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Research Assistant Resume Example

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RESEARCH ASSISTANT
Education
2015
Bachelor of Arts: Biochemistry
 Westminster College - Fulton, Missouri

Career GPA 3.76 / 4.00

Major GPA 3.91 / 4.00
Skills

Laboratory Skills

  • PCR
  • ELISA
  • Bio-layer Interferometry
  • Fluorescent Microscopy
  • Cell Transformation
  • Tissue Culture (transfection, gene expression analysis, in vivo assays)
  • RT-qPCR
  • Animal colony and database management
  • Protein Purification  
  • DNA Sequencing
  • Primer Design
  • Mass Spectrometry (LC-MS, TOF)
  • Antibody Staining
  • Fluorescent / Confocal Microscopy
  • Immunohistochemical Staining
  • Flow Cytometry
  • Plasmid Construction and Cloning    
  • Mutagenesis (Site- directed / EP-PCR)
  • Gels (DNA, PAGE, Native)
  • Cell Culture
  • X-ray Crystallography
  • Small Animal Biomethods (injection, tissue collection, surgeries)
  • Animal Colony Maintenance (breeding, welfare, genotyping)           

Technical Skills

  • GROMACS
  • Microsoft Office
  • GraphPad Prism
  • FlowJo
  • ImageJ
  • MATLAB
  • Adobe Creative Suite (Photoshop, Illustrator)
  • Scripting (Python, Bash, MATLAB)    
  • PyMOL
  • Imaris 
Work History
09/2016 to Current
Research Assistant The Jackson Laboratory - Dr. Nadia Rosenthal's Lab – Bar Harbor, Maine

  • Working on several projects aimed at understanding models of regeneration in mice.
  • Preparing and performing flow cytometric analysis in up to 20 colors on various tissues and cell lines.
  • Maintaining cell lines for usage in transfection assays, in vivo assays, RT-qPCR.
  • Constructing plasmid vectors via restriction digest, fragment PCR, ligation, Gibson Assembly, and bacterial transformation for transfections and micro-injections.
  • Collecting, analyzing, and presenting data from experiments for publication.
  • Maintaining mouse colonies: breeding, genotyping, surgeries, and charting pedigrees.
  • Logging frequent and comprehensive notes regarding procedures.
  • Keeping stock of reagents and supplies, sourcing preferred vendors, and frequently placing orders.
  • Completing experiments in a timely manner with reproducible results.
05/2015 to 06/2016
Research Technician II Washington University - Dr. Scott Holtgren's Lab – St. Louis, Missouri
  • Worked on a project characterizing tip adhesins involved in Uropathogenic E. coli (UPEC) infection.
  • Performed a variety of assays to determine binding properties of adhesins.
  • Prepared samples for microscopy.
  • Developed new methods for analysis.
  • Performed Molecular Dynamics simulations to inform further in vitro work.
  • Prepared a variety of buffers, solutions, plates, etc. for the lab, in addition to assisting others as time allowed.
05/2014 to 08/2014
REU Intern in Analytical Research & Development Sigma-Aldrich Corporation – St. Louis, Missouri
  • Optimized protocol for post-labeling cleanup of labeled glycans.
  • Gained skills in SPE chromatography, mass spectrometry, and HPLC.
  • Performed extensive searches through related literature
  • Developed novel protocols.
  • Successfully incorporated several procedures into the accepted glycan labeling workflow.
  • Presented results at student intern poster session. 
08/2014 to 05/2016
Independent Research Project Virology and Cell Culture Project - Westminster College – Fulton, Missouri
  • Supplemental project aimed at acquiring new skills in cell culture and viral biology.
  • Infected BHK cells—an immortal cell line—with Vesicular Stomatitis Virus to produce and purify viral culture in high titers.
  • Presented results at Westminster College Undergraduate Scholars Forum.
Affiliations

Delta Tau Delta Fraternity 

  • Active Member from 2011-2015
  • Philanthropy Chair from 2013-2014
  • House Manager from 2014-2015
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Resume Overview

Companies Worked For:

  • The Jackson Laboratory - Dr. Nadia Rosenthal's Lab
  • Washington University - Dr. Scott Holtgren's Lab
  • Sigma-Aldrich Corporation
  • Virology and Cell Culture Project - Westminster College

School Attended

  • Westminster College

Job Titles Held:

  • Research Assistant
  • Research Technician II
  • REU Intern in Analytical Research & Development
  • Independent Research Project

Degrees

  • Bachelor of Arts : Biochemistry

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