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Research Assistant II Resume Example

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RESEARCH ASSISTANT II
Professional Summary

Dynamic and versatile Research Associate with extensive detailed research and experimental experience. Holds a broad spectrum of technical, experimental and analytical skills that would benefit any organization. Expertise in many areas of research that utilize in vivo, ex-vivo and in-vitro skills and abilities.

Skills
  • Histology, Tissue/cell isolation, Cell Culture,Immunoprecipitation,
  • PCR & RT-PCR, DNA/ Agarose Gel Electrophoresis, SDS-PAGE
  • GC-MS/HPLC, Solid phase extractions/ Lipid extractions.
  • Protein Isolation and Purification & Western Blotting
  • Buffer preparations, DNA Amplification, Quantification & Analysis
  • Plasmid Extraction & Cloning, Affinity & Thin Layer Chromatography
  • Fluorescence & IR Spectroscopy
  • DNA Amplification, Quantification & Analysis.
  • Serial Dilutions, Sterile Technique, Inventory and supply management
  • Research design & Literature reviews, Data collection and analysis
  • Lab safety & Equipment management
  • Project management, Experimental design
Work History
Research Assistant II, 03/2018 to 06/2019
UT MD Anderson Cancer Center – Houston, TX
  • (15 months) project: Histology work which included harvesting mouse tissue, taking gross imaging and compiling data for analysis
  • Conducted experiments utilizing:

Protein techniques: Protein isolation and quantification, Western blotting, Immunoprecipitation.

DNA/RNA techniques: Genotyping of mice by utilizing gel electrophoresis, DNA extraction and PCR techniques. Isolation and quantification of DNA and RNA and preparing plasmid constructs and plasmid isolation.

Cell biology techniques: Isolation of HUVEC, Aortic and lung EC from mice, Tissue/cell culture, cell count (hemocytometer) and cell viability assays, Maintaining immortalized cell lines, Gene editing (preferred), Immunofluorescence staining, and DNA transfection.

  • Additional experience:

Performed general duties, ordering of supplies, making solutions, buffers, and culture media as directed by the supervisor.

Assembled and operated laboratory apparatus on daily basis while ensuring lab safety and conducted equipment maintenance on a weekly basis.

Maintained lab notebooks and prepared SOPs in accordance with GLPs.

Planned, modified, and executed research techniques, procedures and tests.

Graduate Research Assistant, 05/2017 to 12/2017
University of Massachusetts Lowell, Pharmaceutical analysis Lab – Lowell, MA
  • (7 months) Project: Monitored and quantified oxidative stress markers in body fluids of patients working with photocopiers.
  • Utilized IR spectrometry to analyze the concentration of samples; techniques utilized included UV-Vis/IR spectrophotometry & HPLC.
Graduate Research Assistant, 09/2016 to 12/2017
University Of Massachusetts Lowell – North Chelmsford, MA
  • (17 months) Project: Utilized GC-MS to Investigate and quantify lipids in livers and plasma of mice fed diet containing oxidized linoleic acid to confirm dietary consumption of linoleic acid in lowering triglyceride levels; techniques utilized included extraction and dilution.
  • Conducted experiments utilizing:

Protein techniques: Bacterial transformation, Induction and Expression of proteins with Separation and Purification using affinity chromatography.

Cell biology techniques: Cryopreserving cells, Maintaining and Passaging in-vivo and ex-vivo cultured cells, Cycle count, Hematopoietic Stem Cell isolation, Crystallization, IMAC, SDS-PAGE techniques when working with cultured cells in various assays.

Undergraduate Research Assistant, 01/2016 to 05/2016
University of Massachusetts Lowell – Lowell, MA
  • Interpreted research findings and summarized data into reports.
  • Evaluated collected data points from research experiments regarding fleshy wild fruits that are grown in tropics to produce summary reports for biological research.
Education
Masters: Science, Pharmaceutical Sciences, 2017
University of Massachusetts - Lowell - Lowell, MA
  • Summa cum laude graduate
  • 3.85 GPA
Bachelors of Science: Biology, 2016
University of Massachusetts - Lowell - Lowell, MA
Custom
  • Proficient in English, Urdu & Hindi
  • Strong Work Ethic, Good Communication Skills, Problem Solving Skills, Team Player, Flexible and Adaptable to Change, Perform well Under Pressure, Excellent advocacy skills, Quality Driven Productivity
Certifications
  • Outstanding Graduate Student Award in Pharmaceutical Sciences major: for exemplary performance in clinical setting.
  • Licensed Pharmacy Technician
Custom

MS Office, PeopleSoft, JMP Software

Proficient with Microsoft Windows XP/7/8/10, Mac OS

Custom
  • CVS Pharmacy- Pharmacy Technician /Lowell, MA / 09/2016-11/2017
  • Macys-Sales Associate/ Nashua-NH/ 05/2013-09/2013
  • UMass- Clerical Support Associate/ Lowell, MA/ 09/2012-05/2013
  • UMass- Teaching Intern/ Lowell, MA/ 09/2012-05/2013
Custom

