Molecular geneticist with broad expertise from molecular biology, biochemistry, plant physiology, system biology to quantitative genetics.
Proficient in using R programming language for QTL mapping, RNA seq and DNA seq data analysis and statistical learning.
RNAseq data analysis
Candidate gene prioritization
Published 17 peer-reviewed papers, including 11 first-authored papers.
Presented research work in 12 scientific conferences.
Reviewed research papers for multiple major journals of the field.
Received a few exceptional awards including: postdoctoral fellowship, the best poster presentation, and honorable mention prize for oral presentation.
Wrote and co-wrote many papers and three grant proposals.
Presented at 13 national/international conferences.
Lectured and supervised undergraduate lab sessions for 9 semesters.
Supervised the research work of 9 undergraduate students.
University of British Columbia Vancovuer, BC, CanadaMolecular biology and genetics in plant Ph.D.
University of Winnipeg Winnipeg, Manitoba, CanadaPlant microbe interaction Master of Science
College of Life Science, South China Normal University Guangzhou, Guangdong, P.R. ChinaPlant physiology Master of Science
Northwest University Xi'an, Shanxi, P.R. ChinaBotany (major degree) Computer science(minor degree) Bachelor of Science
Research skills: ability to identify key problems in the field and find best solutions with resources available.
Biochemistry skills: protein expression and purification, co-immunopercipitation, protein kinase assay, protein ubiquitination assay, protein complex separation using sucrose gradient centrifugation, HPLC, MS.
Molecular biology skills: exome capture, DNA/RNA library preparation for Illuminal sequencing, protoplast transient expression, gene cloning, DNA microarray.
Data analysis skills: R programming, QTL mapping, DNA/RNA sequencing data analysis, statistical learning.
Writing skills: wrote papers for publication in scientific journals and proposal for grant application.
Communication skills: obtained outstanding prizes for oral and poster presentation, lectured and supervised 9 undergraduate lab sessions.
NSERC POSTDOCTORAL FELLOWSHIP NSERC, CANADA, July 2010 - July 2012 (national level competition)
“President's Award” for the Best Poster Presentation Annual Meeting of Canadian Society of Plant physiologists (CSPP), Burnaby, Canada, 2009 (national level competition)
Registration Award 19th International Conference on Arabidopsis Research, Montreal, Canada, 2008 (international level competition)
Oral Presentations Award Honorable Mention prize, Loon Lake Cell Biology Retreat, Vancouver, Canada, 2008 (provincial level competition)
Poster Presentations Award Honorable Mention Prize, Loon Lake Cell Biology Retreat, Vancouver, Canada, 2007 (provincial level competition)
PhD Tuition Fee Award Faculty of Graduate Studies, University of British Columbia, 2005-2009 (university level competition)
Academic Achievement Award South China Normal University, 2002 (University level competition)
National Entrance Examination Waiver for Graduate Schools Ministry of Education, P.R. China, 1999 (national level competition)
Excellent Undergraduate Student Chinese Northwest University, (university level competition) 1996-1999
July 2012 to Current
Carnegie Institution for Science at Stanford, Department of plant biologyStanford, CAPostdoctoral Research Associate
Research topics: Molecular mechanism of plant adaptation to salt stress and nitrogen deficiency conditions.
Major contributions:(1) Designed and developed a novel QTL mapping strategy with maximum QTL detection power and applied the approach in mapping novel QTL of salt tolerance in arabidopsis. (2) Discovered a critical molecular complex mediating nitrate-regulated lateral root development comprised of a deubiquitination enzyme, an activator and a target.
Supervisor: Dr. Sue Rhee
July 2011 to July 2012
Oak Ridge National Laboratory, Bioscience DepartmentOak Ridge, TNNSERC PDF Research Fellow
Research topic: Mechanism of cell wall recalcitrance to enzyme digestion during biofuel production in populus.
Major contributions: (1) Designed and led the development of populus protoplast transient expression system. (2) Applied the system to probe the molecular function of candidate genes ranked by QTL and GWAS mapping approaches in populus.
Supervisor: Dr. Gerald A. Tuskan
July 2010 to July 2011
Department of Genetics, Harvard Medical School, Department of Molecular Biology, MGHBoston, MANSERC PDF Research Fellow
Research topic: Dissecting signaling components of KIN10-mediated stress and energy signaling pathway
Major contributions: (1) Identified three GATA and two bHLH transcription factors which could block the the activation of stress responsive genes by KIN10. (2) Identified a deacetylase, SIRT2, as a core component of KIN10-activated stress-responsive genes.
