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PhD Candidate Resume Example

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PHD CANDIDATE
Career Overview

Qualified by over six years of research experience in molecular biology, microbiology, immunology and virology. I developed high-throughput gene expression and sequencing assays using RT-qPCR, RNA-seq, SDS-PAGE and 2D gel electrophoresis to establish scientific models in an international, team oriented environment. Additionally, I worked with military and industry leaders to develop novel, PCR-based detection methods (patent pending) for rapid, point-of-care diagnostics of highly infectious diseases (e.g. Plague, Anthrax and Tularemia) .



Summary of Skills
  • Experimental planning and development
  • Next generation sequencing
  • Bioinformatic analysis and genome mining
  • Interpreting and compiling data
  • Scientific and technical writing
  • Molecular techniques
  • Establishing high-throughput assays
  • Organized team player
Experience
Bright FarmsRochelle , ILPhD Candidate06/2009 to Current

Investigated gene expression and characterization of secondary metabolite production in Yersinia pestis. Developed a novel diagnostic technique based on qPCR for the safe detection of highly pathogenic bacteria. Established an institute wide protocol for isolation of nucleic acids and quality control standards for RT-qPCR based on MIQE guidelines. Submitted data to peer reviewed journals and applied for government grants.

Pertinent skills: Assay setup and design using - RT-qPCR, RNA-seq, SDS-PAGE, 2D gels, MALDI-TOF MS, genome mining, bioinformatic analysis, diagnostics, nucleic acid extraction, quantification and quality control.

Weatherford International IncHouma , LAResearch Assistant04/2007 to 06/2009

I worked in a team environment to develop a NeuroAIDS and retroviral drug resistance model by characterizing HIV strains exposed to ART and psychostimulants. Gene targets were studied from brain tissue cultures using RT-qPCR and viral antigen detection was performed from supernatants using ELISA.

Pertinent skills: ELISA, cDNA synthesis, RT-qPCR, cell culture, flow cytometry, antibody staining, fluorescent and confocal microscopy, gel electrophoresis

Education
PhD:Microbiology2014Ludwig Maximilian University, City, Germany

Expected defense date: Jan. 2014.

Private Pilot2013Journey's Aviation Inc., City, State
Bachelor of Science:Biology/Microbiology2008The Ohio State University, City, State
Skills
  • Computer Skills: Bioinformatic analysis using the Galaxy platform, R environment, CLC Workbench, VectorNTI, GenBank, Phyre2, Mauve, ClustalX, ExPASy tools, Ensemble and BLAST. Data plotting software, GIS, Microsoft Office and the use of multiple operating systems (Mac, Windows and Linux)
  • Laboratory Equipment: Agilent 2100 Bioanalyzer, Roche Lightcycler 480, NanoDrop, ELISA plate reader, Alpha Imager, FACS flow cytometer and standard laboratory equipment.
  • Certifications: Certified in the use of radioactive nuclides, BSL-2+/3 laboratories, bloodborne pathogens and animal handling procedures and protocols.
  • Language: I speak Germany fluently as a second language and have six years of formal Spanish education.
Publications

Wieser, A., Schneider, L., Jung, J. and Schubert, S. (2001). MALDI-TOF MS in microbiological diagnostics - identification of microorganisms and beyond. Applied Microbiology and Biotechnology 93, 965-974.


Rakin, A., Schneider, L. and Podladchikova, O. (2012). Hunger for iron: the alternative siderophore iron scavenging systems in highly virulent Yersinia. Frontiers in Cellular and Infection Microbiology 2, 1-7.

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Resume Overview

School Attended

  • Ludwig Maximilian University
  • Journey's Aviation Inc.
  • The Ohio State University

Job Titles Held:

  • PhD Candidate
  • Research Assistant

Degrees

  • PhD : Microbiology 2014
    Private Pilot 2013
    Bachelor of Science : Biology/Microbiology 2008

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