Highly motivated, recent Plant Science graduate (B.S. 2014) with over 7 years of hands-on field and laboratory experience including avian and mammalian cell culture, microbial culture, antibody production/purification, antibody-antigen capture ELISA, SDS-PAGE and Western Blot, immunoassay development/optimization, and various DNA/RNA sequencing techniques.
3.48 GPA, Elisha M. Rahn Memorial Scholarship recipient, Consistent member of Dean's List
Responsible for routine reagent production and testing for manufacture of IVD tests according to ISO 13485/cGMP regulations, as well as creation and execution of validation protocols/reports for product transfer and stability/FDA clinical studies, examination and determination of root cause analysis for non-conformities, execution of CAPA (corrective and preventative actions), identification and documentation of deviations, as well as for the creation and revision of various SOP's. Routinely performed IQ, OQ, PQ, and PM of various new and old laboratory equipment, including but not limited to: plate washers, plate readers, vortexes, centrifuges, incubators, pipettes, etc. Responsible for general laboratory maintenance and functionality, as well as conducted interview and training processes of new employees.
Responsible for biomarker identification and discovery related to sepsis, meningitis, osteoarthritis, rheumatoid arthritis, ALTR (adverse local tissue reaction) and periprosthetic joint infection, as well as native joint infection in human samples. Responsible for development and optimization of plate-based ELISA's (antibody-antigen capture, enzymatic, fluorescence) as well as development, optimization, and manufacture of lateral flow immunoassays targeting biomarkers of bacterial infections. Developed various immunogens, as well as assisted in the purification of various monoclonal and polyclonal antibodies by column chromatography. Responsible for/participated in screening of various clinical samples in relation to biomarkers, as well as analysis of data using Prism, SoftMax Pro and Microsoft Excel. Managed operation of microbiological laboratory, including, but not limited to: isolation/culture of various microbial pathogens using multiple techniques (i.e. plate streaking, broth culture, etc.), maintaining sterility, and creation of isolate stocks. Frequently utilized/optimized SDS-PAGE and Western blot immunodetection for identification/determination of small molecules and their reactivity to various antibodies. Responsible for general laboratory maintenance (i.e. maintenance of instruments/machines, liquid nitrogen tanks, biomedical waste, cleanliness, etc.). Assisted in interview, as well as hiring and training processes of new employees. Produced and presented information in R&D meetings related to current and previous studies performed. Responsible for the creation and maintenance of the largest synovial fluid sample inventory, as well as material inventories. Lead validation studies of LDT's for determination of bacterial infection of periprosthetic and native joints.
Responsible for management/maintenance of rose plots in viral (i.e. Rose rosette virus) pathogenicity/mobility field trials. Assisted in experimental field layout, set-up, and breakdown, including implementation and removal of watering systems. Participated in planting and removal of both lima beans and soy beans involved in fungal (i.e. Phytopthora spp.) pathogenicity trials.
Responsible for routine Avian Influenza (antibody-antigen capture lateral flow immunoassays) monitoring, routine serological analysis by antibody ELISA, data entry, and general laboratory maintenance. Responsible for in-ovo inoculation, incubation, and necropsy of avian embryos in various pathogenicity/vaccine development trials. Participated in avian field trials as well as necropsy, DNA/RNA sequencing of various avian viral pathogens, and assisted in tissue processing and isolation of viral/bacterial pathogens. Responsible for farm care and maintenance of various avian species including but not limited to: cleaning of facilities, removal of waste, collection of eggs, environmental control, feeding, watering, etc. during various pathogenicity and vaccine development trials.
Research and Development
Co-author of Presentation Abstracts
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