Substantial research experience 13 years of experience in plant molecular biology (Arabidopsis, Maize, potato, and soybean) with first and co-authored 11 papers published in peer-reviewed journals such as Nature (co-first author), PNAS, Trends in Plant Science, Plant Cell, and etc.) 10 years of experience in cloning techniques and vector development for bacterial and plants Solid skills/expertise in molecular biology and plant transformation across multiple species Extensive technical knowledge of Next Generation Sequencing (NGS) applications (library analyze and preparation techniques, target enrichment, competitive NGS technologies) Expertise in development and execution of new protocol and pipelines in molecular biology, genetic, and biochemistry based research methods Excellent and strong analytical skills Extensive bioinformatics research experience Hands-on experience in developing bioinformatics algorism and pipelines for NGS data analysis 2 years of programming experience in Python, Unix, and R 4 years of experience in Linux, high performance computing environment, cluster computing and shell scripting 5 years of experience in operating different bioinformatics software in Linux/Unix OS, Sequencher program, as well as image processing software None-technical aspects Excellent communication skill and team player Quick at identifying questions and developing solutions Experience in hire, supervise, mentor, and train research associates and graduate students Lab management and administration experience. Responsibilities included ordering products, overseeing the repair of lab equipment, and many other administrative duties. These duties were performed while successfully executing research, publishing papers, and being a teaching assistant. Handling multi project Experience presenting at both international conferences and departmental meetings Experience in writing/publishing scientific manuscripts and patenting research result
DNA/RNA isolation (polyA RNA, lncRNA, small RNA etc), 5' and 3' RACE, molecular cloning and construct design, qPCR, northern blot, southern blot, small RNA blot, SSLP, AFLP, preparation of next generation-sequencing libraries (RNAseq, ChIPseq, RNA-IPseq), preparation of oligo microarray library
Protein expression, purification, and biochemistry: recombinant protein expression in bacteria, western blot, biotin-labed histone binding assay, Chromatin immunoprecipitation assay, RNA immunoprecipitation assay, RNA in vitro pull-down assay
Programming languages: Python, R, bash shell, awk
Bioinformatics and NGS analysis tools and database: Tuxedo suite tools, SAMtools, bedtools, BWA, DEseq2, EdgeR, BLAST, MACS2, ENCODE, ENSEMBL, IGV, SSH secure shell, Sequencher
Plant cultivation and breeding: propagation of Arabidopsis, potato, garlic, and soybean in greenhouse, field, and growth chamber condition, Tissue culture of Arabidopsis, potato, garlic, and soybean
Transgenic plant skills: cultivation and generation of transgenic Arabidopsis, potato, garlic, and soybean using
Cloning techniques: generated ~100 constructs and ~50 mutations using site-directed mutagenesis
Abiotic and biotic stress screening: Cereal rust pathogen handling, inoculation, storage, and scoring; hydroponic salt stress assays, salicylic acid treatment, cold stress assays, and pathogen resistance test
Physiological measurement: analysis of elements and metabolites such as starch and chlorophyll in plant tissues
01/2011 to 01/2016
Postdoctoral Research AssociateThe University of Texas at Austin － Austin, TX
Cell-type specific regulation during maize endosperm development Analyzed spatiotemporal specific transcriptome profiles from dissected maize endosperm using cryo-dissection method and RNA sequencing Identified transcriptional regulatory networks of spatiotemporal specific coding and long noncoding transcripts using a custom bioinformatics pipeline and validated the targets with qRT-PCR, qChIP-PCR, and RNA in situ hybridization Collaborated with scientists in The University of Florida and The University of Texas at Austin Studies resulted in first and co-authored publication (one manuscript under review) Project 2.
Epigenetic mechanism of floral transition by functional association of Polycomb repressive complex2 (PRC2) and long noncoding Uncovered how PRC2 complex is recognized their genomic target sites using RNAseq, ChIPseq, and RNA-IPseq Developed and implemented new experimental protocols to examine long and small noncoding RNA and their interaction with protein complex in vivo and in vitro Implemented bioinformatics algorithms and develop pipelines for RNA-IP (RNA immunoprecipitation sequencing, ChIPseq, and RNAseq analysis) Developed library construction of RNA-IPseq, ChIPseq, and RNAseq and data analysis Characterized the function of novel lncRNAs utilizing genetic screens, DNAseq, PCR genotyping, and molecular analysis Collaborated with scientists in Seoul National University in Korea and The University of Texas at Austin Supervised two research associates and one graduate student Studies resulted in 2 first-author publications (one manuscript in preparation.
01/2005 to 01/2010
Graduate Research AssistantThe University of Texas at Austin － Austin, TX
The molecular mechanism of heterosis in allotetraploids and hybrids Discovered a locus-specific and chromatin regulation (e.g.
H3K9 acetylation and H3K4di-methylation) of circadian clock genes in the allopolyploids leading to hybrid vigor using oligo microarray and targeted approaches.
Generated and characterized mutant lines in allopolyploids and epigenetic mutant lines Collaborated with scientist in China Agricultural University and computer scientists in The University of Texas at Austin Supervised three research associates and one graduate student Studies resulted in a patent application of which I am co-inventor (Application # 14147408) and co-first author publication in Nature Project 2.
Non-additive gene regulation upon allopolyploidization at post transcriptional level Initiated project and explored a role of posttranscriptional regulation in non-additive gene expression using oligo microarray Developed new experiment protocol to measure mRNA decay rate in allopolyploids Designed and spotted oligo gene microarray slides in house and constructed cDNA library Supervised and managed microarray printing machine Developed a strong genome wide analysis base Studies resulted in first author publication in PLosOne Project 3.
