Dedicated ASCP Medical Laboratory Scientist experienced in Chemistry, Hematology, Coagulation, Urinalysis, Serology, Bacteriology and Immunohematology.
Effectively managed and executed large research collaborations for 8 years with National Institute of Allergies and Infectious Disease (NIAID), Radiation Oncology Branch (ROB), and Radiation Biology Branch (RBB) within the National Institutes of Health (NIH) organization.
Proficient in CLIA, CAP, AABB, OSHA, and FDA requirements for medical laboratory work.
Quality-focused and collaborative team player with reputation for accuracy and efficiency.
Lead operator on PCR Based Molecular System
Develop, implement and evaluate SOP's for new and current assays and procedures and develop IQCP (Individualized Quality Control Plan) Program for PCR based Molecular System assays
Investigate and resolve technical, instrument, and other variances leading to problems with or unexpected test results.
Identify problems which may adversely affect test performance or reporting of test results and takes appropriate action, including medical director notification if indicated.
Conduct experimental studies and perform complex procedures and techniques such as: rodent radiation, cell culture techniques, EPR, DNA extraction and immunohistochemistry and immunofluorescence.
Maryland Primary Care PhysiciansHanover, MarylandMedical Laboratory Scientist08/2017 to Current
Conduct testing to including but not limited to; Hematology automation and manual methods, Chemistry, Serology, Coagulation, Immunoassay (LYME, MMRV, HIV, Hepatitis Profiles, Syphilis, HSV1/2), Molecular GAS and HSV/VZ, Molecular PCR (Vaginal panel, CDIFF, Bacterial Panel, Parasite Panel and CT/GC/TV) and Urinalysis automation and manual methods.
Lead operator on PCR based Molecular System to implement assay validation, LOINC and CPT codes research, LIS assay ordering verification and maintain inventory supply and performs validation of new assays and materials as required for analyzer.
Designee to develop, implement and evaluate SOP's for new and current assays and procedures and develop IQCP (Individualized Quality Control Plan) Program for PCR based Molecular System assays.
Performs and documents all maintenance on equipment required in order to perform the assigned testing accordingly, training all laboratory staff and troubleshooting and assisting staff as needed.
Designee as a lead operator on Centaur XPT Immunoassay system and assists in developing, implementing and evaluating SOP's new and current assays and procedures, training and assisting lab staff with troubleshooting.
Interprets quality control results and instrument function.
Adheres to departmental policies and procedures to include departmental programs, quality control, quality assurance, and safety.
Participates in surveys, conducts established quality control of procedures, evaluates results, implements corrective action when indicated, and ensures accuracy of test results.
Follows laboratory procedures for specimen handling and processing, test analysis, reporting, and maintaining records of patient results.
Identifies problems which may adversely affect test performance or reporting of test results and takes appropriate action, including medical director notification if indicated.
Understands and follows all safety, infection control, and OSHA Bloodborne Pathogen guidelines.
Demonstrates efficiency by performing procedures rapidly and determining priority and utilizes appropriate supplies with minimal waste.
U Of MD Charles Regional Medical CenterLaPlata, MDMedical Laboratory Scientist04/2016 to 07/2017
Conduct high complexity testing including but limited to; Chemistry, Hematology automation and manual methods, Immunhematology automation and manual methods, Toxicology, Serology/Immunology, Coagulation, Urinalysis and body fluids automation and manual methods.
Inoculate clinical specimens from blood, throat, urine, wounds, eyes, feces, etc, for microbiological analysis on appropriate media and perform gram stain analysis.
Perform numerous complicated tests simultaneously and accurately interpreting results within expected turn around time.
Identify normal and abnormal cell morphology on blood and body fluids.
Ensures all testing and processes adhere to CLIA, CAP, OSHA, FDA, and AABB regulations as indicated for each technical area of supervision.
Evaluate quality control within laboratory using standard laboratory test and measurement controls, and maintained compliance with CLIA, OSHA safety and risk-management guidelines.
Perform quality assurance and lab safety functions.
Ensure test-result validity before recording and reporting results.
Leidos Biomedical ResearchBethesda, MDSenior Laboratory Animal Technician11/2007 to 07/2015
Conducted experimental studies and perform complex procedures and techniques such as: rodent radiation, cell culture techniques, EPR, DNA extraction and immunohistochemistry and immunofluorescence on paraffin embedded and frozen section slides.
Colaborated with internal branches such as; ROB with Dr.
Camphausen and Dr.
Citrin, and NIAID.
Delivered radiation doses to mice using Cesium-137, Cobal 60 and Pantek X-ray unit.
Performed basic necropsy, tumor measuring and monitoring and observation and recording of physical signs of animal health.
