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Associate Research Scientist, Department of Anesthesiology Resume Example

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ASSOCIATE RESEARCH SCIENTIST, DEPARTMENT OF ANESTHESIOLOGY
Profile
Research scientist with strong background in neuroscience and extensive experience in electrophysiology, biochemistry and cell biology. Having broad knowledge base in the research of synaptic transmission, homeostasis and neuropathic pain. Extensive experience of collaboration with major pharmaceutical companies to bridge the basic research and clinical application. Extensive experience in research project initiation and management, in vitro assay design and implementation, data analysis, scientific writing and presentation. Familiar with GLP and regulatory guidelines (FDA/ICH) regarding pharmaceutical development. Strong leadership and communication skills in task coordination across functional platforms. Self-motivated, proactive and dedicated team player.
Skills
  • Neuroscience (pain, synaptic transmission, homeostasis) Technical Expertise
  • Electrophysiology techniques: whole cell/perforated patch clamp and extracellular recording in brain/spinal cord slices and cell cultures
  • Ion selective microelectrodes: Recording extracellular changes in K+, Ca2+, pH or other ion concentration in brain/spinal cord slice preparations
  • Stereotaxic microinjection of virus: Injection of recombinant virus that express fluorescent proteins to retrogradely or antereoregradely label the connecting neurons to explore the circuitry of brain and spinal cord
  • Imaging techniques: Calcium/chloride imaging in slice preparations and confocal/two photon microscopy.
  • Rodent Injury models and animal handling: spinal cord injury and peripheral nerve injury in rats and mice, including spared nerve injury and post-operative animal handling after spinal cord injury.
  • Patent specification writing: Writing biotechnology patent specifications and responses to USPTO
Professional Experience
Associate Research Scientist, Department of Anesthesiology08/2012 to CurrentColumbia University Medical CenterNew York, NY
  • Collaborated with Bristol-Myers Squibb to identify the mechanism of action of novel compounds on neuropathic pain
  • Led a research project to uncover the functional role of inhibitory interneurons in the dorsal horn of the spinal cord using electrophysiology, immunocytochemical staining and fluorescence microscopy
  • Investigated the circuitry of projection neurons in the dorsal horn using stereotaxic microinjection of recombinant virus in brainstem to anterogradely or retrogradely label subpopulations of neurons in brain and spinal cord.
  • Reviewed manuscripts submitted to professional journals, such as Journal of Neuroscience, British Journal of Pharmacology.
Postdoctoral Research Scientist08/2003 to 07/2012Department of Physiology and Cellular Biophysics, Columbia University Medical CenterNew York, NY
  • Collaborated with Wyeth Pharmaceutical to identify the differential expression of functional NMDA receptor subunits in subpopulations of dorsal horn neurons.
  • Developed novel techniques for spinal cord slicing with intact roots attached, which was subsequently and routinely used by many other labs doing spinal cord research.
  • Wrote grant proposals as the principle investigators to apply for research funding, including NIH R01, R21, Wings for Life, New York State Spinal Cord Injury Research Program.
  • Advised and supervised postdoctors, graduate students and undergraduate students.
  • Designed and developed a novel technique to identify functional presynaptic receptors expressed at the terminal of primary afferent fibers within the dorsal horn of the spinal cord
  • Trained undergraduate students to conduct scientific research projects.
Patent Engineer07/1993 to 07/1995Top Team International Patent and Trademark OfficeTaipei
  • Wrote English patent specifications and filed US/European/China/Taiwan patent applications
  • Worked in partnership with our lawyers to investigate patent infringements and discovered counterfeit goods for our clients.
Second Lieutenant07/1991 to 06/1993Army Aviation Headquarters
  • Led a team to manage the helicopter part supply and technical manuals in our Aviation Maintenance & Repair Unit
  • Lecturing on core courses for military academy students, including "Helicopter Parts Management" and "Technical manual management"
