Scientist Resume Example
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SCIENTIST
Professional Summary
Practiced Scientist and Researcher with history of success across corporate and academic laboratory platforms. Equipped with broad-based experience in laboratory operations, experimental design, bioassay development and quality assurance / control - applying molecular biology and protein engineering techniques in alignment with objectives. Throughout career:
- Collaborated with multidisciplinary teams to advance research in cancer biology and protein engineering.
- Excelled in applying problem-solving, critical thinking, analytical, and planning skills to formulate strategic plans and tackle complex challenges.
- Properly reported, presented, documented, and archived details / results of experiments.
- Collected, analyzed, and applied critical data - supporting attainment of project goals.
Skills
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Work History
Scientist01/2019 － 08/2020Apex SystemsChesapeake , VA
Developed biological sampling reports using molecular biology techniques
- Developed purification protocols successfully for multiple projects.
- Performed Molecular Cloning, PCR amplification, DNA isolation, and sequencing.
- Managed in mammalian cell culture, stable cell line development, CHO / HEK cell expression, and transient cell transfection.
- Prepared cell bank and cryopreservation for master mammalian cell bank
- Delivered protein expression, process optimization, and purification using IMAC, IEC, HIC, Heparin, SEC, and immune affinity purification chromatography.
Collaborated with Assay Development and Process Design groups to improve biologic's purity and activity.
- Designed and optimized ELISA assay conditions for each biologic's purified
- Completed protein biochemistry studies and characterization assays for analysis.
- Designed, executed, interpreted, and presented experimental results
Conducted experiments for product development purposes in laboratory settings.
Developed reports and recommendations regarding research outcomes.
Research Technologist II06/2017 － 01/2019Children's Memorial HospitalNaperville , IL
Conducted molecular cloning and construction for synthetic antibody, antibody conjugation, and antibody engineering.
- Isolation of DNA and molecular cloning for multiple design of synthetic antibody
- Assembled different analogs of synthetic antibody
- Administered expression as well as purification of synthetic antibodies using FPLC (Protein L / SEC).
Designed in vitro cell based bioassay and characterization studies of biosynthetic antibody on cancer cells by ELISA and dose response flow cytometry.
- Designed and executed cell based ELISA assays to screen therapeutic activity of synthetic antibodies
- Designed and optimized dose response flow cytometry assays using cancer cell to screen the binding capacity of synthetic antibodies
- Analyzed different analogs of synthetic antibody
- Teamed with research specialists and scientists to develop and improve processes.
Graduate Research Assistant05/2016 － 05/2017Association Of American Medical CollegesRemote - Washington Dc / Baltimore Area , DC
Provided instruction, training, and support to graduate students focusing on general biotechnology techniques and experiments.
- Worked with faculty and students throughout full lifecycle of experiments, encompassing proper maintenance of resource archives, lab materials, and lab logs.
- Performed research, literature reviews, and data collection / analytics.
Executed qualitative research and quantitative analysis on wide range of data.
- Designed and executed experiments for epithelial mesenchymal transition (EMT) and tyrosine kinase inhibitors (TKI) resistance in non-small lung cancer cell lines (NSCLC).
- Validated novel EMT biomarkers and assisted in validating role of EMT in TKI resistant NSCLC with mutant EGFR.
Research Assistant02/2012 － 01/2013Northwell HealthIslandia , NY
Extracted and purified quercetin from red onion skin using HPLC, sonication, extractor, and dry freezer.
- Planned and initiated projects, conducted literature reviews, and collected as well as analyzed and reported data.
Education
2017Master of Science:University of Illinois - Medical - Medical BiotechnologyCity, USA
- Thesis: “Comparison of Epithelial Mesenchymal Transition Mediated TKI Resistance in Non-Small Lung Cancer Cell Lines Containing Wild Type EGFR and Mutant EGFR"
- Research Project: "Optimization of Concentration for TMPRSS2 Enzyme in Prostate Cancer."
