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Team Leader - Development, Clinical Biochemistry Resume Example

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DM
TEAM LEADER - DEVELOPMENT, CLINICAL BIOCHEMISTRY
Summary

Analytical chemist application scientist, with over 7 years of experience in CLIA laboratory practices, adept at testing small molecules with systematic and organized approach. Proficient in multiple analytical techniques, documentation requirements, project management and writing technical papers. Proven method development skill set producing numerous CLIA and CAP compliant assays in conjunction to CLSI guidelines.

Skills
  • LC-MS/MS
  • GC-MS
  • HPLC-UV/VIS
  • RT-qPCR
  • ELISA
  • Immunohistochemistry (IHC)
Experience
07/2020 - CurrentSr. Laboratory Technical ConsultantUnited States Agency for International Development (USAID) | Honduras, El Salvador
  • Performed onsite evaluation of laboratory processes as related to COVID-19 testing, based on needs assessment implemented a capacity strengthening strategy in alignment with USAID program directives
  • Increased COVID-19 RT-qPCR testing capacity of the Honduras National Virology Laboratory from 800 to 2500+ samples per day
  • Implemented QC guidelines and management systems.
  • Developed and executed training workshop to enhance laboratory staff knowledge and practices as related to COVID-19 testing
  • Standardized laboratory procedures with implementation of SOPs and workflow guides, introducing CLSI guideline standards across the laboratory
  • Improved general laboratory capacity through an optimization of the workflow of the laboratory, in conjunction with strategic acquisition of equipment
  • In conjunction with fellow technical advisors, developed an Access based LIMS system to help with sample and data management for COVID-19 testing
07/2019 - 07/2020Team Leader - Development, Clinical BiochemistryThe Cleveland Clinic Foundation | Cleveland, OH
  • Developed new LC-MS/MS assay utilizing ortholognal online-SPE sample clean up coupled with hybrid analytical column chemistries in a multiplex set up to develop a 47-analyte assay for toxicology screening and quantification of opiates, amphetamines, benzodiazepines, barbiturates, and custom synthetic drugs
  • Provided leadership and direction for development team employees, supervising activities to drive productivity and efficiency resulting in the development, validation, and transfer of 2 new assays in 6 months
  • Implemented new research method to explore ideal sample clean up and chromatography separation through cheminformatics, saving time in development by identifying ideal extraction and chromatographic chemistries based on target analyte structure
  • Taught laboratory technicians LC-MS/MS fundamentals and developed their skill set to take a leading role in the validation and transfer of individual projects
  • Oversaw the contract research groups development of various assays for automated chemistry analyzers, RT-qPCR, and as well as manual ELISA platforms
06/2015 - 05/2019Chemist - Research Fellow (ORISE)Center for Disease Control and Prevention (CDC); Tobacco and Volatiles Branch | Atlanta, GA
  • Perform national level biomonitoring of metabolites indicative of VOC exposure as part of the NHANES and FDA PATH studies (+15,000 samples analyzed per year) utilizing a UPLC-ESI-MS/MS methodology (Waters UPLC and AB Sciex MS)
  • Perform statistical analysis and conducted artificial neural network analysis using JMP
  • Conduct method validation of all new assays and revalidate existing methods to new internal guidance for accuracy, precision and stability testing
  • Overview of the proficiency testing (PT) for the VOC metabolite laboratory in accordance with scheduled timeline and requirements per CLIA and state regulations
  • Review chromatography integration of fellow chemists to determine the fitness of each analysis before the data release
  • Wrote correction action plans (CAP) for resolution of identified errors in the analysis
  • Provide training for new hires in laboratory and data analysis functions
  • Update standard operating procedure (SOP) in accordance with ongoing refinements to maintain readiness and compliance for CLIA audits
06/2013 - 06/2015Toxicology Clinical TechnologistGenova Diagnostics | Duluth, GAanalyteanalyteorthogonalortholognalortholognalortholognalorthogonal
  • Utilize CLIA compliant methodology to detect and quantify for the presence of volatile organic compounds in whole blood via GCMS, phthalates, and parabens in urine via LCMS, organic acids in serum via LCMS, and organophosphate pesticide metabolites in urine via GCMS.
  • Conducted RT-qPCR screening for Cytomegalovirus.
  • Develop new assay methodology to increase sample recovery, time efficiency, and cut reagent cost
  • Implement new screening procedure to isolate contamination of organic solvents from vendors sample tubes and solvents
  • Analyze and review patient chromatography data via MassLynx, Empower and ChemStation
  • Prepare calibrators, internal standards, quality control samples, and other test reagents
  • Review and release of specimen data
  • Responsible for the training of all new technicians in the LCMS/General Chemistry Dept
Education and Training
2013Bachelor of Science: Neuroscience, Analytical Chemistry
Georgia State University | Atlanta, GA
Ph.D.: Pharmaceutical Sciences
Mercer University | Atlanta, GA

Spring 2017-Spring 2019, withdrew under medical hardship

Publications
  • Biomonitoring of Xylene Urinary Metabolites across the U.S. population: NHANES 2005-2006, 2011-2012, and 2013-2014. Víctor R. De Jesús, Daniel F. Milan, Yoo Young, Deepak Bhandari, , Kevin S. Murnane, and Benjamin C. Blount. Manuscript submitted to journal Biomarkers, 10/13/2020.
  • Correlation of Creatinine‐ and Specific Gravity‐Normalized Free & Glucuronidated Urine Cannabinoid Concentrations Following Smoked, Vaporized and Oral Cannabis in Frequent and Occasional Cannabis Users. Marilyn A. Huestis, Benjamin C. Blount, Daniel F. Milan, Matthew N. Newmeyer, Jennifer Schroeder, Michael L. Smith. Drug Testing Analysis, Feb 12 2019, DOI 10.1002/dta.2576
  • Urinary acrylonitrile metabolite concentrations before and after smoked, vaporized, and oral cannabis in frequent and occasional cannabis users. David L. Ashley, Víctor R. De Jesús, Osama A. Abulseoud, Marilyn A. Huestis, Daniel F. Milan and Benjamin C. Int. J. Environ. Res. Public Health 2020, 17(18), 6438
Activities and Honors
  • 1st place poster presentations at 2017 Current Trends in Mass Spectrometry Conference
  • Research Fellowship award from the Oak Ridge Institute for Science and Engineering (ORISE)
  • Ronald E. McNair Undergraduate Researcher Fellowship award
  • AP Scholar with distinction
Software Skills
  • Analyst, MultiQuant - AB Sciex
  • TraceFinder, FreeStyle - Thermo Fisher
  • MassLynx, QuanLynx, Empower- Waters
  • JMP statistics
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Resume Overview

Companies Worked For:

  • United States Agency for International Development (USAID)
  • The Cleveland Clinic Foundation
  • Center for Disease Control and Prevention (CDC); Tobacco and Volatiles Branch
  • Genova Diagnostics

School Attended

  • Georgia State University
  • Mercer University

Job Titles Held:

  • Sr. Laboratory Technical Consultant
  • Team Leader - Development, Clinical Biochemistry
  • Chemist - Research Fellow (ORISE)
  • Toxicology Clinical Technologist

Degrees

  • 2013 Bachelor of Science : Neuroscience, Analytical Chemistry
    Ph.D. : Pharmaceutical Sciences

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