Supervisor: Dr. Jen Sheen
September 2005 to July 2010
Department of Botany, UBCVancouver, BCPh.D. student
Research topic: Molecular mechanism of plant response to abiotic stress
Major contributions:(1) Characterized the genetic interaction between RACK1 and G-protein in mediating the signaling of the stress hormone, abscisic acid. (2) Identified RACK1 protein family as key component linking the perception of abiotic stress by plant and regulation of protein translation as a respone.
Supervisor: Dr. Jin-Gui Chen
September 2002 to July 2005
Department of Plant Science, University of ManitobaWinnipeg, MannitobaMs.c. student
Research topic: Effect of nitrogen nutrient on legume/rhizobium symbiosis
Major contributions:(1) Identified different effects of nitrate and ammonium on the success of nodule formation in legume. (2) Identified potential plant functional pathways responsible for this difference using DNA microarray approach.
Supervisor: Dr. J. Kevin Vessey
September 1999 to July 2002
College of Life Science, South China Normal University, Guangzhou, P.R. ChinaGuangzhou, GuangdongMs.c. student
Research topic: Effect of hormone and cold treatment on flowering time of orchid
Major contributions:(1) Identified an optimal cold treatment period for effective floral onset promotion. (2) Pinpointed Gibberellin as the major plant hormone involved in this process.
Supervisor: Dr. Qingshen Ye
Visa status: TN (Trade NAFTA) status
ASPB member since 2009
AAAS member since 2006
Guo J*, Fan J*, Hauser B, Rhee S (2014) Mapping complex traits by deep sequencing target-enriched markers of bulked F2 segregants in Arabidopsis thaliana. Genetics (In revision) *equally contributing
Muchero W, Guo J, et al. (2014) Multi-environment identification of allelic variants associated with cell wall chemistry in populus. BMC Genomics (In revision)
Guo J, Morrell-Falvey JL, Labbé JL, Muchero W, Kalluri UC, Tuskan G, Chen J (2012) Highly efficient isolation of populus mesophyll protoplasts and its application in transient expression assays. PLoS ONE 7: e44908.
Wang S, Chang Y, Guo J, Zeng Q, Ellis BE, Chen J (2011) Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development. PLoS ONE 6(8): e23896
Guo J, Jin Z, Yang X, Li J-F, Chen JG (2011) Eukaryotic initiation factor 6, an evolutionarily conserved regulator of ribosome biogenesis and protein translation. Plant Signal Behav 6: 766-771
Guo J,Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG (2011) Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid. Plant Physiol 155: 370-383
Guo, J, Wang S, Wang J, Huang WD, Liang J, and Chen JG (2009) Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis. Plant Cell Physiol 50:1681-1694
Guo J,Wang J, Xi L, Huang WD, Liang J, and Chen JG (2009) RACK1 is a negative regulator of ABA responses in Arabidopsis. J Exp Bot 57: 2697-2708
Guo J, Chen JG (2008) RACK1 genes regulate plant development with unequal genetic redundancy in Arabidopsis. BMC Plant Biology 8: 108
Guo J, Zeng Q, Emami M, Ellis BE, Chen JG (2008) The GCR2 gene family is not required for ABA control of seed germination and early seedling development in Arabidopsis. PLoS ONE 3: e2982.
Wang S, Hubbard L, Chang Y, Guo J, Schiefelbein J, Chen JG (2008) Comprehensive analysis of single-repeat R3 MYB proteins in epidermal cell patterning and their transcriptional regulation in Arabidopsis. BMC Plant Biol 8: 81.
Wang S, Chang Y, Guo J, Chen JG (2007) Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation. Plant J 50: 858-872.
Gao Y, Zeng Q, Guo J, Cheng J, Ellis BE, Chen JG (2007) Genetic characterization reveals no role for the reported ABA receptor, GCR2, in ABA control of seed germination and early seedling development in Arabidopsis. Plant J 52:1001-1013
Guo J, Liang J, Chen JG (2007) RACK1: a versatile scaffold protein in plants? Intl J Plant Dev Biol 1: 95-105.
Chang Y, Guo J, Gao Y, Chen JG (2007) Modulation of root cell division by the heterotrimeric G-proteins in Arabidopsis. Dyn Cell Biol 1: 72-77.
Chen JG, Ullah H, Temple B, Liang J, Guo J, Alonso JM, Ecker JR, Jones AM (2006) RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis. J Exp Bot 57: 2697-2708.
