Research Assistant II Resume Example
Dynamic and versatile Research Associate with extensive detailed research and experimental experience. Holds a broad spectrum of technical, experimental and analytical skills that would benefit any organization. Expertise in many areas of research that utilize in vivo, ex-vivo and in-vitro skills and abilities.
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Harvard University – Somerville , MA
- (15 months) project: Histology work which included harvesting mouse tissue, taking gross imaging and compiling data for analysis
- Conducted experiments utilizing:
Protein techniques: Protein isolation and quantification, Western blotting, Immunoprecipitation.
DNA/RNA techniques: Genotyping of mice by utilizing gel electrophoresis, DNA extraction and PCR techniques. Isolation and quantification of DNA and RNA and preparing plasmid constructs and plasmid isolation.
Cell biology techniques: Isolation of HUVEC, Aortic and lung EC from mice, Tissue/cell culture, cell count (hemocytometer) and cell viability assays, Maintaining immortalized cell lines, Gene editing (preferred), Immunofluorescence staining, and DNA transfection.
- Additional experience:
Performed general duties, ordering of supplies, making solutions, buffers, and culture media as directed by the supervisor.
Assembled and operated laboratory apparatus on daily basis while ensuring lab safety and conducted equipment maintenance on a weekly basis.
Maintained lab notebooks and prepared SOPs in accordance with GLPs.
Planned, modified, and executed research techniques, procedures and tests.
Association Of American Medical Colleges – Washington , DC
- (7 months) Project: Monitored and quantified oxidative stress markers in body fluids of patients working with photocopiers.
- Utilized IR spectrometry to analyze the concentration of samples; techniques utilized included UV-Vis/IR spectrophotometry & HPLC.
Association Of American Medical Colleges – Remote - Washington Dc / Baltimore Area , DC
- (17 months) Project: Utilized GC-MS to Investigate and quantify lipids in livers and plasma of mice fed diet containing oxidized linoleic acid to confirm dietary consumption of linoleic acid in lowering triglyceride levels; techniques utilized included extraction and dilution.
- Conducted experiments utilizing:
Protein techniques: Bacterial transformation, Induction and Expression of proteins with Separation and Purification using affinity chromatography.
Cell biology techniques: Cryopreserving cells, Maintaining and Passaging in-vivo and ex-vivo cultured cells, Cycle count, Hematopoietic Stem Cell isolation, Crystallization, IMAC, SDS-PAGE techniques when working with cultured cells in various assays.
University Of South Carolina – Salkehatchie , SC
- Interpreted research findings and summarized data into reports.
- Evaluated collected data points from research experiments regarding fleshy wild fruits that are grown in tropics to produce summary reports for biological research.
University of Massachusetts - Lowell - City, State
- Summa cum laude graduate
- 3.85 GPA
University of Massachusetts - Lowell - City, State
- Proficient in English, Urdu & Hindi
- Strong Work Ethic, Good Communication Skills, Problem Solving Skills, Team Player, Flexible and Adaptable to Change, Perform well Under Pressure, Excellent advocacy skills, Quality Driven Productivity
- Outstanding Graduate Student Award in Pharmaceutical Sciences major: for exemplary performance in clinical setting.
- Licensed Pharmacy Technician
MS Office, PeopleSoft, JMP Software
Proficient with Microsoft Windows XP/7/8/10, Mac OS
- CVS Pharmacy- Pharmacy Technician /Lowell, MA / 09/2016-11/2017
- Macys-Sales Associate/ Nashua-NH/ 05/2013-09/2013
- UMass- Clerical Support Associate/ Lowell, MA/ 09/2012-05/2013
- UMass- Teaching Intern/ Lowell, MA/ 09/2012-05/2013
Sivareddy Kotla, 1 Hang Thi Vu, 1 Kyung Ae Ko, 1 Yin Wang, 1 Masaki Imanishi, 1 Kyung-Sun Heo, 1 Yuka Fujii, 1 Tamlyn N. Thomas, 1 Young Jin Gi, 1 Hira Mazhar, 1 Jesus Paez-Mayorga, 2,3 Ji-Hyun Shin, 1 Yunting Tao, 2 Carolyn J. Giancursio, 2 Jan L.M. Medi. Endothelial senescence is induced by phosphorylation and nuclear export of telomeric repeat binding factor 2–interacting protein. 4 April 2019.
