Expert in human sample examinations Morphological examination in blood, urine and body fluids, metabolic analysis by biochemistry, type of blood test, identify of bacteria species, isolation of lymphocyte for immune test.
Expert in pathological techniques
Handled embedding, and microtomy of both frozen and paraffin sections from human tissues, staining techniques by histochemistry, immunohistochemistry, and immunofluorescence.
Skilled in protein extraction and analysis Protein extraction and purification, analyzed by immunoprecipitation, Western Blot, and Blue-Native Gel Electrophoresis.
Expert in DNA extract and analysis
Extract DNA from cells and mitochondria, digestion, and DNA amplification by the polymerase chain reaction (PCR), gene cloning techniques, purification of yeast genomic DNA fragment and bacterial plasmid DNA, Southern Blot, replication 2-D gel, and Pulsed-field Gel Electrophoresis (PFGE).
Proficient in other experimental techniques
Isolation of both lymphocyte and mitochondria by gradient centrifugation, cell cultures, in vitro transfection, gene cloning, and ELISA.
Experienced in animal handling
Animal care (mouse and rabbit), making polyclonal antibody, and mouse breeding to keep the line.
Operating robotics equipment, maintenance of a large number of yeast strains and of lab equipment, ordering of supplies, data analysis using software included Excel, PowerPoint, Photoshop, and reporting, training summer students.
Rutgers-New Jersey Medical SchoolResearch Teaching Specialist
Investigated the role of the Pif1 DNA helicase in maintenance of mitochondrial DNA in yeast using cellular and molecular techniques, such as chronological lifespan, gene cloning, isolation and purification of mitochondria DNA, PCR, pulse field gel electrophoresis (PFGE), Southern blot, 2-D gel, immunoprecipitation, Western blot, transformation of yeast with plasmid DNA, purification of plasmid DNA, analysis of plasmid DNA by restriction enzyme digestion.
Banting and Best Institute University of TorontoResearch Specialist
Studied on the functions of gene using molecular biology by purification of DNA, digestion of DNA by restriction enzyme, and Synthetic Genetic Array analysis by robotics for screening of conventional yeast two-hybrid.
Duty for construction, characterization and management of a large number of yeast strains, programming, troubles shooting, records keeping, and assisting with data analysis.
Studied on detergents induced cell proliferation in human embryo cells using cell culture, cell toxicity by MTT test, Lucifer yellow staining, and protein expression by Western blot.
Changzheng Hospital, Second Military Medical University of Shanghai, P. R. ChinaMedical Technologist
Performance routine examinations of human samples included blood, urine, and body fluids analyzed by biochemistry, immunology, immunochemistry, microbiology, blood bank, and pathology using following techniques, such as ELISA, immunoassays, embedding, and microtomy of frozen and paraffin sections from human and mouse tissues, staining techniques (histochemistry and immunofluorescence), and microscopy.
Also, I helped scientists to make polyclonal antibody for their research projects.
Second Medical Military University of ShanghaiP. R., ChinaBA: Medical ScienceMedical Science
Nanjing Military Medical SchoolP. R., ChinaAssociate B.Sc: Laboratory MedicineLaboratory Medicine
Department of Cell Biology and Molecular Medicine
JS. Schneider, X. Cheng, Q. Zhao, C. Underbayev, JP. Gonzalez, ES. Raveche, D. Fraiderich, AS. Ivessa. Reversible Mitochondrial DNA Accumulation in Nuclei of Pluripotent Stem Cells. Stem Cells Dev. (2014) Aug 4. [Epub ahead of print] X. Cheng and AS. Ivessa. Accumulation of linear mitochondrial DNA fragments in the nucleus shortens the chronological life span of yeast. Eur. J. Cell Biol. 91(10): 782-788 (2012). X. Cheng and AS. Ivessa. Association of the yeast DNA helicase Pif1p with mitochondrial membranes and mitochondrial NDA. Eur. J. Cell Biol. 89(10): 742-747 (2010). X. Cheng and AS. Ivessa. The migration of mitochondrial DNA fragments to the nucleus affects the chronological aging process of Saccharomyces cerevisiae. Aging Cell. 9(5): 919-923 (2010). X. Cheng, Yong Qin, and AS. Ivessa. Loss of mitochondrial DNA under genotoxic stress conditions in the absence of the yeast DNA helicase Pif1p occurs independently of the DNA helicase Rrm3p. Mol Genet Genomics 281(6):635-45 (2009). X. Cheng, S. Dunaways, and AS. Ivessa. The role of Pif1p, a DNA helicase in Saccharomyces cerevisiae, in maintaining mitochondrial. Mitochondrion 7(3):211-22 (2007). AP. Davierwala, J. Haynes, Z. Li, RL. Brost, MD. Robinson, L. Yu, S. Mnaineh, H. Ding, H. Zhu, Y. Chen, X. Cheng, GW. Brown, C. Boone, BJ. Andrews and TR. Hughes. The synthetic genetic interaction spectrum of essential genes. Nat Genet. 37:1147-52 (2005). TA. Tong, G. Lesage, GD. Bader, H. Ding, H. Xu, X. Xin, J. Young, GF. Berriz, RL. Brost, M. Chang, Y. Chen, X. Cheng, G. Chua, H. Friesen, DS. Goldberg, J. Haynes, C. Humphries, G. He, S. Hussein, L. Ke, N. Krogan, Z. Li, JN. Levinson, H. Lu, P. Menard, C. Munyana, AB. Parsons, O. Ryan, R. Tonikian, T. Roberts, AM. Sdicu, J. Shapiro, B. Sheikh, B. Suter, SL. Wong, LV. Zhang, H. Zhu, CG. Burd, S. Munro, C. Sander, J. Rine, J. Greenblatt, M. Peter, A. Bretscher, G. Bell, FP. Roth, GW Brown, B. Andrews, H. Bussey and C. Boone. Global mapping of the yeast genetic interaction network. Science 303: 808-813 (2004).
Photoshop, data analysis, ELISA, Excel, PowerPoint, PCR, programming, reporting, research, robotics, type