Highly motivated biologist with strong molecular skills and expertise in Next Generation Sequencing. Experienced in lab management and project management. Interested in implementing new technologies in daily lab routines. Goal oriented and dedicated to overall lab success and team building.
in handling and preparation of various samples for NGS; target enrichment
Operating HiSeq2500, MiSeq and NextSeq; TruSeq DNA and RNA-Seq library preps, quality control and troubleshooting
PacBio SMRTbell template prep from gDNA, BACs, plasmids and amplicons, IsoSeq prep
Operating PacBio RSII and Sequel
Proficiency in molecular techniques for DNA and RNA isolation, PCR, RT-PCR, cloning
Automation: Caliper Sciclon and Zephyr, Wafergen Apollo, QIASymphony, QIAcube, QIAgility, BluePippin, Pippin Prep, SageELF, TapeStation, Fragment Analyzer, Covaris LE220
Hands-on experience with Oxford Nanopore technology
Laboratory Technician/Laboratory Manager, 12/2010 to Current Company Name – City, State
Serve as a technical lead; support
implementation of new products and procedures in order to improve lab
performance; train lab personnel and interns
compliance of the lab with safety regulations; train new personnel on lab
Coordinate procurement of new equipment and
supplies; guide laboratory infrastructure
Safety Division, Equipment
Management Brunch, Facilities Management Office
Develop standard operating procedures; implement
new methods and workflows
Maintain stock of reagents and consumables;
manage laboratory equipment
Participate in group meetings and
presentations; assist scientists in writing reports and manuscripts
to following projects:
of USAMRIID viral stocks
Comparative genomic and transcriptomic
studies of pathogenic bacterial strains; sequenced hundreds of
bacterial genomes, including novel species
Deep characterization of
Rousettus aegyptiacus (Egyptian fruit bat) immune system
Utility of the microbiome in chemical
Characterization of B-cell repertoire in
humans and non-human pirmates during vaccination
MHC genotyping of non-human primates using
PacBio platform (resulted in award-winning publication)
Exploring single cell technology,
particularly ICELL8 platform from Takara
Direct RNA sequencing on MinION (Oxford
Research Associate II, 02/2008 to 11/2010 Company Name – City, State
Isolated viral fraction from human saliva via ultracentrifugation
Extracted DNA/RNA from various samples and prepared sequencing libraries
projects that studied:
Genomics of multi-drug
resistant isolates of Acinetobacter
Leptospira genomics and
Human oral microbiome
Role of HPV in oral cancer
Biotechnologist, 02/2006 to 02/2008 Company Name – City, State
Performed DNA sequencing on ABI 3730xl platform.
Operated and maintained robotic instrumentation and automated DNA sequencers.
Tracked sample status and results using LIMS; performed quality assessment of data and reported process deviations; provided initial troubleshooting solutions.
Master of Science: MicrobiologyMoscow M.V. Lomonosov State University - City
Bachelor of Science: BiologyMoscow M.V. Lomonosov State University - City, State
1. Stephanie S. Pavlovich, Sean P. Lovett, Galina Koroleva, Jonathan C. Guito,
Catherine E. Arnold, Elyse R. Nagle, Kirsten Kulcsar, Albert Lee, Françoise
Thibaud-Nissen, Adam J. Hume, Elke Mühlberger, Luke S. Uebelhoer, Jonathan S. Towner,
Raul Rabadan, Mariano Sanchez-Lockhart, Thomas B. Kepler, Gustavo
Palacios. The Egyptian rousette genome
reveals unexpected features of bat antiviral immunity. Manuscript has been accepted at Cell.
Chance, Jennifer Chua, Ronald G. Toothman, Jason T. Ladner, Jonathan E. Nuss,
Jo Lynne Raymond, Fabrice V. Biot, Samandra Demons, Lynda Miller, Stephanie
Halasohoris, Sherry Mou,Galina Koroleva, Sean Lovett, Gustavo Palacios, Nicholas
J. Vietri, Patricia L. Worsham, Christopher K. Cote, Todd M. Kijek, Joel A. Bozue. A
spontaneous mutation in kdsD, a biosynthesis gene for 3
Deoxy-D-manno-Octulosonic Acid, occurred in a ciprofloxacin resistant strain of
Francisella tularensis and caused a high level of attenuation in murine models
PLoSONE 12(3): e0174106 https://doi.org/10.1371/journal.pone.0174106
Tuanyok, Mark Mayo, Holger Scholz, Carina M. Hall, Christopher J. Allender,a
Mirjam Kaestli, Jennifer Ginther, Senanu Spring-Pearson, Molly C. Bollig,
Joshua K. Stone, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Jason
W. Sahl, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Jay E. Gee, Richard A. Bowen, Jason T. Ladner,
Sean Lovett, Galina Koroleva, Gustavo Palacios, David M. Wagner, Bart J.
