Research Scientist with 10 years of laboratory research and managing experience. Accomplished in generating and applying new molecular biology protocols and technologies to advance scientific goals. Talented individual who can produce results whether working in cells, live animals, or cell free environments. Expert knowledge of cell biology, molecular biology, and genetics. Experienced researcher who has strong skills in organizing, writing, editing, and publishing research papers. Skilled communicator who is at ease speaking to small or large audiences and people with varying backgrounds. A meticulous hard working individual who is an ardent professional no matter what the situation and can work individually or in a team orientated setting to meet deadline goals.
Cell Culture: Transient transfections, growth of transformed mouse Sertoli cells, and growth of primary rat and mouse Sertoli cells and germ cells.
Cell Isolation: FACS, MACS
Animal Models: Organized and maintained wild type, transgenerational, and transgenic mouse colonies, performed germ cell isolation, primary Sertoli cell isolation, and in vivo experiments using mouse and rat model system.
Microscopy: Immunohistochemistry, Immunofluorescence, and Confocal
Computer: Proficient in using Microsoft office products, Adobe Photoshop and Illustrator. Also, proficient in using and learning programs necessary for large scale array analysis.
DNA: Subcloning, genotyping PCR, site-directed mutagenesis,
Methylated DNA Immunoprecipitation, Next Generation Sequencing, library
construction, and DNA promotor array analysis.
RNA: RT-PCR, Real time PCR, RNA purification, RNA expression microarray analysis.
Protein: SDS PAGE, Western Blots, Immunoprecipitation.
Discovered and published that exposure of pregnant female mice to di-2-ethylhexyl phthalate induced testicular abnormalities that were transgenerational in nature in both inbred and outbred mice.
Using next generation sequencing and promoter tilling arrays I have
identified 9 potential genes that have methlyation changes in their DNA
that may be leading to the observed di-2-ethyhexyl phthalate induced
Identified and published that exposure to di-2-ethylhexyl phthalate impairs testicular stem cell proliferation and differentiation.
Identified and published several pathways that are regulated by retinoids in the Sertoli cell.
Identified and published that loss of the retinoic acid receptor in the testis leads to inhibition of Sertoli cell and germ cell function, and specifically that testicular stem cells are impaired.
Postdoctoral Research Associate/Lab Manager09/2004 to 03/2016Washington State UniversityPullman, WA
Managed the everyday operations of the laboratory; was responsible for purchasing supplies, maintenance of equipment, directing undergraduate student employees, and interacting with heads of laboratory facilities.
Directed the everyday workings of the laboratories mouse colony, which included directing two technician's and undergrad student employee daily activities.
Managed the Center for Reproductive Biology Histology Core group, which included directing undergraduate student employees, interacting with individual clients, and working with Histology Laboratory employees to deal with issues that arose.
Mentored and managed eight undergraduate student projects from design to completion, leading to awards in undergraduate research and graduation with honors.
Mentored and guided six graduate students in their development as scientists and eventual graduation with masters or doctoral degrees.
Designed and executed a project using Next Generation Sequencing and promoter tilling arrays to identify changes in DNA methylation status after exposure to the environmental toxin di-2-ethylhexyl phthalate.
Designed and executed a project to investigate the transgenerational status of inbreed mice to exposure of the environmental toxin di-2-ethylhexyl phthalate.
Designed and executed a project to investigate the transcriptome expression in rat Sertoli cells after exposure to retinoic acid and follicle stimulating hormone.
Collaborated with researchers at the University of Virginia to determine if di-2-ethylhexyl phthalate could affect the stress response of mice in multiple generations.
Investigated the potential of di-2-ethylhexyl phthalate to induce testicular abnormalities and if those testicular abnormalities were transgenerational in nature.
Collaborated with researchers at Texas AM to identify immune system genes expressed in mouse Sertoli cells that could potentially be responsible for making the testis an immune privileged site.
Investigated the global transcription expression of genes in the testis of vitamin A deficient rats after exposure to retinol.
Research Assistant05/2000 to 08/2004Washington State UniversityPullman, WA
Investigated the role retinoic acid receptor alpha plays in male germ cell development.
Mentored and managed four undergraduate projects from design to completion, leading to awards in undergraduate research and graduation with honors.
Undergraduate Research08/1998 to 07/1999California State University at SonomaRohnert Park, CA
Designed and executed research project to create a new immunology teaching laboratory to instruct undergraduate students how vaccines functioned.
Education and Training
Ph.D: Genetics and Cell Biology2004Washington State UniversityPullman, WAGenetics and Cell Biology
Bachelor of Science: Cell Biology1999California State University at SonomaRohnert Park, CACell Biology
Quinnies KM, Doyle TJ, Kim KH, Rissman EF. Transgenerational Effects of Di-(2-Ethylhexyl) Phthalate (DEHP) on Stress Hormones and Behavior. Endocrinology. 2015 Sep;156(9):3077-83.
Doyle TJ, Bowman JL, Windell VL, McLean DJ, Kim KH. Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice. Biol Reprod. 2013 May 2;88(5):112.
Doyle TJ, Kaur G, Putrevu SM, Dyson EL, Dyson M, McCunniff WT, Pasham MR, Kim KH, Dufour JM. Immunoprotective properties of primary Sertoli cells in mice: potential functional pathways that confer immune privilege. Biol Reprod. 2012 Jan 10;86(1):1-14.
Zhu L, Doyle TJ, Kim KH. Retinoic acid modulates the subcellular localization of small ubiquitin-related modifier-2/3 (SUMO-2/3) in the testis. J Androl. 2010 Jul-Aug;31(4):406-18.
Doyle TJ, Oudes AJ, Kim KH. Temporal profiling of rat transcriptomes in retinol-replenished vitamin A-deficient testis. Syst Biol Reprod Med. 2009 Aug;55(4):145-63.
Doyle TJ, Braun KW, McLean DJ, Wright RW, Griswold MD, Kim KH. Potential Functions of Retinoic Acid Receptor A (RARA) in Sertoli cells and Germ Cells During Spermatogenesis. Ann NY Acad Sci. 2007; 1120: 114-130
Das P, Doyle TJ, Liu D, Kochar J, Kim KH, Rogers MB. Retinoic acid regulation of eye and testis-specific transcripts within a complex locus. Mech Dev. 2007 Feb; 124(2):137-45
Honors and Awards 2013: Manuscript: Transgenerational effects of di-(2-ethylhexyl) phthalate on testicular germ cell associations and spermatogonial stem cells in mice was showcased on Faculty F100 website. 2011: Washington State University School of Molecular Biosciences Post - Doctoral Researcher of the year. Travel Award from XXI North American Testis Workshop. Washington State University Center for Reproduction Travel Award. 2003: School of Molecular Biosciences Travel Award. Washington State University Graduate School Travel Grant 1996 to 1999: Dean's List
acid, Adobe Photoshop, alpha, Biology, Cell Culture, clients, directing, DNA, Endocrinology, experiments, Illustrator, immunology, MACS, Microsoft office products, Next, PAGE, PCR, purchasing, Real time, research, RT-PCR, teaching, technician, transcription, type, vaccines, Written