The role of duplicated genes after Whole genome duplication (WGD) Designed and spotted oligo gene microarray slides in house and constructed cDNA library Collaborated with graduate student and resulted in co-authored publication in PNAS Project 4.
Parent-of-origin effects on circadian rhythms and growth vigor in hybrids Initiated project and collaborated 3 graduate students and 2 postdocs Studies resulted in co-authored publication in Plant Cell.
01/2001 to 01/2003
Graduate Research AssistantSeoul National University － Seoul, KOREA
Characterized biological function of a Methyl Jasmonate-inducible gene MJI3 in disease-resistance using cDNA microarray, northern blot, pathogen resistance test, and transgenic approaches.
Improved method of garlic (Allium sativum L.) plant transformation by biolistic gene transformation (particle bombardment).
Generated basta resistant transgenic soybean using meristem transformation and laser transformation.
Undergraduate Research AssistantPohang University of Science and Technology － Pohang, KOREA
Developed a strong research base in Arabidopsis thaliana.
Characterized mutant lines using PCR genotyping and RT-PCR.
01/1998 to 01/2001
Undergraduate Research AssistantKangwon National University － Chuncheon, KOREA
Potato breeding and transformation analysis using tissue culture based method.
Production and Characterization of CMS (Cytoplasmic Male Sterility) Lines in Brassica Species.
Ph.D: Plant BiologyThe University of Texas － AustinU.S.A.Plant Biology
M.S: Applied Biology and ChemistrySeoul National UniversityKOREAApplied Biology and Chemistry
B.A.: Applied Plant ScienceKangwon National UniversityApplied Plant Science
Ph.DThe Chinese University of Hong Kong
School of Life SciencesStanley J Roux Jr, Ph.D. Professor, The University of Texas at Austin Molecular Biosciences, College of Natural Sciences Phone: 1 512 471 4238 firstname.lastname@example.org Byung Ho Kang
Invitation to Review for manuscript submitted to Environmental Epigenetic journal, 2015 Invitation to Review for manuscript submitted to Plant Cell Report journal, 2015 Poster presentation at the 2012 KSEA Central Texas Regional Conference, Austin, Texas, 2012 Poster presentation in the 9th International Plant Molecular Biology Congress, St.
Lousis, Missouri, 2009 Presenter in the Plant Biology Meeting, MCDB, The University of Texas at Austin, 2008.
Ni, Z.*, Kim, E.D.*, et. al. (2009) Nature 457, 327-331. *Equal contribution as the first author Ha, M., Kim, E.D., et. al. (2009) Proc. Natl. Acad. Sci. USA 106, 2295-2300 Kim, E.D., Chen,Z.J. (2011) PLoS One. 6(8), e24251a Kim, E.D., Sung, S. (2012) Trends in Plant Sci. 17, 16-21 Ng, DW., Miller, M., Yu, HH., Huang, TY., Kim, E.D., et. al. (2014) Plant Cell. 26(6):2430-2440 Xiong, Y., Mei, W., Kim, E.D., et. al. (2014) BMC Plant Biol. 14, 204 Kim, E.D., et. al. (2016) (submitted to Proc. Natl. Acad. Sci. USA) Kim, E.D. et. al. (2004) Agric. Chem. Biotechnol. 47, 129-132. Cheong, J.J., Jang, G.P., Jung, C., Kim, E.D., et. al. (2001) Annals of the Research Center for New Bio-Materials in Agriculture 11, 114-120. Lee, H.Y., Kim, E.D., et. al. (2001) Plant Molecular Genetics and Breeding, 12-25 Cheong, J.J., Jung, C., Kim, E.D., et. al. (2001) Annals of the Research Center for New Bio-Materials in Agriculture 10, 122-129. In Preparation Kim, E.D., Sung,S. Coordinated regulation of floral transition by protein and long noncoding RNA components Patent Application number 14/147,408. Molecular clock mechanism of hybrid vigor. Chen, Z.J., Kim, E.D., Zhongfu, N. (2014)
Volunteer, Food distribution to homeless people, 2005
*Korean-American Scientists & Engineers Association (KSEA) manger in Austin chapter, 2012-2016
*Volunteer staff and proctor for National Mathematics & Science Competition (NMSC), 2012-2015
*Volunteer reading books and converting books to e-text audio book at disability resource center, 1998
HONER AND AWARDS Research Travel Grant, Scientist and Engineers Early-Career Development, 2012 Outstanding Poster Award, Korean-American Scientists and Engineers Association, 2012 David Bruton Jr Graduate School Fellowship, The University of Texas at Austin, 2009 Pete Oliver Memorial Endowment for Genetic Research Award, The University of Texas at Austin, 2009 Research Travel Award, IPMB (International Plant Molecular Biology) meeting, 2009 MEPS fellowship, Texas A&M University, College Station, Texas, 2004-2005 The Brain Korea 21 Projects Scholarship, The Ministry of Education, KOREA, 2001-2003 Scholarship for excellent academic record present, Kangwon National University, KOREA, 1998-2001 SERVIS AND OUTREACH Volunteer, Food distribution to homeless people, 2005 Korean-American Scientists & Engineers Association (KSEA) manger in Austin chapter, 2012-2016 Volunteer staff and proctor for National Mathematics & Science Competition (NMSC), 2012-2015 Volunteer reading books and converting books to e-text audio book at disability resource center, 1998