Conducted technical procedures, such as administration of experimental compounds via various routes, collection of blood and tissue specimens, handling and performing work with hazardous chemicals, biological or radiological materials and assist in surgical procedures.
Managed rodent breeding colonies, genetically complex strains and associated record keeping, and specialized technical procedures including: tail clipping, animal identification, weaning and collection of routine diagnostic samples, record keeping, database management and reporting.
Inspect, collect, interpret, analyze and compile data obtained from experiment to form logical conclusions.
Followed all applicable regulations, policies, procedures and advice principle investigators of standards governing animal welfare.
Education and Training
Bachelor of Science:Medical Laboratory Science2019Austin Peay State University, Clarksville, TN
Associate of Applied Science:Medical Laboratory Technology2016College of Southern Maryland, La Plata, MD
ASCP Certified Medical Laboratory Scientist
Cerfication Number: Provide upon request
Chung EJ, Sowers A, Thetford A, McKay-Corkum G, Chung SI, Mitchell JB, Citrin DE. Mammalian Target
of Rapamycin Inhibition with Rapamycin Mitigates Radiation-Induced Pulmonary Fibrosis in a Murine
Model. Int J Radiat Biol. 2016 Nov.
Cook JA, Chandramouli GV, Anver MR, Sowers Al, Thetford A, Krausz KW, Gonzalez FJ, Mitchell JB,
Patterson AD. Mass Spectrometry-Based Metabolomics Identifies Longitudinal Urinary Metabolite Profiles
Predictive of Radiation-Induced Cancer. Cancer Res. 2016 Mar.
Citrin DE, Shankavaram U, Horton JA, Shield W 3rd, Zhao S, Asano H, White A, Sowers A, Thetford A,
Chung EJ. Role of Type II Pneumocyte Senescence in Radiation-Induced Lung Fibrosis. J Natl Cancer
Inst. 2013 Oct 3
Horton JA, Chung EJ, Hudak KE, Sowers A, Thetford A, White AO, Mitchell JB, Citrin DE. Inhibition of
Radiation-Skin Fibrosis with Imatinib. Int J Radiat Biol. 2013 March.
Mitchell JB, Anver MR, Sowers AL, Rosenberg PS, Figueroa M, Thetford A, Krishna MC, Albert PS, Cook
JA. The antioxidant Tempol reduces carcinogenesis and enhances survival in mice when administered
after nonlethal total body radiation. Cancer Res. 2012 Sep 15.
Flores-Santana W, Moody T, Chen W, Gorczynski MJ, Shoman ME, Velezquez C, Thetford A, Mitchell JB,
Cherukuri MK, King SB, Wink DA. Nitroxide derivatives of non-steroidal anti-inflammatory drugs exert
anti-inflammatory and superoxide dismutase scavenging properties in A459 cells. Br J Pharmacol. 2012
Cotrin AP, Yoshkawa M, Sunshine AN, Zheng C, Sowers AL, Thetford AD, Cook JA, Mitchell JB, Baum BJ.
Pharmacological Protection from Radiation /- Cisplatin-Induced Oral Mucositis. Int J Radiat Oncol Biol
Phys. 2011 Dec 22.
Fabre KM, Saito K, DeGraff W, Sowers AL, Thetford A, Cook JA, Krishna MC, Mitchell JB. The effects of
resveratrol and the selected metabolites on the radiation and antioxidant response. Cancer Biol Ther.
2011 Nov 15.
Urick ME, Chung EJ, Shield WP 3rd, Gerber N, White A, Sowers A, Thetford A, Camphausen K, Mitchell J,
Citrin DE. Enhancement of 5-Fluorouracil-induced In Vitro and In Vivo Radiosensitization with MEK
Inhibition. Clin Cancer Res. 2011 Aug 1.
Davis RM, Sowers AL, Degraff W, Bernardo M, Thetford A, Krishna MC, Mitchell JB. A Novel Nitroxide is
an effective brain redox imaging contrast agent and in vivo radioprotector. Free Radic Biol Med. 2011 Aug
Flores-Santana W, Moody T, Chen W, Gorczynski MJ, Shoman ME, Velazquez C, Thetford A, Mitchell JB,
Cherukuri MK, King SB, Wink DA. Redox-Modified Non-Steroidal Anti-Inflammatory Drugs as Potential
Anti-Cancer Agents with the SOD Mimetic Nitroxide. Br J Pharmacol 2011 Jun 9.
Brown, AP, Chung EJ, Urick ME, Shield WP 3rd, Sowers AL, Thetford A, Shankavaram UT, Mitchell JB,
Citrin DE. Evaluation of the fullerence compound DF-1 as a radiation protector. Radiat Oncol 5: 34, 2010.