Currie, Paul Keim. Burkholderia
humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis
and the Fifth Member of the Burkholderia pseudomallei Complex . Appl
Environ Microbiol. 2017 Mar 1; 83(5): e02802-16
4. Sean Lovett, Kitty Chase, Galina Koroleva, Gustavo Palacios, David Rozak, and Jason T. Ladner . Complete
Genome Sequence of Pigmentation-Negative Yersinia pestis Strain Cadman
2016 Sep-Oct; 4(5): e01207-16.
W. Sahl, Adam J. Vazquez, Carina M. Hall, Joseph D. Busch, Apichai Tuanyok,
Mark Mayo, James M. Schupp, Madeline Lummis, Talima Pearson, Kenzie Shippy,
Rebecca E. Colman, Christopher J. Allender, Vanessa Theobald, Derek S.
Sarovich, Erin P. Price, Alex Hutcheson, Jonas Korlach, John J. LiPuma, Jason
Ladner, Sean Lovett, Galina Koroleva, Gustavo Palacios, Direk Limmathurotsakul,
Vanaporn Wuthiekanun, Gumphol Wongsuwan, Bart J. Currie, Paul Keim, David M.
Wagne. The Effects
of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic
mBio. 2016 Sep-Oct; 7(5): e00846-16.
Published online 2016 Sep 20. doi: 10.1128/mBio.00846-16
L. Johnson, Kimberly A. Bishop-Lilly, Jason T. Ladner, Hajnalka E. Daligault,
Karen W. Davenport, James Jaissle, Kenneth G. Frey, Galina I. Koroleva, David
C. Bruce, Susan R. Coyne, Stacey M. Broomall, Po-E Li, Hazuki Teshima, Henry S.
Gibbons, Gustavo F. Palacios, C. Nicole Rosenzweig, Cassie L. Redden, Yan Xu,
Timothy D. Minogue, Patrick S. Chain. Complete
Genome Sequences for 59 Burkholderia Isolates, Both Pathogenic and Near
Genome Announc. 2015 Mar-Apr; 3(2):
e00159-15. Published online 2015 Apr 30. doi: 10.1128/genomeA.00159-15
E. Daligault, S. L. Johnson, K. W. Davenport, T. D. Minogue, K. A.
Bishop-Lilly, S. M. Broomall, D. C. Bruce, S. R. Coyne, K. G. Frey, H. S.
Gibbons, J. Jaissle, G. I. Koroleva, J. T. Ladner, C.-C. Lo, C. Munk, M. J.
Wolcott, G. F. Palacios, C. L. Redden, C. N. Rosenzweig, M. B. Scholz, P. S.
Genomes for Eight Burkholderia mallei Isolates from Turkey . Genome Announc. 2016 Jan-Feb; 4(1):
e01234-15. Published online 2016 Jan 7. doi: 10.1128/genomeA.01234-15
8. Westbrook CJ, Karl JA, Wiseman RW, Mate S, Koroleva G, Garcia K, Sanchez-Lockhart M, O'Connor DH, Palacios G.
required: Full-length MHC class I allele discovery by PacBio circular consensus
sequencing. Hum Immunol. 2015
Dec;76(12):891-6. doi: 10.1016/j.humimm.2015.03.022. Epub 2015 May 28.
9. Kugelman JR, Johnston SC, Mulembakani PM, Kisalu N, Lee MS, Koroleva G, McCarthy SE, Gestole MC, Wolfe ND, Fair JN, Schneider BS, Wright LL, Huggins J, Whitehouse CA, Wemakoy EO, Muyembe-Tamfum JJ, Hensley LE, Palacios GF, Rimoin AW. Genomic
variability of monkeypox virus among humans, Democratic Republic of the Congo.
Emerg Infect Dis. 2014 Feb;20(2):232-9. doi: 10.3201/eid2002.130118.
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Moscow M.V. Lomonosov State University
Moscow M.V. Lomonosov State University
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Laboratory Technician/Laboratory Manager
Research Associate II
Master of Science : Microbiology Bachelor of Science : Biology
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