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Graduate Research Assistant Resume Example

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GRADUATE RESEARCH ASSISTANT
Summary
I have expertise in methods related to microbial DNA recombinant technologies, qPCR, PCR, GFP reporter expression assays, cell culture methods, animal models to study human diseases, and biochemical assays (Western, SDS-PAGE, Gel Shift assay, etc.).  I have expertise in methods related to microbial DNA recombinant technologies, qPCR, PCR, GFP reporter expression assays, cell culture methods, animal models to study human diseases, and biochemical assays (Western, SDS-PAGE, Gel Shift assay, etc.). High-performing researcher able to long hours to achieve outstanding research results.
Highlights
  • DNA isolation expert
  • Microbiological techniques
  • Biochemistry & Mol. Biology
  • Expert in PCR & RT-PCR
  • Cell and tissue culture
  • Immunolabeling fluorescence microscopy
  • Molecular Cloning
  • DNA Sequencing
  • Microbial Genetics
  • Mouse Models of human diseases
  • Teaching and Mentoring
Accomplishments
  • Established expertise in the field of microbial genetics through completion of a 2015 summer course in Advanced Bacterial Genetics at Cold Spring Harbor National Laboratory.
  • Strategically increased productivity in the Hughes laboratory by improving protocols for site directed targeted mutagenesis.
  • Completed Master's Thesis in Microbial Genetics in the laboratory of Dr. Kelly T. Hughes in the genetic regulation of the Bacterial Flagellum in Salmonella.
  • Presented at over 15 different scientific conferences (Oral & Poster Presentations) 
Experience
06/2014 to 12/2016
Graduate Research Assistant University of Utah － San Diego, CA
  • Employed PCR, agarose gel electrophoresis, plasmid preparations, sanger sequencing, restriction enzyme digests, yeast and bacterial transformation, bioinformatics programs, and genetic selections & screens to locate mutants and follow through with experiments.
  • Gained 2 years of experience working with BSL-1 level bacteria (Salmonella and E.coli) to study microbial genetics
  • Presented research to laboratory team on a regular basis.
  • Demonstrated aptitude to learn quickly and work independently. Thought critically about scientific ideas and worked steadily and diligently
  • Taught courses to undergraduate students related to bacterial genetics and microbial diversity. Set up laboratory experiments for students to gain hands-on-experience on how to work independently in a microbial genetics laboratory
09/2010 to 05/2013
Undergraduate Research Assistant San Diego State University － San Diego, CA
  • Worked in the laboratory of Anca Segall on site specific recombination on bacteriophage lambda
  • Received radiation safety certification and 2 year working experience to authorize working with P32 radio-labeled DNA substrates. Used protected clothing at all times, wore safety gloves, used protective sharps containers and disposable waste container to remediate the risk of P32 contamination and used radiation dosimeter to record radiation levels all throughout experiments
  • Generated results to form logical conclusions, modeled experimental data using Matlab software, and investigated enzyme kinetics and molecular mechanism of lambda site-specific recombination.
  • Tested the binding affinities of various DNA repair inhibitors using a 2-AP fluorescence-quenching assay.
  • Presented research at various local, regional, national, and global scientific venues.
  • Familiar with BSL-2 level pathogen labs by working in an the Segall lab that used HeLa cell lines to study the effect of various DNA repair inhibitors on pathogenic clinical isolates of bacteria. Required laboratory coat, gloves, and UV-protective lamb under a closed fume hood to prevent aerosols and accident spills. 
  • Microbiology skills: aseptic and sterile techniques, optical microscopy, bacterial staining, plating methods (streak, spread, pour, replica), enumeration and identification of bacteria, use of biological safety cabinets, media and buffer preparation, water sample analysis by membrane filtration and MPN, food microbiology, diagnostic biochemical tests for enteric bacteria, use of selective media, antibiotic assay (MIC), plaque assay, conjugation 
  • Biochemistry and Cell & Molecular Biology skills: DNA extraction, plasmid DNA preparation, restriction enzyme digests, PCR, Quantitative PCR, DNA radiolabeling, agarose gel electrophoresis, SDS-PAGE, cell fractionation by differential centrifugation, preparation of cells for cryopreservation.
09/2010 to 05/2012
Mentor - Amachi Program Big Brothers Big Sisters of America － San Diego, CA
  • Volunteered in the San Diego County Big Brothers and Big Sisters Amachi Program to mentor children of incarcerated parents.
  • Went on one-on-one outings with my little brother to the San Diego Zoo, the Birch Aquarium, Sea World, art galleries, recreational/sporting events, and research laboratories, while sharing stories of past experiences during my youth
03/2010 to 05/2013
MBRS Scholar NIH SDSU Initiative for Maximizing Student Development － San Diego, CA Applied and was accepted into the MBRS program whose focus is to improve the participation of historically underrepresented students in the sciences and increase the number of underrepresented students who matriculate directly to a Ph.D. program. Received travel awards for scientific meetings & conferences and other unique opportunities to present experimental observations.
Education
2017
Master of Science: Biology University of Utah － Salt Lake City, UT, 84112
2013
Bachelor of Arts: Microbiology San Diego State University － San Diego, CA, USA
Professional Development

  • Presented at over 15 different scientific conferences (Oral & Poster Presentations) 
  • Society for Advancement of Chicanos/Hispanics and Native Americans in Science.
  • Gained advanced bacterial genetics experience in 3 week summer intensive program at Cold Spring Harbor Laboratory in 2015 working with vibrio vulnificus species, e.coli, and staph aureus species. Required to wear protective safety lab coat and gloves 
Skills
  • Autoclaving
  • Gram Staining
  • Strain Construction
  • Aseptic Technique and Pipetting
  • Cell Culturing and Plating
  • PCR
  • Nucleic Acid Isolation
  • Microbial Genetics
  • Quantitative PCR
  • Fluorescent Microscopy
  • Biochemistry and Molecular Biology
  • Mouse Modeling of ExPEC 
  • Oral Gavage Feeding
  • Enterocyte isolation
  • Teaching
Awards
Certificate of Recognition, Cold Spring Harbor Laboratory
Dr. Diarmaid Hughes, Dr. Fitnat Yildiz, and Dr. Beth Lazazzera
  • For participation and completion of the summer intensive Advanced Bacterial Genetics Course
 
Certificate of Recognition, SDSU MBRS/IMSD Program
Dr. Cathie Atkins and Dr. William Tong
  • For contributions to undergraduate scholarly research at the 2012 Undergraduate Research Symposium (URS).
 
Certificate of Appreciation, SDSU Junior ROTC STEM CAMP
Dr. Bruce Westermo, Professor & Graduate Advisor, Department of Civil and Environmental Engineering
  • Supported and contributed to the SDSU JUNIOR ROTC STEM CAMP. I toured High School students from low income communities through Dr. Anca Segall's laboratory and discussed my research and about the fundamentals of SDS-Polyacrylamide Gel Electrophoresis (SDS- PAGE) and the polymerase chain reaction (PCR).
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Resume Overview

Companies Worked For:

  • University of Utah
  • San Diego State University
  • Big Brothers Big Sisters of America
  • NIH SDSU Initiative for Maximizing Student Development

School Attended

  • University of Utah
  • San Diego State University

Job Titles Held:

  • Graduate Research Assistant
  • Undergraduate Research Assistant
  • Mentor - Amachi Program
  • MBRS Scholar

Degrees

  • Master of Science : Biology
    Bachelor of Arts : Microbiology

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