Expected in B.S | Biology Junipero Serra High School, San Diego, CA GPA:
Graduated in top 10% of Class with Honors, lifetime member
of the California Scholastic Federation, Honor’s French Award, Honor’s English Award, Presidential Fitness, Award, Lettered Varsity Tennis Team.
Skills
Hematology
Laboratory Safety and Universal precaution; proper specimen collection including phlebotomy, physiologic
factors affecting test results; proper use of specimen collection equipment for venipuncture including the use of
anticoagulant and preservative (i.e., EDTA tube); skin puncture site determination and its proper sample
collection; preparation of blood smears; QA in specimen collection as well as rejection of improperly collected
samples.
Able to perform both automated and manual blood cell testing including QC, routine testing of RBC and
Reticulocytes, and examination of the peripheral blood smear including a manual differential count of WBC,
diagnosis of leukemic and other abnormal cells including bone marrow aspirates; common blood parasite
identification such as malarial cells, babesia, trypanosomes, as well as micro-filarial cells; perform manual
counts on body fluids including CSF, amniotic peritoneal, pleural and pericardial, seminal (Semen Analysis), as
well as synovial fluid for crystal identification (i.e., uric acid crystal for gout); familiarity with common critical
values in the laboratory and common criteria for RBC morphology review, differential review, and pathology
review.
Able to perform instrument maintenance and troubleshooting; calibrate instrument biannually or if the need
arises as indicated; Levy-Jennings Westgard Multi Rule as part of the Quality Management Program to observe
for trending patterns, shifts, or any outliers that may trigger either a random error or a systematic error on the
instrument.
Coagulation
Identification of properly collected sample in a sodium citrate tube for routine PT, APTT, Fibrinogen, D-dimer
testing (i.e., DIC, DVT, and PE), as well as other factor assay testing for send outs; use of properly prepared QC
material for testing; mean of the normal study for proper calculation of the INR for Coumadin patients and
monitoring of patients that have been given heparin on the ward.
Troubleshooting, Maintenance, Calibration, Levy-Jennings QC data submission on a monthly basis for Peer
Review, and much more.
Urinalysis
Verification of QC in control prior to acceptance of properly submitted urine sample (i.e., either random or first
morning specimen or 24 hour specimen) from the ward, testing of specimen sample, including identification of
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both normal and abnormal formed elements, under the microscope or in the instrument; familiarity of
interfering substances in urine testing that would invalidated test results.
Daily, weekly, monthly instrument maintenance for proper instrument function; instrument calibration as
needed and as required by the College of American Pathologist; Levy-Jennings monitoring for analytes or tests
with numerical values such as the pH or the specific gravity to monitor instrument for both random error and
systematic error.
Serology
RPR testing for Syphilis, hCG (both urine/blood/serum) qualitative testing using urine test kits, Fetal Fibronectin
testing, ICON Mono Spot for IM testing, Sickle Cell Screen, Rapid Strep A Testing, Influenza A and B testing, RSV
testing, H. pylori, HIV ½ Screen or any other minor rapid testing kit, including the collection of Hepatitis Panel or
any exotic test that require the assistance of a reference laboratory.
Immunohematology
Perform basic type and screen and type and cross using Gel technology, emergency release units and
autologous transfusion units; positive DAT testing and Immune Mediated Hemolysis, perinatal issues in transfusion
practice including HDN, familiarity with Kleihauer-Bethke acid elution test, neonatal and pediatric transfusion
practice, platelet and granulocyte antigens and antibodies, identification of antibodies to red cell antigens
(both forward and reverse blood grouping and typing of both major and minor blood groups); storage,
monitoring, pre-transfusion processing and distribution of blood components, as well as infectious disease
screening and cord blood testing; familiarity with temperature monitoring system in both the refrigerator and
water bath within the blood bank section; facilities work environment and safety, as well as regulatory issues in
Immunohematology.
Clinical Chemistry
Perform pre-analytical, analytical and post-analytical testing, as well as post lab analysis verification for possible
errors prior to the release of the result; Clinical evaluation of methods, establishment and use of reference
values in the laboratory; proper maintenance of the analyzer to help in the evaluation of random versus
systematic error of the instruments; application of the Westgard Multi Rule as part of the instrument
troubleshooting protocol in monitoring possible shift, trending patterns in QC materials, establishment of
recommended ranges for QC materials, stability of the QC material, calibration from lot to lot between QC to
monitor variations, as well as semi-annual calibrations for compliance of CAP, as the regulating body; familiarity
with critical values of different analytes, as well as the proper laboratory protocol and documentation
regarding call back to the provider; knowledge of possible interfering substance in the specimen sample, drug
of abuse testing protocol, panel testing to identify certain, disease states, clinical toxicology and toxic metals
testing, as well as molecular diagnostic and genetic testing.
Microbiology
Familiarity with the proper collection technique in microbiology using the aseptic technique; quality controls;
culture and isolate microorganisms using the different type of streaking methods; able to identify
microorganisms using multiple identification techniques such as the microscope slide techniques (i.e., negative
staining, smear preparation, simple and capsular staining, Gram staining as well as acid fast staining, etc.); Set
UP of positive blood culture bottle and ID of organisms present on Gram Stain. Safety, Instrument, Maintenance
and Trouble shooting capability.
Molecular Biology
Theoretical knowledge in Molecular Science including Nucleic Acid Chemistry, basic Molecular Theory and
Genetics, as well as biochemical reagents; familiarity with molecular techniques, including separation and
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detection, nucleic acid amplification and DNA sequence analysis; application of molecular testing in infectious
diseases, oncology, and genetics; knowledge in laboratory operations including guidelines, quality assurance
and contamination.