Molecular and cellular virologist and biochemist with over 14 years of experience in a research laboratory setting
Developed several antiviral compounds against 3 HIV-1 targets that resulted in >25 peer reviewed publications, several invited talks, and multiple funding renewals
Coordinated and managed high-level independent extramural and intramural collaborative research projects in BSL-2 lab setting
Strong experience working in areas of molecular biology, virology, vector biology, and cell biology
Used in vitro techniques and conducted cellular based assays to identify the specificity, antiviral activity, and cytotoxicity of candidate HIV-1 drugs at a superior proficiency and in a BSL-2 setting
Extensive knowledge in mammalian cell biology, tissue culture, virus production, viral vector design, virus-host interactions, viral pathogenesis and propagation, and titration
Advanced skills in PCR and molecular cloning and biology techniques including DNA/RNA extraction, purification, mutagenesis, quantification, and broad range of virological techniques
Extensive experience in viral gene/protein expression analyses, ECL and PCR analytical equipment and knowledge in next generation sequencing
Ability and willing to work in changing environment, different biosafety levels, BPRP, CDC select agent program approval and any other trainings/requirements
Organized data records and lab notebooks and complied with specified safety and security guidelines and proper standard operating procedures
Published manuscripts in peer-reviewed journals, wrote experimental methods and provided data for EIRs and patents
Demonstrated excellent communication and organization skills
Provided support and prepared technical reports and presented at nationally recognized scientific meetings
Managed junior scientists, technicians, students, and support personnel and acted as liaison between teams and senior investigators
Maintaining cell culture lines and assay analysis, antiviral and cell cytotoxicity drug screenings, subcellular protein localization
Biochemical methods including gel electrophoresis, Western blots, ELISAs, protein pulldown assays, co-Immunoprecipitations, protein expression and isolation, circular dichroism spectroscopy, electron microscopy, chemical cross-linking, dynamic light scattering, analytical ultracentrifugation, gel filtration chromatography
Molecular biology methods such as cloning, PCR, site-directed mutagenesis, Ligation-mediated PCR (LM-PCR), large-scale bacterial preps, plasmid characterization, oligo radiolabeling, nucleic acid extraction using robotics
Virology techniques such as virus production and titer measurement
Microsoft office, Adobe Photoshop
Research Biologist10/2017 to Current National Institutes of Health, National Cancer Institute- HIV DRP – Frederick, MD
Identified and developed INSTIs to evaluate in preclinical studies.
Evaluated FDA-approved INSTIs against panels of INSTI-resistant mutants.
Conducted in-depth analysis in silico on FDA-approved INSTI.
Research Fellow10/2013 to 01/2017 National Institutes of Health, National Cancer Institute- HIV DRP – Frederick, MD
Determined and directed the development of several HIV-1 Integrase Strand Transfer Inhibitors (INSTIs) for further evaluation in rodents to identify pharmacokinetic and pharmacodynamics properties.
Conducted in-depth analysis on 2 clinical non-nucleoside Reverse Transcriptase Inhibitors (NNRTIs) to combat HIV-1.
Streamlined in vitro cellular assays to efficiently screen compounds for antiviral activity and cellular cytotoxicity by 50%.
Increased the efficiency of HIV-1 resistant mutant design and virus production by 50%.
Generated in vitro antiviral data for >15 NNRTIs against >25 NNRTI resistant HIV-1 mutants.
Constructed 4 new structural models and simulations of HIV-1 Integrase.
Evaluated >10 lead compounds against novel HIV-1 drug target by in vitro studies.
Postdoctoral Research Fellow........................10/2008 to 01/2013 National Institutes of Health, National Cancer Institute- HIV DRP......... – Frederick, MD
Evaluated and Identified >100 HIV-1 INSTIs.
Measured cellular cytotoxicity and antiviral activity of HIV-1 INSTIs against WT and resistant mutant HIV-1 viruses.
Generated > 75 mutant HIV-1 viruses to determine viral fitness and susceptibilities to HIV-1 INSTIs.
Designed and modeled HIV-1 integrase strand transfer inhibitors.
Analyzed HIV-1 viral DNA integration sites within the host genome.
Graduate Student..............................08/2004 to 01/2008 University of Kentucky – Lexington, KY
Characterized the actin binding site within Talin1.
Used gel filtration chromatography, chemical cross-linking, analytical ultracentrifugation, and dynamic light scattering to determine that protein oligomerization is necessary for Talin1 function.
Purified recombinant mutant proteins for in vitro studies.
Determined protein localization in vivo using immunofluorescence microscopy and GFP-tagged proteins.
Analyzed actin filament organization with Talin1 mutants with electron microscopy.
PhDUniversity of Kentucky-
KYMolecular and Cellular Biochemistry,
Concentration in Molecular Biology, Protein Biochemistry, Structure, and Biophysics
Thesis: Biochemical Characterization of the Conserved C-terminal I/LWEQ Module of Talin1
Bachelor of Arts: Chemistry and Exercise ScienceMcDaniel College-