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University of North Carolina - Research Analyst Resume Example

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RESEARCH ANALYST
Career Overview

Researcher with over 14 years of experience in a variety of laboratory settings.

Experienced in good aseptic technique in GLP/GMP environment, variety of cell isolation methods, cell culture, and maintenance of cell lines. Passaging, freezing, thawing of adherent and suspension cells and cell banking. Established cell culture facilities from the ground up at two different sites.

Conducted immunoassays such as immuno-cytochemistry, immuno-histochemistry, ELISA, antibody characterizations, Western Blot analysis, and flow cytometry.

Experienced in molecular biology methods such as plasmid preparations, DNA and RNA isolation, RT-PCR.

Excellent in maintaining laboratory materials and equipment. Experienced in training students and other co-workers in the lab.

Self learner and takes initiative to design experiments, works well independently or in a team environment. Highly motivated, energetic, detail oriented individual and is enthusiastic in learning.

Data preparation and analysis, excellent in record keeping, utilization of MS Office suite.

Qualifications
  • Excellent problem solving skills

  • Excellent in cell culturing, cell based assays and cell isolations from various tissues
  • Very good at following SOP's and conducting various laboratory techniques.

  • Multi-tasking, hard working, lab management.
Technical Skills
Skills Experience Total Years Last Used
Work Experience
University of North Carolina November 2012 to December 2013 Research Analyst
Chapel Hill, NC
  • Conducted experiments in characterization of proteins involved in Insulin signaling pathway.
  • Introduced Western Blot technique to the lab and method of analyzing the results using chemi-luminescence with blot scanner.
  • Set up of new tissue culture facility and introduced cell culture techniques to the lab and trained other lab personnel.
  • Responsible for maintenance of the facility.
  • RNA, DNA analysis on Agilent Bio-analyzer and training of lab personnel with the technique.
  • Involved in the development of next generation sequencing using PRO-seq protocol.
University of North Carolina April 2009 to August 2012 Research Specialist
Chapel Hill, NC
  • Discovered stem cell like cells in human tissue culture experiments that are of non-embryonic origin.
  • Developed an array of methods to isolate and characterize the cells.
  • The studies resulted in applying for a patent on the process.
  • Developed a variety of SOPs for the isolation and characterization of these cells.
  • Techniques utilized for the project: Optimization of cell culture media and culture conditions for the growth of the cells, developed Percoll density gradients to isolate and measure the size of the cells, magnetic cell separation, cell isolation with Invitrogen's Dynabead kit, immuno-cytochemistry, flow cytometry, characterizations using bio-analyzer, FV1000 laser confocal, Zeiss 510 confocal, SP2 confocal, light and fluorescent microscopes.
University of North Carolina August 2007 to March 2009 Research Technician
Chapel Hill, NC
  • Introduced to the lab and developed cell culture techniques with a goal of isolating neural stem cells from Olfactory Epithelial biopsy specimens from patients.
  • Optimized cell culture conditions with various media formulations.
  • Developed inventory of cell bank method.
  • Maintenance of laboratory equipment, ordering of laboratory materials.
GlaxoSmithKline Pharmaceuticals March 2004 to December 2004 Laboratory Technician
Research Triangle Park, NC
  • Rabbit polyclonal antibodies screening by cell based time-resolved fluorescence (cell-TRF) immunoassay and organization of the antibody inventory system.
  • Learned and performed hybridoma technologies including PEG-induced somatic fusion, hybridoma culture, limiting dilution cloning, antibody isotyping and characterization.
  • Performed screening of antibody specificity, and sensitivity by ELISA, cell TRF assay and FMAT competition assay using an automated liquid handling system (Biomek-FX station with Sami software).
  • Extensive cell culture and maintenance of cell inventory.
  • Responsible for the general maintenance of the laboratory and ordering the lab supplies.
Duke Univ. Medical Center August 2001 to July 2003 Research Analyst
Durham, NC.
  • Assisted primary investigators in clinical trial efforts towards vaccines for prostate cancer and renal cell dendritic cell based carcinoma.
  • Performed QA/QC of dendritic cell vaccine products ready for administration to patients.
  • Auditing of SOP documents and batch records.
Memorial Sloan-Kettering Cancer Center October 1999 to June 2001 Research Technician
New York, NY.
  • Assisted in the development of DNA vaccines against Prostate Cancer during the pre-clinical trials in mice.
  • Mini and Maxi DNA preparation and gel electrophoresis of DNA, generating DNA bullets for gene gun.
  • Paraffin tissue preparation and sectioning, extensive experience in immuno-histochemistry.
  • Maintained carcinoma cell lines and did routine cell culture and was responsible for laboratory maintenance.
UT Health Science Center July 1994 to October 1995 Research Assistant
San Antonio, TX.
  • Assisted in developing different Neuro-chemical assays, Shimizu assay (cAMP estimation), phosphoinositol hydrolysis assay, receptor-ligand binding assays, intact cell assay using RIA (techniques utilizing radio-active materials).
  • Tissue culture, column chromatography, responsible for managing the laboratory.
UT Health Science Center July 1992 to March 1994 Research Assistant
San Antonio, TX.
  • Assisted in biological assays of rabbit platelet and human neutrophil function involving sample handling, liquid scintillation counting, data analysis, and routine laboratory procedures.
Education and Training
Bombay University M.S: Zoology Mumbai, India Zoology
Osmania University B.S: Biology Hyderabad, India Biology
Work History
Dept.
Department of Pharmacology
Department of Pathology
Publications
Citizen of the United States Publications: 1. C. Lisa Kurtz, Bailey C. Peck, Emily E. Fannin, Carines Beysen, Sturart R. Landstreet, Shengli Ding, Katrin Plotze, Thomas Waldow, Vandana Turaga, Sudha Biddinger, Scott Turner, P. Kay Lund, Peter E.H. Schwarz, Kasey C. Vickers and Praveen Sethupathy. A Regulatory Role for microRNA-29a in hepatic pathways relevant to type-2 diabetes. Diabetes, 2013 (submitted). 2. Polly Gregor, Jedd D Wolchok, Turaga V. Induction of Autoantibodies to syngeneic Prostate-specific membrane antigen by Xenogeneic Vaccination. International journal of Cancer, 2005 Sep 1;116(3):415-21. 3. Su Z, Vieweg J, Weizer AZ, Dahm P, Yancey D, Turaga V, Higgins J, Boczkowski D, Gilboa E, Dannull J. Enhanced induction of telomerase-specific CD4(+) T cells using dendritic cells transfected with RNA encoding a chimeric gene product. Cancer Res. 2002 Sep 1:62(17):5041-8.
Skills
Auditing, data analysis, ELISA, inventory, laser, managing, materials, neural, next, Optimization, pathway, personnel, QA, radio, scanner, SOP
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Resume Overview

Companies Worked For:

  • University of North Carolina
  • GlaxoSmithKline Pharmaceuticals
  • Duke Univ. Medical Center
  • Memorial Sloan-Kettering Cancer Center
  • UT Health Science Center
  • Dept.
  • Department of Pharmacology
  • Department of Pathology

School Attended

  • Bombay University
  • Osmania University

Job Titles Held:

  • Research Analyst
  • Research Specialist
  • Research Technician
  • Laboratory Technician
  • Research Assistant

Degrees

  • M.S : Zoology
    B.S : Biology

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