Sivareddy Kotla, 1 Hang Thi Vu, 1 Kyung Ae Ko, 1 Yin Wang, 1 Masaki Imanishi, 1 Kyung-Sun Heo, 1 Yuka Fujii, 1 Tamlyn N. Thomas, 1 Young Jin Gi, 1 Hira Mazhar, 1 Jesus Paez-Mayorga, 2,3 Ji-Hyun Shin, 1 Yunting Tao, 2 Carolyn J. Giancursio, 2 Jan L.M. Medi. Endothelial senescence is induced by phosphorylation and nuclear export of telomeric repeat binding factor 2–interacting protein. 4 April 2019. .

Custom

Drug Metabolism, Cell culture, Pharmacokinetics, Biostatistics, Biochemistry, Physiology, Immunology, Cell and Molecular Biology, Organic Chemistry.

Skills
  • Histology, Tissue/cell isolation, Cell Culture,Immunoprecipitation,
  • PCR & RT-PCR, DNA/ Agarose Gel Electrophoresis, SDS-PAGE
  • GC-MS/HPLC, Solid phase extractions/ Lipid extractions.
  • Protein Isolation and Purification & Western Blotting
  • Buffer preparations, DNA Amplification, Quantification & Analysis
  • Plasmid Extraction & Cloning, Affinity & Thin Layer Chromatography
  • Fluorescence & IR Spectroscopy
  • DNA Amplification, Quantification & Analysis.
  • Serial Dilutions, Sterile Technique, Inventory and supply management
  • Research design & Literature reviews, Data collection and analysis
  • Lab safety & Equipment management
  • Project management, Experimental design
Work History
Research Assistant II, 03/2018 to 06/2019
UT MD Anderson Cancer Center – Houston, TX
  • (15 months) project: Histology work which included harvesting mouse tissue, taking gross imaging and compiling data for analysis
  • Conducted experiments utilizing:

Protein techniques: Protein isolation and quantification, Western blotting, Immunoprecipitation.

DNA/RNA techniques: Genotyping of mice by utilizing gel electrophoresis, DNA extraction and PCR techniques. Isolation and quantification of DNA and RNA and preparing plasmid constructs and plasmid isolation.

Cell biology techniques: Isolation of HUVEC, Aortic and lung EC from mice, Tissue/cell culture, cell count (hemocytometer) and cell viability assays, Maintaining immortalized cell lines, Gene editing (preferred), Immunofluorescence staining, and DNA transfection.

  • Additional experience:

Performed general duties, ordering of supplies, making solutions, buffers, and culture media as directed by the supervisor.

Assembled and operated laboratory apparatus on daily basis while ensuring lab safety and conducted equipment maintenance on a weekly basis.

Maintained lab notebooks and prepared SOPs in accordance with GLPs.

Planned, modified, and executed research techniques, procedures and tests.

Graduate Research Assistant, 05/2017 to 12/2017
University of Massachusetts Lowell, Pharmaceutical analysis Lab – Lowell, MA
  • (7 months) Project: Monitored and quantified oxidative stress markers in body fluids of patients working with photocopiers.
  • Utilized IR spectrometry to analyze the concentration of samples; techniques utilized included UV-Vis/IR spectrophotometry & HPLC.
Graduate Research Assistant, 09/2016 to 12/2017
University Of Massachusetts Lowell – North Chelmsford, MA
  • (17 months) Project: Utilized GC-MS to Investigate and quantify lipids in livers and plasma of mice fed diet containing oxidized linoleic acid to confirm dietary consumption of linoleic acid in lowering triglyceride levels; techniques utilized included extraction and dilution.
  • Conducted experiments utilizing:

Protein techniques: Bacterial transformation, Induction and Expression of proteins with Separation and Purification using affinity chromatography.

Cell biology techniques: Cryopreserving cells, Maintaining and Passaging in-vivo and ex-vivo cultured cells, Cycle count, Hematopoietic Stem Cell isolation, Crystallization, IMAC, SDS-PAGE techniques when working with cultured cells in various assays.

Undergraduate Research Assistant, 01/2016 to 05/2016
University of Massachusetts Lowell – Lowell, MA
  • Interpreted research findings and summarized data into reports.
  • Evaluated collected data points from research experiments regarding fleshy wild fruits that are grown in tropics to produce summary reports for biological research.
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Resume Overview

Companies Worked For:

  • UT MD Anderson Cancer Center
  • University of Massachusetts Lowell, Pharmaceutical analysis Lab
  • University Of Massachusetts Lowell
  • University of Massachusetts Lowell

School Attended

  • University of Massachusetts - Lowell

Job Titles Held:

  • Research Assistant II
  • Graduate Research Assistant
  • Undergraduate Research Assistant

Degrees

  • Masters : Science, Pharmaceutical Sciences , 2017
    Bachelors of Science : Biology , 2016

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