ORISE Fellow/Leidos Biological Laboratory TechnicianPlum Island Animal Disease Center DHS － Plum Island, NY
Clone, amplify and purify recombinant vaccine candidates including DNA vaccines and Baculovirus constructs.
Develop sandwich ELISA assay to quantify Virus-Like-Particles (VLPs) produced in various expression systems.
Obtain necessary security and select agent clearance(s).
Perform research using select agents and fulfill the requirements for maintenance of accurate select agent inventories regulated by federal laws.
Complete western blot (immunoblotting) analysis on vaccine samples/constructs in support of DHS S&T contracts.
Conduct immunofluorescent pathogen recovery titration assay and assist in data analysis for the completion of semi-truck decontamination project.
Maintain various/multiple cell culture lines to support viral propagation and titration.
Propagate and titrate viral stocks of veterinary viral pathogens including Foot and Mouth Disease virus.
Test potential viral surrogates, such as Feline Calicivirus and MS2 Bacteriophage, in disinfection cytotoxicity and neutralization assays as well as quantitation of virus recovery experiments.
Provide support to the DHS Transboundary Animal Disease team during in vivo clinical studies with livestock, particularly swine and cattle, by assisting with data collection and record keeping.
Assist in the processing of clinical specimens, including blood, tissue and swabs, from cattle and swine.
Learn and perform sanger sequencing reaction to analyze DNA sequences.
Complete all trainings required to enter BSL-2 and BSL-3/BSL-3 Ag laboratories and work with Select Agent materials.
Demonstrate good record keeping techniques and strong written communication skills by maintaining complete and accurate records in laboratory notebook.
Order supplies and autoclave waste to maintain the laboratories and ensure a clean and safe working environment.
Work independently and/or with a team to troubleshoot experimental flaws and devise alternative methods to solve problems.
Follow DHS rules, regulations, and standard operating procedures (SOPs) in accordance with appropriate laboratory operations.
Review relevant scientific literature to design experiments or improve current experimental procedures.
Attend Introductory to Immunology course provided by the American Association of Immunologists to update and expand understanding in immunology
12/2013 to 01/2015
Research AssociateRockville, MD
Assisted in the development, implementation and performance of various assays and provided support for internal and external vaccine/product translational development of tuberculosis vaccines.
Frequently cloned and transformed bacteria for the purpose of purifying recombinant His-tagged protein using Nickel resin and columns.
Concentrated and quantified recombinant proteins.
Implemented new indirect ELISA assay to quantify protein in vaccine preparations.
Completed ELISA testing on vaccine clinical trial samples as necessary.
Developed and optimized fluorescent DNA quantification assay.
Routinely performed ELISA assays, viral titration using a focus forming unit assay, quantitative PCR (qPCR), Real-Time PCR (RT-PCR), SDS page and Western Blot analysis, BCA assays, and cell culture according to SOPs in BSL-2 laboratory.
Wrote SOPs as new assays were developed.
Followed Good Laboratory Processes (GLP) and Good Manufacturing Processes (GMP) in accordance with Aeras Quality Assurance regulations.
Read and reviewed SOPs to monitor for updates and ensure adherence to protocol.
Regularly attended job related training including Chemical, Biological and Bloodborne Pathogen safety trainings.
Trained in Quality Assurance programs such as ISO 9001.
Performed routine maintenance on laboratory equipment to monitor proper function and prevent damage.
Operated and maintained laboratory equipment, including thermocyclers and ELISA plate readers, according to SOPs.
Prepared reports and presented findings in meetings using strong oral communication skills to discuss results and future experiments.
08/2011 to 08/2013
Graduate Research AssistantUniversity of Connecticut － Storrs, CT
Developed and ethically tested the efficacy of two recombinant vaccines: a plasmid based DNA vaccine and an adenoviral-vectored vaccine against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) developed in a
Evaluated the effectiveness of vaccines based on samples collected during the vaccine/challenge trials in large animals and mouse models using virus titration, antibody neutralization, RNA and DNA isolation/ purification for RT-PCR and qPCR, ELISpot, and BrdU Cell Proliferation Assays.
Grew and propagated hybridoma cell lines for antibody production and analysis.
Established ELISA assays to measure antibody responses in specific animals.
Identified and resolved common problems by improving research techniques and procedures.
Instructed and trained new graduate and undergraduate researchers in the use of equipment and performance of assays.
Collaborated with principal investigators, graduate students and staff in other laboratories to design and conduct experiments.
Maintained laboratory organization, cleanliness, adequate supplies of standard reagents, common lab stock and equipment.
Managed laboratory cell culture, plasmid, freezer, reagent and general supplies inventories and ordered supplies as necessary.
Prepared written communications for research summaries after gathering, analyzing, and interpreting data.
Documented complete and comprehensive records in laboratory notebook and computerized databases for study integrity.
Analyzed data using statistical analysis in GraphPad Prism.
Routinely presented findings in laboratory meetings and group seminars.
Master of Science: Pathobiology and Veterinary Science, VirologyUniversity of Connecticut － Storrs, CTPathobiology and Veterinary Science, Virology
Bachelor of Science: Animal Science & TechnologyUniversity of Rhode Island － Kingston, RIAnimal Science & Technology
BCA, Cell Culture, oral communication, contracts, data analysis, data collection, databases, DNA, ELISA, experiments, focus, GMP, GLP, Immunology, ISO 9001, laboratory equipment, Manufacturing Processes, materials, meetings, Microsoft Office, optimization, Page, Pathogen, PCR, Prism, Processes, Quality Assurance, Read, Real-Time, record keeping, research, RT-PCR, safety, scientific, seminars, statistical analysis, troubleshoot, tuberculosis, vaccine, vaccines, written communications, written communication skills