Graduate Research student Resume Example

A passionate, qualified and results-driven Pharmacologist and Immunologist with invaluable experience gained within the science/chemistry industry. Holds a degree in Ph.D. in Pharmacology and Experimental Therapeutics with extensive Quality Control, Testing and Analysis experience including 4.5+ years of laboratory and clinical research experience focusing on cardiovascular and respiratory disease model specific to asthma, COPD, acute lung injury and atherosclerosis in addition to cell culture, molecular biology and immunology expertise.

A resourceful team player that is organized and skilled in providing technical support while demonstrating distinct problem solving and analytical skills. Manages multiple projects and can work independently

Innovative, resourceful and adaptable; generates optimal results with a positive work ethic, energy and desire to continually build on success

• Cell culture: Primary cell isolation/ cell lines and culture including mouse peritoneal macrophages, lung fibroblast, lung smooth muscle cells, human and mouse CD4+ T cells, Jurkat cells, human aortic endothelial cells, human umbilical vein endothelial cells, human pulmonary vein endothelial cells, mouse embryonic fibroblasts, HT-29, HCT cells.
• Murine model of the disease (respiratory and cardiovascular diseases): Different routes of administration of the drugs such as i.n i.v, i.t., i.p.
• s.c.
• Isolation and processing of the murine aorta, broncheoalveolar lavage preparation, serum preparation, organ fixation, tissue processing for RNA and protein analysis, cytospin and differential staining, organ processing and antibody staining for flow cytometry.
• Human Sample Processing: Processing human samples for preparation of serum and analysis and isolation of immune cells using ficoll-paque and using them for in-vitro assays such as skewing and proliferation assays.
• Molecular and bioanalytical techniques: SDS-PAGE, Silver staining, Coomassie Blue staining, Western Blot, ELISA, Transfection, Bradford assay, RNA isolation, Reverse transcription-Polymerase Chain Reaction (RT-PCR), Fluorescence labeling, Live-dead Staining, Immunohistochemistry, Immunofluoroscence, Multiplex assays, comet assay, Non-homologous End Joining Assays Protein Isolation and Purification from bacterial and yeast culture using High Perfomance Liquid Chromatography and affinity chromatography.
• Grant writing practiced throughout the graduate school career; successfully rewarded with AHA predoctoral fellowship.
• Manuscript preparation and Review Leadership/Teaching experience, LSU Health Sciences Center Successfully organized graduate school colloquium for LSUHSC-Department of Pharmacology by inviting a reputed speaker to the department and scheduling different events throughout the colloquium Successfully hosted LSUHSC-Graduate Research day for oral presentations.
• Successfully mentored Sci-Fly High School and Undergraduate students organized by Southern Louisiana Chapter-Association for Women in Science Lectured LSUHSC-Nursing Student Pharmacology Research Support American Heart Association- GSA Winter 2014 Predoctoral Fellowship (07/01/14- 07/01/16) Award amount: $52000 Kusma Pyakurel (Prinicipal Investigator) The goal is to study the role of DNA-PK in inflammation independent of DNA-damage repair in atherosclerosis.
• University of Alabama at Birmingham 2nd annual Metabolomics workshop- Graduate student fellowship (06/02/14-06/05/14) Award amount: $1200.

• ACHIEVEMENTS 2015: 'Best Poster Award' 3rd position, National Conference at Experimental Biology Meeting 2014: Fellowship from American Heart Association (worth $54000) 2013: Fellowship from University of Alabama at Birmingham Metabolomics workshop ($1500) Multiple national conference presentation presentations and publications within a short span of time (Attached)