- Membership: Department of Biomedical Sciences Journal Club
- Key Courses: Drug Design and Discovery, Bioethics, Techniques and Processes in Biotechnology, Biotechnology Product Development, Flow Cytometry, Cell Biology, Microbiology, Techniques in Molecular Biology, Techniques in Immunology, Protein Biochemistry, and Biotechnology Biostatistics.
2011Bachelor of Science:Savitribai Phule Pune University - Geology - BiotechnologyCity
- Research: "Optimization of Process Parameters for the Production of Extracellular Lipase by Submerged Fermentation."
- Awards: Indian Government Scholarship, and Best International Student Award.
Affiliations
- Associate Member of American Association for Cancer Research
- Summer Science Program Instructor - UIC, College of Medicine, Rockford
- Fundraiser for Lantuun Dohio
- Member of Cure SMA
- Member of LDS Church Relief Society Group, Chicago, IL
Publications
- Modica, J.A., Iderzorig, T., Mrksich, M. Design and Synthesis of Megamolecule Mimics of a Therapeutic Antibody. J. Am. Chem. Soc. (2020)
- Iderzorig T, Kellen J, Osude C, Puri N. Comparison of EMT mediated tyrosine kinase inhibitor resistance in NSCLC. Biochemical and Biophysical Research Communications, 2018 Feb 5; 496(2):770-777.
- The Shen C, Iderzorig T, Vinay P, Puri N.The role of Epithelial mesenchymal Transition in the Development of Resistance in Non-Small Cell Lung Cancer, . Austin J Lung Cancer Res. 2016; 1(2): 1009 Abstract Authorships
- Iderzorig T., Signh S,. Chhabra G., Puri N. Comparison of Epithelial Mesenchymal Transition mediated TKI resistance in NSCLC cells containing wild type EGFR and mutant type EGFR. ” [Abstract] In Proceedings of the American Association for Cancer Research Annual Meeting 2017, April 02, 2017, Washington, D.C.
- Iderzorig T., Signh S., Foster B., Chhabra G., Puri N. “Mechanism of TKI Resistance and Role of Epithelial Mesenchymal Transition in Non-Small Cell Lung Cancer.” [Abstract] In Proceedings of the Cancer Research Forum of the University of Illinois Cancer Center, October 18, 2016, Chicago, IL.
- Iderzorig T., Chhabra G., Puri N. “Comparison of Epithelial Mesenchymal Transition Mediated TKI Resistance in Non Small Cell Lung Cancer Cell Lines with Wild type EGFR and T790M Mutant EGFR.” [Abstract] In Proceedings of the 17th International Association for the Study of Lung Cancer Chicago Multidisciplinary Symposium in Thoracic Oncology, August 1, 2016, Chicago, IL.
- Iderzorig T., Chhabra G, Puri N. “Epithelial Mesenchymal Transition and Its Role in TKI resistant NSCLC Cell lines.” [Abstract] In Proceedings of the American Association for Cancer Research Annual Meeting 2016, April 18, 2016, New Orleans, LA.
- Iderzorig T., Rastogi I., Chhabra G., Puri N. “Epithelial Mesenchymal Transition and Its Role in TKI Resistant NSCLC Cell Lines. [Abstract] In Proceedings of the 21st Annual Research University of Illinois College of Medicine at Rockford, April 16, 2016, Rockford, IL
Awards
- Thermofisher Scientific Best Basic Science Poster Award 2017
- UIC Department of Biomedical Sciences Travel Award 2017
- UIC Graduate Student Council Travel Awards 2016 / 2017
- UIC Department of Biomedical Sciences Spring 2016 Tuition Waiver Award
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Resume Overview
School Attended
- University of Illinois
- Savitribai Phule Pune University
Job Titles Held:
- Scientist
- Research Technologist II
- Graduate Research Assistant
- Research Assistant
Degrees
- 2017 Master of Science : University of Illinois - Medical - Medical Biotechnology
2011 Bachelor of Science : Savitribai Phule Pune University - Geology - Biotechnology
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