Research Biologist with over 7 years in laboratory research. Accomplished in cell culture as well as in vivo models of brain disorders. Expertise in generating and applying new protocols and technologies. Recognized for adapting quickly to various scientific research learning environments. Diverse educational foundation incorporating several scientific disciplines (Neuroscience, Biochemistry, Zoology) providing a multifaceted approach to research initiatives.
MS Office proficiency
01/2015 to Current
Animal Care Specialist (part time 20hr/wk)URSINUS COLLEGE － COLLEGEVILLE, PA
Breed and propagate rodent and zebrafish colonies.
Train personnel in proper animal handling and procedures as per IACUC standards
Organize, label and restock supplies in the vivarium.
Collaborate with several faculty on cell culture and animal behavior research
Assist in minor procedures and monitor animal health
12/2013 to 06/2015
Research Associate (this grant funded position officially ended in Jan 2015) URSINUS COLLEGE － COLLEGEVILLE, PA
Conducted research in early neurodevelopment using zebrafish as a model organism.
Performed experiments to fulfill research objectives of the project, which studied binding partners and mechanisms of action of the Slitrk family of proteins known to play a role in functions like axon guidance, synapse development and neurogenesis.
Trained students on laboratory functions.
Maintained required laboratory safety protocols.
Ordered required supplies for the lab and maintained lab safety standards
Maintained detailed experimental records
01/2007 to 11/2008
Postdoctoral Research AssociateUNIVERSITY OF ROCHESTER － Rochester, NY
Conducted research in Neuron-glia interactions and Parkinson's disease.
Explored in-vivo models of Parkinson's disease (MPTP, paraquat), to understand the mechanism and extent of astrocyte involvement in regulating levels of neurotoxic cations and thus affecting neurodegeneration.
Designed and directed experiments using multiple wet lab molecular techniques to specifically study a transmembrane cation transporter (OCT-3) in the key hypotheses of the project
Trained personnel on laboratory functions.
Comprehensive knowledge of various animal models of parkinson's disease. Findings published in PNAS, co-first author on the paper
01/2000 to 08/2006
Research AssistantUNIVERSITY OF SOUTHERN CALIFORNIA － Los Angeles, CA
Conducted research in astrocyte reactivity and neurodegenerative disorders .
Study explored the non-apoptotic function of caspases in astrocytes.
Created a novel ex-vivo model of astrogliosis.
Research generated a novel finding of non-apoptotic caspase activation in reactive astrocyte.
PhD: NeuroscienceUNIVERSITY OF SOUTHERN CALIFORNIA － Los Angeles, CANeuroscience Dissertation topic: Non-apoptotic role of caspases in astrogliosis
Master of Science: BiochemistryMAHARAJA SAYAJIRAO UNIVERSITYIndiaBiochemistry Dissertation topic: Comparison of anti-oxidant systems in drug-na�ve versus drug-treated
Bachelor of Science: Zoology MAHARAJA SYAJIRAO UNIVERSITY － VadodaraINDIAMinor in Botany and Chemistry
Glial-neuronal interactions: Astrocytes modulate neurodegeneration via the extraneuronal monoamine transporter in the MPTP mouse model of Parkinson's disease. RV Aras, P Rappold, M. Hatwar, W. Peng, V. Jackson-Lewis, N. Ballatori, J. A. Javitch, M. Nedergaard, S. Przedborski , K. Tieu; SFN 2007
Non-apoptotic role for caspase activation in an ex-vivo model of astrogliosis. RV Aras, CJ Pike; SFN 2004
Characterization of caspases involved in astrogliosis. RV Aras, T. Vateresian, CJ Pike; SFN 2002
Role of caspase activation in astrogliosis. RV Aras, C. Tu, T. Shin, CJ Pike; SFN 2001
“The organic cation transporter-3 is a pivotal modulator of neurodegeneration in the nigrostriatal dopaminergic pathway.” Cui M, Aras R, Christian WV, Rappold PM, Hatwar M, Panza J, Jackson-Lewis V, Javitch JA, Ballatori N, Przedborski S, Tieu K. (Proc Natl Acad Sci U S A. 2009 May 12;106(19):8043-8. Epub 2009 Apr 29).
“Caspase activation contributes to astrogliosis.” Aras R, Barron AM and Pike CJ (Brain Research 2012 Mar 1)
“Using Swiss Webster mice to model Fetal Alcohol Spectrum Disorders (FASD): an analysis of multilevel time-to-event data through mixed-effects Cox proportional hazards models” Peter Chi; Radha Aras; Katie Martin; and Carlita Favero (Behavioral Brain Research, 2016 Jan 4. pii: S0166-4328(15)30349-1. doi: 10.1016/j.bbr.2015.12.040. [Epub ahead of print])
Cell culture: Expertise in primary astrocyte and neurons cultures from embryonic and adult mice and rats as well as variety of cell lines (Hek 293, Cos, breast cancer MCF). Also have experience with astrocyte-neuron co-cultures (contact & non-contact models)
Immuno-staining techniques: Expertise in different methods of immuno-staining perfused or frozen brain sections and cells in culture. Fluorescence or biotinylated tagged single and double labeling of brain tissue.
Histology: Expertise in gross and fine brain tissue dissection to harvest specific brain regions. Proficient in use of cryostat as well as vibrotome.
Biochemistry and molecular biology techniques: Expertise in western blotting, PCR, as well as DNA-RNA isolation from cells, and different tissues.
Animal husbandry: Proficient in rodent handling, breeding, genotyping. Extensive experience in injections, blood collections from tail veined harvesting of tissue.
CNS Disease models: extensive knowledge and experience working with the following CNS disease models in rodents MPTP model of parkinson's disease, Kainate excitotoxicity model used in epilepsy and schizophrenia research
Mouse behavior: Experience in testing for developmental milestones in mice pups (eg surface righting, negative geotaxis, etc)
Specific techniques for zebrafish model: Microinjection of embryos, immunohistochemistry