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Advanced Biochemistry Research with Dr. Melanie Berkmen Resume Example

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ADVANCED BIOCHEMISTRY RESEARCH WITH DR. MELANIE BERKMEN
Summary
Undergraduate student at Suffolk University majoring in Biochemistry. Interested in obtaining a position in the biological sciences before applying to a PhD program. Offers knowledge of laboratory equipment, safety practices and biohazard disposal and is accomplished in several biochemical techniques such as Western Blotting, Pipetting, Bacterial Cell cultures, transformations, Plasmid Isolation and Polymerase Chain Reaction. Interested in learning new laboratory techniques and doing research in molecular biology or genetics and drug development.
Education
Bachelor of Arts:Biochemistry2017Suffolk University , Boston , MA
GPA: 3.81
CAS High Honor's Dean List: Fall 2013-Fall 2015
CAS Honor's Dean List: Spring 2016-Fall 2016
Skills
Research Skills: Bacterial Cell culture, Transformations, Betagalactosidase Assays, Plasmid and Genome DNA isolation, Western Blotting, Flow cytometry, Buffer preparation, Autoclave
Other skills: Public speaker, Team player, Microsoft Excel, Word, Powerpoint, Fluent in English and French  
Experience
Suffolk Universiy Department of Chemistry and BiochemistryBoston, MAAdvanced Biochemistry Research with Dr. Melanie Berkmen01/2017 to 05/2017
  • Read primary literature related to the highly conserved Kreb's cycle enzyme fumarase, fumarase deficiency, leiomyomatosis and renal cell cancer related to mutations in fumarase
  • Utilized site-directed mutagenesis to make mutation Met195Thr in human recombinant His6-tagged fumarase
  • Purified and characterized protein, discovering that fumarase (Met195Thr) has reduced activity in vitro compared to the wild-type enzyme
Suffolk University Department of BiologyBoston, MASenior Researcher09/2016 to 05/2017
  • Carried out a forward genetic screen to find novel regulators of ​rpoS​ in ​E. coli
  • Isolated a plasmid containing an intergenic DNA transcript that may regulate the rpos gene in E. coli
  • Determined the mechanism by which the regulator affected rpoS by using several different rpoS'-'lacZ fusions
  • Performed many Betagalactosidase assays on different E. coli strains
  • Successfully cloned an E. coli strain lacking the 5'UTR of ChiP 
Bacteriology and Virology Laboratory, LeDantec Hospital Dakar, SenegalIntern06/2016 to 09/2016
  • Measured CD4 T cell levels in blood samples from hundreds of HIV positive patients using Flow Cytometry
  • Performed dozens of HIV blood tests on patients
  • Performed Cell Separation with blood samples to isolate peripheral mononuclear blood cells from erythrocytes
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Resume Overview

Companies Worked For:

  • Suffolk Universiy Department of Chemistry and Biochemistry
  • Suffolk University Department of Biology
  • Bacteriology and Virology Laboratory, LeDantec Hospital

School Attended

  • Suffolk University

Job Titles Held:

  • Advanced Biochemistry Research with Dr. Melanie Berkmen
  • Senior Researcher
  • Intern

Degrees

  • Bachelor of Arts : Biochemistry 2017

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