Graduate Student Research Assistant resume example with 9+ years of experience

Jessica Claire
, , 609 Johnson Ave., 49204, Tulsa, OK 100 Montgomery St. 10th Floor
Home: (555) 432-1000 - Cell: - - -

Motivated researcher with multidisciplinary formation in Microbiology, Molecular Biology and Nanotechnology


· Methodologies for the design of integrated diagnostic protocols in quarantine laboratories: Specificity, sensitivity, post-test likelihood ratios, Bayesian analyses, cost-benefit of detection protocols.

· Serology: Bacterial antigen preparation. Intramuscular, parenteral and subcutaneous rabbit immunization. Serum titration by double immunodiffusion. Protein A purification of IgG. ELISA optimization.

· Primers and probes design and optimization of conventional, multiplex and qPCR protocols.

· Nucleic acid extraction and manipulation for routine (PCR, qPCR, q-rtPCR) and next generation analyses (16S-ITS based metagenomics; transcriptomic analyses by RNAseq).

· Metagenomics analysis (16S for Bacteria and Archaea and 18S for Eukarya) and RNAseq-based transcriptomics analysis.

· Microscopy skills: Bright-field, time-lapse, contrast phase, transmission electron microscopy, inverted fluorescence and confocal laser scanning microscopy. Staining and targeting of specific organisms, structures and molecules with fluorescent dyes and antibodies. Experience with NIS-Elements and Image J (COMSTAT plugin) software’s for automatic cell counting, object movement tracking, and 3-D objects image analysis (area, height, and volume quantifications from z-stacks).

· Cellular growth dynamics and cell viability assessment: CFU/ml, cell counts by microscopy, turbidity-based growth curves, alamarBlue® cell proliferation kit, PMA-qPCR, LIVE/DEAD cellular viability kits.

· Evaluation of antimicrobials agents by clinical guidelines and other methods: minimum inhibitory concentration, minimum bactericidal concentration, time to kill assays, biofilm inhibition, killing and disruption activities.

· Study of toxicity mechanisms of antimicrobials: Intra and extracellular reactive oxygen species quantification, lipid peroxidation, cell content release, cell membrane integrity assessment, metal solubilization.

· Assessment of intra and extracellular chemical element composition by ICP-OES.

· Microfabrication: Microfluidic design with AutoDesk software, wafer manufacture by soft photolithography and 3D printing, manual microchip assembly (glass/PDMS for microscopy and PDMS/PDMS for other assays) and microfluidic control with Harvard apparatus and similar.

01/2016 to Current
Graduate Student Research Assistant Auburn University City, STATE,

Perform research in Microbiology, Molecular Biology, Analytical Chemistry, Next Generation Sequencing, Nanotechnology, and Microfluidics. Collaboration with research in the fields of Plant Pathology, Microbiology, Pharmacy and Aquiculture.

09/2008 to 10/2012
Specialist in Microbiology. National Center For Agrarian And Plant Health City, STATE,

Design and optimization of cultural, serological and molecular-based techniques for pathogenic bacteria identification.

Establishing methodologies for the design of integrated diagnostic protocols in quarantine laboratories.

BLS2 laboratory accreditation according to national biosafety guidelines.

07/2009 to 01/2012
Reserach Fellow Valencian Institute For Agrarian Research City, STATE,

Training in advanced methodologies to detect, identify and classify plant pathogenic bacteria

Education and Training
Expected in 07/2008
Bachelor of Science: Microbiology
Univeristy Of Havana - Cuba,
Expected in 05/2018
Master of Science: Microbiology
Auburn Univeristy - AL, US,
Expected in 06/2021
Ph.D.: Microbiology
Auburn Univeristy - AL, US,

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Resume Overview

School Attended

  • Univeristy Of Havana
  • Auburn Univeristy
  • Auburn Univeristy

Job Titles Held:

  • Graduate Student Research Assistant
  • Specialist in Microbiology.
  • Reserach Fellow


  • Bachelor of Science
  • Master of Science
  • Ph.D.

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