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Research Associate Resume Example

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RESEARCH ASSOCIATE
Summary
Research Scientist
A highly motivated, collaborative and creative scientist with extensive academic and practical experience. Expertise in major molecular and cellular biology research techniques
Highlights
  • Cancer research
  • Assay development
  • Angiogenesis
  • Protein analysis
  • Genetic expression analysis
  • In Vivo tumor implants
  • Mammalian cell culture
  • Endothelial cell isolation
  • IHC, IF, PCR, RT-PCR , RT-qPCR
  • Molecular cloning
Accomplishments
  • Established the role of FOXO1 and FOXO3 as possible tumor suppressor molecules in vascular endothelial biology and identified novel molecular interactions between FOXO1 and its downstream target genes Sprouty2 and PBX1.
  • MAX PLANCK INSTITUTE, BAD NAUHEIM, GERMANY 2005 - 2009 PH.D.
  • Student Conducted research on the Molecular mechanism of the transcriptional Co-activator gene VITO1 in skeletal muscle gene regulation.
  • Performed Yeast two hybrid (Y2H) screens to identify novel interacting partners for VITO1 using heart and skeletal muscle cDNA library.
  • Substantiated the interaction of VITO1 with sarcomeric Z - disc proteins (IP, GST pull down).
  • Compared expression and subcellular localization of VITO1 and its novel interacting partners in primary chicken myocytes and mouse cardiomyocytes (fluorescence and confocal microscopy).
  • Interpreted the dual role of VITO1 as a transcriptional co-activator and a stress signaling molecule at the Z-discs of the sarcomere through its nucleo-cytoplasmic shuttling phenomenon.
  • Significance: Established a novel correlation between the muscle specific transcriptional co-activator VITO1 and the Z-disc proteins telethonin and myozenin that might play an important role in the formation of slow muscle fibers.
  • Martin-Luther University, Halle, Germany 2003 - 2005 Research Student Interplay of the membrane protein p0071 in regulating the cytoskeleton and mediating cell-cell contact.
  • Mapping binding site of p0071 membrane protein to cadherins and Formins (FHOD1) using Y2H.
  • Generated luciferase reporter, GTPase and fluorescence assays to study binding of p0071 to cadherins and FHOD.
  • Performed p0071 protein expression and purification using Fast protein liquid chromatography (FPLC) to identify protein complexes and further analyze by mass spectrometry Reutlingen University, Germany (M.Sc in Biobased Materials) 2000 - 2002 Isolation and Identification of glycans from glycoprotein using chromatographic techniques.
  • Applied enzymatic and chemical methods to cleave glycans from glycoprotein.
  • Utilized High performance liquid chromatography (HPLC) and capillary electrophoresis to identify the glycans in ovalbumin and estimate molecular weight using MALDI-TOF.
  • Compared and analyzed the anti-inflammatory compound boswellic acid present in natural Olibanum resin with tablets made from them by a pharmaceutical company using chromatographic techniques.
Experience
02/2014 to Current
Research AssociateHampton University - Hampton , VA

Project 1: Investigate the molecular mechanism by which SPRY2 and PBX1 regulates vascular tumors

Project 2: Functional characterization of primary human liver Angiosarcoma-derived endothelial cells.

  • Performed microarray gene expression analysis and employed DAVID 6.7 functional annotation tool combined with GeneMANIA Cytoscape plugin to do pathway analysis of differentially regulated genes upon SPRY2 and PBX1 knockdown in primary angiosarcoma cells
  • Established sprouty2 as a pivotal mediator of PI3K/ AKT and MAPK kinase signaling pathway.
  • Validate novel target genes of PBX1 and SPRY2 and study their genetic expression, function and regulation in vascular tumor endothelial cells (qPCR, western blots, immunohistochemistry, shRNA transduction, live cell imaging in confocal microscope and FACS).
  • Isolate endothelial cells from patient tissues, characterize and study the effects of Rapamycin and MAPK inhibitors as potential therapeutic drug targets.
  • Mentoring graduate students, scientific writing, posters and presentations.

Significance: Demonstrated the important growth regulatory roles of FOXO1/Sprouty2 and FOXO1/PBX1 pathway in vascular tumors that reveal potential new therapeutic strategies for these tumors by modulating and targeting this pathway.

01/2010 to 01/2014
Postdoctoral FellowMassachusetts General Hospital - Everett , MA
  • Explored the function of Forkhead transcription factors FOXO1 and FOXO3 in vascular tumor growth and studied the differential roles of AKT1 and AKT3 in vascular tumor development.
  • Applied various molecular and cellular biology techniques (wound healing, apoptosis, proliferation, chromatin Immunoprecipitation, lentiviral knockdown, Neon transfection, qPCR, cloning) to study the function of FOXO1 and FOXO3 in vascular tumor endothelial cells.
  • Screened for drug targets that specifically blocks AKT1 isoform in vascular tumor endothelial cells by high-throughput screening using Tecan Freedom Evo Robotics System (CDD core, BCM).
  • Directed mammalian cell culture, use of primary angiosarcoma and other vascular cell tumor lines.
  • Mentored MD residential candidates, technicians and students on a daily basis.
  • Identify, prioritize, expand collaborations and generate scientific data for research grants.
Education
Ph.D: BiologyMax Planck Institute for Heart and LungGermanyBiology
M.Sc. degree: Bio based MaterialsReutlingen UniversityGermanyBio based Materials
B.Sc. degree: ChemistryMadras UniversityIndiaChemistry
Publications
SELECT PEER-REVIEWED PUBLICATIONS "Akt1 and Akt3 exert opposing roles in the regulation of vascular tumor growth." Cancer Research 2015, 75(1):40-50. Phung TL, Du W, Xue Q, Ayyaswamy S, Gerald D, Antonello Z, Nhek S, Perruzzi CA, Acevedo I, Ramanna-Valmiki R, Rodriguez-Waitkus P, Enayati L, Hochman ML, Lev D, Geeganage S & Benjamin LE "FOXO3 inhibits vascular tumor growth" Ayyaswamy S, Du W, Enayati L, Mejbel H, Haws A, Rosales C, Abid R and Phung TL (manuscript ready for submission) "FOXO1 regulates vascular tumor growth through Sprouty-2" Ayyaswamy S, Du W, Anderson C, Haider M, Enayati L and Phung TL (manuscript ready for submission) "FOXO1negatively regulates PBX1 to inhibit vascular tumor growth" Ayyaswamy S, Du W, Anderson C, Haider M and Phung TL (manuscript in preparation). "A novel role for the VITO1 (VGLL2) co-activator in skeletal muscle gene regulation" Ayyaswamy S, Guenther S, Kruger M and Braun T (manuscript in preparation)
Skills
academic, biology, Cancer, cell culture, Functional, grants, imaging, mediator, Mentoring, novel, pathway, PBX1, PCR, posters, presentations, RESEARCH, Robotics, RT-PCR, scientific, scientific writing, transcription
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Resume Overview

School Attended

  • Max Planck Institute for Heart and Lung
  • Reutlingen University
  • Madras University

Job Titles Held:

  • Research Associate
  • Postdoctoral Fellow

Degrees

  • Ph.D : Biology
    M.Sc. degree : Bio based Materials
    B.Sc. degree : Chemistry

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