Drug Metabolism, Cell culture, Pharmacokinetics, Biostatistics, Biochemistry, Physiology, Immunology, Cell and Molecular Biology, Organic Chemistry.
- Histology, Tissue/cell isolation, Cell Culture,Immunoprecipitation,
- PCR & RT-PCR, DNA/ Agarose Gel Electrophoresis, SDS-PAGE
- GC-MS/HPLC, Solid phase extractions/ Lipid extractions.
- Protein Isolation and Purification & Western Blotting
- Buffer preparations, DNA Amplification, Quantification & Analysis
- Plasmid Extraction & Cloning, Affinity & Thin Layer Chromatography
- Fluorescence & IR Spectroscopy
- DNA Amplification, Quantification & Analysis.
- Serial Dilutions, Sterile Technique, Inventory and supply management
- Research design & Literature reviews, Data collection and analysis
- Lab safety & Equipment management
- Project management, Experimental design
Company Name – City, State
- (15 months) project: Histology work which included harvesting mouse tissue, taking gross imaging and compiling data for analysis
- Conducted experiments utilizing:
Protein techniques: Protein isolation and quantification, Western blotting, Immunoprecipitation.
DNA/RNA techniques: Genotyping of mice by utilizing gel electrophoresis, DNA extraction and PCR techniques. Isolation and quantification of DNA and RNA and preparing plasmid constructs and plasmid isolation.
Cell biology techniques: Isolation of HUVEC, Aortic and lung EC from mice, Tissue/cell culture, cell count (hemocytometer) and cell viability assays, Maintaining immortalized cell lines, Gene editing (preferred), Immunofluorescence staining, and DNA transfection.
- Additional experience:
Performed general duties, ordering of supplies, making solutions, buffers, and culture media as directed by the supervisor.
Assembled and operated laboratory apparatus on daily basis while ensuring lab safety and conducted equipment maintenance on a weekly basis.
Maintained lab notebooks and prepared SOPs in accordance with GLPs.
Planned, modified, and executed research techniques, procedures and tests.
Company Name – City, State
- (7 months) Project: Monitored and quantified oxidative stress markers in body fluids of patients working with photocopiers.
- Utilized IR spectrometry to analyze the concentration of samples; techniques utilized included UV-Vis/IR spectrophotometry & HPLC.
Company Name – City, State
- (17 months) Project: Utilized GC-MS to Investigate and quantify lipids in livers and plasma of mice fed diet containing oxidized linoleic acid to confirm dietary consumption of linoleic acid in lowering triglyceride levels; techniques utilized included extraction and dilution.
- Conducted experiments utilizing:
Protein techniques: Bacterial transformation, Induction and Expression of proteins with Separation and Purification using affinity chromatography.
Cell biology techniques: Cryopreserving cells, Maintaining and Passaging in-vivo and ex-vivo cultured cells, Cycle count, Hematopoietic Stem Cell isolation, Crystallization, IMAC, SDS-PAGE techniques when working with cultured cells in various assays.
Company Name – City, State
- Interpreted research findings and summarized data into reports.
- Evaluated collected data points from research experiments regarding fleshy wild fruits that are grown in tropics to produce summary reports for biological research.
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Resume Overview
School Attended
- University of Massachusetts - Lowell
Job Titles Held:
- Research Assistant II
- Graduate Research Assistant
- Undergraduate Research Assistant
Degrees
- Masters : Science, Pharmaceutical Sciences , 2017
Bachelors of Science : Biology , 2016
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