Dynamic and versatile Research Associate with extensive detailed research and experimental experience. Holds a broad spectrum of technical, experimental and analytical skills that would benefit any organization. Expertise in many areas of research that utilize in vivo, ex-vivo and in-vitro skills and abilities.
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Protein techniques: Protein isolation and quantification, Western blotting, Immunoprecipitation.
DNA/RNA techniques: Genotyping of mice by utilizing gel electrophoresis, DNA extraction and PCR techniques. Isolation and quantification of DNA and RNA and preparing plasmid constructs and plasmid isolation.
Cell biology techniques: Isolation of HUVEC, Aortic and lung EC from mice, Tissue/cell culture, cell count (hemocytometer) and cell viability assays, Maintaining immortalized cell lines, Gene editing (preferred), Immunofluorescence staining, and DNA transfection.
Performed general duties, ordering of supplies, making solutions, buffers, and culture media as directed by the supervisor.
Assembled and operated laboratory apparatus on daily basis while ensuring lab safety and conducted equipment maintenance on a weekly basis.
Maintained lab notebooks and prepared SOPs in accordance with GLPs.
Planned, modified, and executed research techniques, procedures and tests.
Protein techniques: Bacterial transformation, Induction and Expression of proteins with Separation and Purification using affinity chromatography.
Cell biology techniques: Cryopreserving cells, Maintaining and Passaging in-vivo and ex-vivo cultured cells, Cycle count, Hematopoietic Stem Cell isolation, Crystallization, IMAC, SDS-PAGE techniques when working with cultured cells in various assays.
MS Office, PeopleSoft, JMP Software
Proficient with Microsoft Windows XP/7/8/10, Mac OS
Sivareddy Kotla, 1 Hang Thi Vu, 1 Kyung Ae Ko, 1 Yin Wang, 1 Masaki Imanishi, 1 Kyung-Sun Heo, 1 Yuka Fujii, 1 Tamlyn N. Thomas, 1 Young Jin Gi, 1 Hira Mazhar, 1 Jesus Paez-Mayorga, 2,3 Ji-Hyun Shin, 1 Yunting Tao, 2 Carolyn J. Giancursio, 2 Jan L.M. Medi. Endothelial senescence is induced by phosphorylation and nuclear export of telomeric repeat binding factor 2–interacting protein. 4 April 2019.
Drug Metabolism, Cell culture, Pharmacokinetics, Biostatistics, Biochemistry, Physiology, Immunology, Cell and Molecular Biology, Organic Chemistry.
Protein techniques: Protein isolation and quantification, Western blotting, Immunoprecipitation.
DNA/RNA techniques: Genotyping of mice by utilizing gel electrophoresis, DNA extraction and PCR techniques. Isolation and quantification of DNA and RNA and preparing plasmid constructs and plasmid isolation.
Cell biology techniques: Isolation of HUVEC, Aortic and lung EC from mice, Tissue/cell culture, cell count (hemocytometer) and cell viability assays, Maintaining immortalized cell lines, Gene editing (preferred), Immunofluorescence staining, and DNA transfection.
Performed general duties, ordering of supplies, making solutions, buffers, and culture media as directed by the supervisor.
Assembled and operated laboratory apparatus on daily basis while ensuring lab safety and conducted equipment maintenance on a weekly basis.
Maintained lab notebooks and prepared SOPs in accordance with GLPs.
Planned, modified, and executed research techniques, procedures and tests.
Protein techniques: Bacterial transformation, Induction and Expression of proteins with Separation and Purification using affinity chromatography.
Cell biology techniques: Cryopreserving cells, Maintaining and Passaging in-vivo and ex-vivo cultured cells, Cycle count, Hematopoietic Stem Cell isolation, Crystallization, IMAC, SDS-PAGE techniques when working with cultured cells in various assays.
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