS A&M UNIVERSITY - College Station, TX
GPA: GPA: 89/4.0
Plant Pathology & Microbiology GPA: 89/4.0 Thesis: "Characterization of Two Extracytoplasmic Sigma Factors in Plant Pathogenesis by Pseudomonas syringae pv syringae B728a."
Expected in 2003 to to
Diploma: Computer Applications
Computer Applications
Expected in 1999 to to
Bachelor of Science: Botany (Honors), Zoology & Physiology
Botany (Honors), Zoology & Physiology
Aseptic Technique Southern and Northern Blot Mouse Genotyping PCR Quantitative RT-PCR Protein Purification Site Directed Mutagenesis Transformation T Cell Isolation & Enrichment Gel Electrophoresis Tissue Culture Fluorescence Microscopy Cloning Transfection Laboratory Management DNA and RNA Extraction Mouse Colony Management Biosafety Compliance
Pal, R., Palmieri, M., Loehr, J. A., Li, S., Abo-Zahrah, R., Monroe, T. O., Claire, P., Sardiello, M. and Rodney, G. G. (2014) Nox2 impairs autophagy and lysosome biogenesis through Src kinase in Duchenne muscular dystrophy. Nature Communications 5:4425. Pal, R., Claire, P., Li, S., Minard, C. G. and Rodney, G. G. (2013) Real-time Imaging of NADPH Oxidase Activity in Living Cells using a Novel Fluorescent Protein Reporter. PLoS ONE 8(5): e63989. Claire, P., Vaughn-Diaz, V. L., Greenwald, J. W. and Gross, D. C. (2013) Characterization of Five ECF Sigma Factors in the Genome of Pseudomonas syringae pv. syringae B728a. PLoS ONE 8(3): e58846. Dou, D., Kale, S. D., Wang, X., Chen, Y., Wang, Q., Wang, X., Jiang, R. H. Y., Arredondo, F. D., Anderson, R. G., Claire, P., McDowell, J. M., Wang, Y. and Tyler, B.
Aseptic Technique, budgets, cell culture, databases, DNA, equipment maintenance, experiments, functional, laboratory procedures, Pathogenesis, PCR, personnel, Physiology, policies, protocols, quality control, real time, research, RT-PCR, Sigma, strategy, type

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