Lab Specialist I Resume Example
- https://www.linkedin.com/in/rolando-mendez-41169822/
Laboratory research professional with 11 years of experience working in translational and clinical research laboratories. Motivated by a childhood curiosity to understand how things work, I have been attracted to academic lab settings focused on cutting edge research to better understand underlying mechanisms of disease and to design and improve treatments for patients. I have used my critical thinking, research and experimental skills to efficiently implement and design new experimental protocols, analyze data, and formulate and suggest subsequent experimental approaches for various projects. My desire to learn and my passion for team work have driven me to take initiative and learn a variety of new experimental techniques, take on multiple projects, and foster collaborative environments that help progress projects and generate new ideas.
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- Adherent cell tissue culturing
- Conducting radioligand binding assays including saturation assays, competition assays, and [35S]GTPgammaS assays
- Performing other molecular assays such as Western blotting and ELISAs
- Coordinating the care of the lab's research mice, treating mice with adverse health events as instructed by the veterinarian, conducting routine colony census, and ordering animal transfers
- Humanely euthanizing experimental mice, extracting whole brains and spinal cords, and dissecting mouse brains for specific regions
- Preparing drugs, buffers, and reagents
- Training and supervising graduate students and technicians in the lab
- Utilizing Excel and Prism for recording and analyzing data
- Coordinating equipment maintenance and ordering and organizing supply inventories
- Ensuring compliance with safety procedures and guidelines related to handling, storage and disposal of bio-hazardous, radioactive, and toxic material
- Acquired and collected cell lines and tissue specimens
- Adherent cell and ex-vivo tumor tissue culturing
- Established cell lines from fresh tumors
- Employed molecular methods such as immunofluorescence assays, immunoprecipitations, siRNA knockdowns, colorimetric assays, flow cytometry, RT-qPCR, and Western blotting
- Performed viral transductions and transfections of mammalian cells
- Irradiated plated cells
- Prepared cells, reagents, and solutions for cell culture experiments and for in vivo experiments
- Trained and supervised new students, residents, and postdocs in the lab
- Assisted with the design of experimental procedures, protocols, and assays to address questions that we have posed or that have arisen during other experiments
- Utilized Excel, Origin, Prism, and other applications for recording and analyzing data, power points, figures, and figure legends, and assisted primary investigator with suggestions and edits of manuscripts
- Coordinated equipment maintenance and ordered and organized supply inventories
- Ensured compliance with safety procedures and guidelines related to handling, storage and disposal of bio-hazardous, infectious and toxic material
- Conducted literature searches using PubMed, Google, Virgo, and UVa's E-Journal
- Worked with various toxic and corrosive gases
- Collected and assayed the pH and redox potential of various biological samples
- Performed spectrometry to measure absorbance
- Assisted with the design of experimental procedures, protocols, and assays to address questions that we have posed or that have arisen during other experiments
- Developed and implemented quality controls, performed or coordinated maintenance of equipment, organized and tracked supply inventories, and trained other student workers how to use lab equipment and carry out experiments
- Conducted literature searches
- Solved mathematical and physics equations involving algebra and calculus to model the relevant problems in the Java program
- Scripted the Java-based program integrating exomoon orbits to characterize their orbital stability and modifying this program to analyze and accommodate for the effects of tidal heating on the moon
- Presented findings at the NASA Virginia Space Grant Consortium Conference at Hampton University (2009)
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Resume Overview
School Attended
- University Of Virginia
Job Titles Held:
- Lab Specialist I
- Lab Assistant
- Research Assistant
Degrees
- Bachelor of Science in Chemistry With Biochemistry Specialization