Huang S, Guo J, Ye Q, Li L (2003) The effect of different diurnal temperatures on the flowering time and floral development of Phalaenopsis orchid. Acta Scientiarum Naturalium Universitatis Sunyatseni 42: 132-136. (In Chinese)
Guo J, Ye Q, Li L (2002) The signalling events and molecular mechanisms of GA's regulation on -amylases' gene expression in cereal grains. Chinese Bulletin of Botany 19: 63-69. (In Chinese)
Li L, Zhang C, Guo J (2001) Practical skill in biology. Hu'Nan Science and Technology Press. (A Chinese translation of: Jones A, Reed, R, Weyers J (1998) PRACTICAL SKILL IN BIOLOGY Addison Wesley Longman Limited) (In Chinese)
Guo J, Fan J, Hauser B, Rhee S (2014) Mapping complex traits by deep sequencing target-enriched markers of bulked F2 segregants in Arabidopsis thaliana. (Oral presentation) Carnegie Retreat, Tahoe, NV, USA
Guo J, Fan J, Hauser B, Rhee S (2014) Mapping complex traits by deep sequencing target-enriched markers of bulked F2 segregants in Arabidopsis thaliana. (Poster presentation) 24th International Conference on Arabidopsis Research, Vancouver, Candada
Guo J, Fan J, Rhee S(2014) Target-enrichment-based extreme QTL mapping for uncovering comprehensive genetic architectures underlying complex traits (Poster presentation). Western ASPB conference, Santa Clara, CA, USA.
Guo J, Muchero W, Morrell-Falvey J, Labe J, Chen JG, Tuskan AG (2012) Post-GWA dissection of transcriptional regulation of secondary cell wall biosynthesis using Populus protoplast transient expression system. 2012 Keystone Symposia, Nuclear Event in plant gene expression and signaling, Taos, NM, USA (Poster Presentation)
Guo J, Wang S, Valerius O, Hall H, Zeng QN, Ellis B, Chen JG (2010) Involvement of arabidopsis RACK1 in protein translation and its regulation by abscisic acid. 2010 Keystone Symposia, Receptors and Signaling in Plant Development and Biotic Interactions, Tahoe, NV, USA (Poster Presentation)
Guo J, Wang J, Huang W, Liang J, Chen JG (2009) RACK1 negatively regulates ABA responses via hindering the desensitization of triggered ABA signalling cascades. 20th International Conference on Arabidopsis Research, Edinburgh, Scotland, June 2009. (Poster Presentation)
Guo J, Wang J, Xi L, Huang WD, Liang J, Chen JG (2009) RACK1 negatively regulates ABA responses during seed germination and early seedling development in Arabidopsis. Canadian Society of Plant Physiologists (CSPP) 2009 Annual Meeting, Burnaby, British Columbia, Canada, June 2009. (Poster Presentation)
Guo J, Chen JG (2008) Arabidopsis RACK1 genes regulate plant development with unequal genetic redundancy. 19th International Conference on Arabidopsis Research, Montreal, Quebec, Canada, June 2008. (Poster Presentation)
Guo J, Chen JG (2008) RACK1 genes regulate stem cell maintenance in Arabidopsis root. 2008 Loon Lake Cell Biology Retreat, Vancouver, British Columbia, Canada, May 2008. (Oral Presentation)
Guo J, Chen JG (2007) A signalling complex constituted by the heterotrimeric G-proteins and the scaffold protein RACK1. 18th International Conference on Arabidopsis Research, Beijing, China, June 2007. (Poster Presentation)
Guo J, Chen JG (2007) Arabidopsis RACK1 proteins act synergistically to regulate rosette leaf production and flowering time. 2007 Loon Lake Cell Biology Retreat. Vancouver, British Columbia, Canada, May 2007. (Poster Presentation)
Guo J, Chen JG (2007) RACK1 gene family act synergistically in controlling Arabidopsis rosette leaf production and flowering time. UBC Botany Graduate Student Symposium, Vancouver, British Columbia, Canada, April 2007. (Oral presentation)
Guo J, Chen JG (2006) RACK1-a scaffold protein in plants? UBC Botany Graduate Student Symposium, Vancouver, British Columbia, Canada. April 2006. (Oral Presentation)
Guo J, Vessey JK (2004) Differential effects of low concentrations of ammonium and nitrate on growth and nodulation in Medicago truncatula. 19th North American Conference on Symbiotic Nitrogen Fixation, Bozeman, Montana, USA. June 2004. (Poster presentation)