Brings 17 years of applied research experience as well as administrative expertise in managing a scientific research group. Detail-oriented and proactive at cutting costs without compromising productivity and efficiency of the research laboratory.
Core Qualifications
Data collection and analysis
Able to work independently and as a member of a team
Analytical thinker
Budget conscious
Creative problem solver
Comfortable with diverse populations
Exceptional organizational skills
Excellent relationship building skills
Experience
Biologist and Technical Laboratory Manager, 01/2002 - Current Basf – Shakopee, MN,
Maintenance of supplies and chemical inventory Maintaining laboratory equipment and negotiates contractual service agreements for all high end scientific equipment.
Able to locate instructions and other guide material to problem solve.
Manages all of the ordering of necessary research supplies for the laboratory.
Decreased costs by negotiating cost saving quotes with supply vendors.
Independently plans work in order to meet priority requirements.
Works with Principle Investigator in designing and developing new protocols.
Data collection and analysis using various software programs Teaches scientific collaborators the use of delicate equipment as well as aiding them in their research.
Provides guidance and aid in maintaining central facilities that are needed for research.
Provide instruction and training for junior researchers as well as students.
Guiding all new researchers through the NIH training procedures Scientific Experience Proficient in biochemistry, cell and molecular biology techniques.
Protein extraction from mammalian cells, yeast as well as animal tissue.
Maintenance of cell lines.
Mouse husbandry.
Quantitative protein assays.
FPLC Using various conditions and treatments to manipulate or find proteins.
Western blotting.
Prepare neuronal cultures to analyze proteins either by immunocytochemistry or by western blotting.
DNA preps for transfections and transformations in mammalian cells.
Mutagenesis and subcloning using cloning vectors Independently designs experiments and researches new approaches to the study Detailed and orderly records kept for all experimental studies.
Able to do minor repairs on small lab equipment.
Research Technician II, 01/1997 - 01/2002 County Of Bexar – Paul Elizondo Tower - 101 West Nueva San Antonio, TX,
Independently managed more than 20 separate genetically engineered mouse colonies.
Executed laboratory experiments using molecular biology, southern blot analysis, dot blot analysis, DNA subcloning as well as isolation and synthesis of radioactive DNA probes.
Western blot analysis on mouse tissues.
Micro dissections and collection of tissue for histological analysis and familiar with collection of 8-cell stage embryos and sperm for cryopreservation.
Behavioral analysis on studies of mice drug consumption, dependence, and physiological effects.
Injection of pharmacological agents into mice, blood drawing and initiating serum analysis.
Assisted Professors Richard Palmiter and Robert Braun part-time in transgenic research which included hormonal manipulation of mice for super-ovulation and blastocyst production.
Maintained computer database of mouse genotype information.
Animal Care Technician, 01/1996 - 01/1997 The Jackson Laboratory – City, STATE,
Maintained more than five colonies of mice simultaneously in large production facility.
Conducted all work according to specific pathogen free (SPF) environment protocol.
Assessed deviations in phenotypes and general health status of mice.
Education
Bachelor of Arts: , Expected in December 1995 - University of Southern Maine - Portland, Maine GPA:
Professional Affiliations
Publications
Renvoisé B, Claire J, Singh R, Bakowska JC, Blackstone C. Spg20/ mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling. Hum Mol Genet. 2012; 21:3604-18 Chang CR, Manlandro CM, Arnoult D, Claire J, Posey AE, Hill RB, Blackstone. (2010) A lethal de novo mutation in the middle domain of the dynamin-related GTPase Drp1 impairs higher order assembly and mitochondrial division. J Biol Chem. 2010; 285:32494-503 Soderblom C, Claire J, Jupille H, Blackstone C, Shupliakov O, Hanna MC. Targeted disruption of the Mast syndrome gene SPG21 in mice impairs hind limb function and alters axon branching in cultured cortical neurons. Neurogenetics. 2010; 11:369-78. FigueroaRomero C, IñiguezLluhí JA, Claire J, Chang CR, Arnoult D, Keller PJ, Hong Y, Blackstone C, Feldman EL. SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle. FASEB J. 2009; 23:3917-27 Rismanchi N, Soderblom C, Claire J, Zhu PP, Blackstone C. Atlastin GTPases are required for Golgi apparatus and ER morphogenesis. Hum Mol Genet. 2008; 17:1591-604 Meijer IA, Dion P, Laurent S, Dupré N, Brais B, Levert A, Puymirat J, Rioux MF, Sylvain M, Zhu PP, Soderblom C, Claire J, Blackstone C, Rouleau GA. (2007). Characterization of a novel SPG3A deletion in a French-Canadian family. Ann Neurol. 2007; 61:599-603. Zhu PP, Soderblom C, Tao-Cheng JH, Claire J, Blackstone C. SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development. Hum Mol Genet. 2006 15:1343-53 Zhu PP, Patterson A, Claire J, Seeburg DP, Sheng M, Blackstone C. (2004). Intra- and intermolecular domain interactions of the C-terminal GTPase effector domain of the multimeric dynamin-like GTPase Drp1. J Biol Chem. 2004; 279:35967-74 Zhu PP, Patterson A, Lavoie B, Claire J, Shoeb M, Patel R, Blackstone C. (2003). Cellular localization, oligomerization, and membrane association of the hereditary spastic paraplegia 3A (SPG3A) protein atlastin. J Biol Chem. 2003; 278:49063-71 Miller DW, Ahmad R, Hague S, Baptista MJ, Canet-Aviles R, McLendon C, Carter DM, Zhu PP, Claire J, Chandran J, Klinefelter GR, Blackstone C, Cookson MR. (2003). L166P mutant DJ-1, causative for recessive Parkinson's disease, is degraded through the ubiquitin-proteasome system. J Biol Chem. 2003; 278:36588-95 Thiele TE, Willis B, Claire J, Reynolds JG, Bernstein IL, McKnight GS. (2000). High ethanol consumption and low sensitivity to ethanol-induced sedation in protein kinase A-mutant mice. Journal of Neuroscience. 2000; 20:RC75.
Skills
Data collection, database, designing, drawing, instruction, inventory, Works, negotiating, protocols, repairs, research, Scientific, SPF, Western blotting
Additional Information
Additional Information
NINDS Group Merit Award 2013
NINDS Individual Award 2014
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