Highly skilled Scientist with extensive experience in new product workflow and assay development along with performing fundamental research and providing technical support within the biotechnology industry. 10 years of experience in genomics with expertise in next generation sequencing (NGS) field. Core competencies include:
Effective Communicator
Strong Analytical Skills
Team Player
Technical Depth and Breadth
Strong Interpersonal Skills
Detail Oriented
Technical Writing
Excellent Presentation Skills
Leadership Skills
Skills
Academic
Approach
C
Cost Reduction
Customer support
Data analysis
Designing
Product development
ELISA
Functional
Good Laboratory Practice
GLP
Ink
Innovation
Inventory
Novel
Optimization
PCR
Personnel
Presenting
Presentations
Printers
Process Development
Progress
Protocols
Read
Research
S5
User manuals
Troubleshoot
View
Workflow
Lab Operations
Research design
Conducting Assays
Technical Writing
Experimental design
Work History
Research Assistant, 03/2018 to 05/2018 Rogers Memorial Hospital – Brown Deer, WI,
Perform experiments using mouse models with high HDL levels, tissue harvesting, flowcytometry,
genotyping, PCR, RNA-seq, ChIP-seq, 4C-seq and HiC assays, data analysis and visualization, literature
searches, writing SOPs and protocols for reference by new users, maintaining lab operation and inventory.
Scientist II, 01/2015 to 01/2016 Eurofins Scient. – Rahway, NJ,
Core member of the Clinical Sequencing Division, designing and executing wet-lab experiments and
presenting key findings to the core product development groups to meet product launch deadlines and
advancing workflow procedures.
Supported IVD product development efforts for the Ion Chef and Ion S5 platforms for Clinical use by
early 2017.
Implemented the DOE approach to troubleshoot and optimize the sample prep process, which
resulted in a no cost solution and an on-time release of the Hi-Q view PGM kits in June 2016.
Accurately recorded, analyzed and summarized experimental data, wrote progress reports and
proposed optimization strategies.
Contributed to writing verification reports, user manuals and training documents, while actively
collaborating with cross-functional units.
Scientist I, 01/2009 to 01/2013 Wuxi Apptec – Middletown, DE,
Integral member of the Technology Innovation group, a multi-site team of accomplished scientists, focused
on identifying emerging disruptive technologies for DNA sequencing by collaborating with academic labs
and start-ups.
Also worked with the Assay Development, Cost Reduction and Process Development groups
in the past, improving sequencing performance while adhering to Good Laboratory Practice (GLP).
Worked on early stage technologies such as the Polonator adapting the Illumina sequencing
chemistry for integrating into the development pipeline.
Researched and implemented improvements to the sequencing chemistry on the GS FLX+ platform,
which led to longer read lengths (up to 1000 bp) and higher signal per base on the launched
instrument.
Authored technical documents for internal users and colleagues about improvements to the assays
and presented research findings to cross-functional teams, R&D groups and external collaborators.
Supervised research associates by setting goals, expectations and day-to-day activities, developed
test plans, and guided in data analysis and presentations.
Trained interns, research associates, technical and customer support personnel on all aspects of
library preparation, emulsion PCR and sequencing workflow using the 454 systems.
Sr. Research Specialist, 01/2005 to 01/2008 Gartner – Boston, MA,
Independently performed in vitro experiments, including qPCR, ELISA, Western Blots, Cytokine
panel screens using Luminex and in vivo procedures using rats and mice, to identify novel molecular
biomarkers for the treatment of pulmonary hypertension (PH).
Statistically analyzed and interpreted
the data and presented the results to both internal and external stakeholders.
Education
Master of Science: Bioengineering, Expected in 2004 CLEMSON UNIVERSITY - Clemson, SC GPA: Engineering Three-Dimensional Functional Freeform Structures Using Ink-Jet Printers.
Bachelor of Engineering: Biomedical Engineering, Expected in 2002 MUMBAI UNIVERSITY - Mumbai, GPA:
Accomplishments
Facilitated the development, verification and validation of the Ion Torrent S5 sequencing system, the
simplest, fastest and most affordable sequencer in the market, in the 3rd
quarter of 2015.
Developed a mathematical equation based on empirical findings to calculate the pH of consumables,
saving ~2 hours per day for each lab member, contributing to the company’s key goal of PPI
implementation.
Invented a method to compact isothermally amplified single stranded DNA into distinct "nanoballs" for
sequencing that resulted in a patent publication in Aug 2013.
US20130217023 A1.
Performed fundamental research to improve sample prep workflow by significantly reducing the on-chip
amplification time from 10 hours down to ~2 hours.
This work resulted in a patent issued in Apr 2017.
US 9,624,519 B2.
Conducted a DOE (Design of Experiments) for increasing HIV amplicon generation in low viral load
(
Developed RNA fragmentation and cDNA library construction protocols for the cDNA Rapid Library
Preparation Method Manual released for the GS FLX Titanium platform in 2009.
Set-up an experimental model to assess unique biomarkers and genetic modifiers involved in acute lung
injury at Johns Hopkins University.
This work led to co-authorship on 4 papers published in peer-
reviewed journals and 5 conference abstracts.
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