Education and Training
Ph.D.: Physiology & Neuroscience2000NYU Medical CenterNew York, NY, US1995-2000 Ph.D. Department of Physiology & Neuroscience, NYU Medical Center, New York, NY
M.S.: Life Science1991National Tsing Hua University1989-1991 M.S. Institute of Life Science, National Tsing Hua University, Hsinchu, Taiwan
B.S.: Physics1989National Tsing Hua University1985-1989 B.S. Department of Physics, National Tsing Hua University, Hsinchu, Taiwan Book "The role of pH in Brain Function: Function and Characterization of Extracellular Carbonic Anhydrase in Brain" by Dr. Chi-Kun Tong, publisher: VDM Verlag, sold by Amazon.com
Military Experience
Second Lieutenant07/1991 to 06/1993ArmySecond Lieutenant, Army Aviation Headquarters, Tainan, Taiwa 7/1991 - 6/1993 Led a team to manage the helicopter part supply and technical manuals in our Aviation Maintenance & Repair Unit Lecturing on core courses for military academy students, including "Helicopter Parts Management" and "Technical manual management"
Certifications
ATP
Publications
containing NMDA receptors within the superficial dorsal horn activated following primary afferent stimulation. 34(33): (corresponding author) Synaptic GluN2A and GluN2B and postsynaptic inhibitory control in the spinal cord dorsal horn. Ann N Y Acad Sci. 2013 Mar;1279: (2010) NR2 subunits and NMDA receptors on lamina II inhibitory and excitatory interneurons of the mouse dorsal horn. Mol Pain. 6:26 Bardoni R, Takazawa T, Tong CK, Choudhury P, Scherrer G, Macdermott AB Functional identification of NR2 subunits contributing to NMDA receptors on substance P receptor-expressing dorsal horn neurons, Mol. Pain, 44 (corresponding author) (2006) Both Ca2+ permeable and impermeable AMPA receptors contribute to primary synaptic drive onto rat dorsal horn neurons J Physiology, 575(Pt 1): (corresponding author) Tong CK, Chen K, Chesler M (2006) Kinetics of activity-evoked pH transients and extracellular pH buffering in rat hippocampal slices, J Neurophysiology, 95(6):3686-97 Tong CK, Edward Kaftan, MacDermott AB Presynaptic NMDA receptors modulate glutamate release from primary sensory neurons in rat spinal cord dorsal horn, J. Neuroscience, 24(11): (2003), Localization and function of ATP and GABAA receptors expressed by nociceptors and other postnatal sensory neurons in rat, J Physiology, 549 (Pt 1):131-42. *Lee CJ, *Bardoni R, *Tong CK, Engelman HS, Magherini PC, and MacDermott AB (2002), Functional expression of AMPA receptors on central terminals of rat dorsal root ganglion neurons and presynaptic inhibition of glutamate release, Neuron, 35(1):135-46 Bardoni R, Torsney C, Tong CK, Prandini M, MacDermott AB carbonic anhydrase (CA) activity in brain is attributable to membrane-bound CA type IV, , 20(22) Tong CK, Brion L P, Suarez C, Chesler M Chesler M , of spreading depression by changes in extracellular p 84(5) H, J. Neurophysiology, , Neuroscience, 86(1):39-51 Tong CK, Pan MP, Chang YC (1992) Characterization of L-glutamate and kainate binding sites in the brain of a freshwater fish, Telapilia monsanbica Tong CK, Cammer W, Chesler M
Additional Information
Writing biotechnology patent specifications and responses to USPTO
Skills
Associate, Proposals, Neuroscience, Publisher, Circuitry, Electrophysiology, Journal, Pharmacology, Staining, Toxicology, Clients, Engineer, Patent, Patent Applications, Patent Infringements, Aviation, Aviation Maintenance, General Aviation, Maintenance, Life Science, Acad, Adme, Animal Handling, Antibodies, Auto Cad, Biochemistry, Biotechnology, Cell Biology, Data Analysis, Fda, Glp, Ich, Imaging, Ion, Marketing Analysis, Mouse, Pharmaceutical Development, Proactive, Self Motivated, Team Player, Vitro
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Resume Overview

Companies Worked For:

  • Columbia University Medical Center
  • Department of Physiology and Cellular Biophysics, Columbia University Medical Center
  • Top Team International Patent and Trademark Office
  • Army Aviation Headquarters
  • Army

School Attended

  • NYU Medical Center
  • National Tsing Hua University

Job Titles Held:

  • Associate Research Scientist, Department of Anesthesiology
  • Postdoctoral Research Scientist
  • Patent Engineer
  • Second Lieutenant

Degrees

  • Ph.D. : Physiology & Neuroscience 2000
    M.S. : Life Science 1991
    B.S. : Physics 1989

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