Experienced in generating genetically engineered mice, working with nearly 30 labs, at the MIT Core Transgenic Facility.
Scope of experience includes inception of the core transgenic facility to successful and reproducible generation of genetically engineered animal models.
In vivo generation of mice with key MIT/Whitehead Institute labs, including: Hynes lab, Sharps lab, Tonegawa lab, Jacks lab, Lees lab & Page lab
University of Massachusetts at Amherst1982Bachelor of Sciencewildlife biology and zoology (developmental biology)
Alpha Zeta Honors; Statistics for Life Science Honors (integrative approach employing calculus, computer science & statistics)
University of CambridgeAdvanced Level Certificate (A Levels Full Certificate): English, Math, Physics, Chemistry and Biology
Massachusetts Institute of TechnologyJanuary 1989 to CurrentTransgenic Facility Manager/Microinjection Specialist
Creation and development of Transgenic Facility, Center for Cancer Research/Koch Institute.
Hired by Professor Sharp (Director) & Professor Ruley (Faculty) at Center for Cancer Research based on my 4 year research project at Harvard Medical School in assisted reproduction and transgenics, honor graduate studies in immunology and genetics as well as attendant publication on assisted reproduction & learning/memory Creation of transgenic animal models.
Generation of genetically engineered mice using: embryo recovery pronuclear and intracytoplasmic microinjection; DNA or RNA/CRISPR microinjection blastocyst microinjection employing embryonic stem cells manual intracytoplasmic microinjection (ICSI) Electrofusion for generation of tetraploid embryos Nuclear transfer (ES Cell transfer) and tetraploid technology embryo culture embryo transfer: infundibulum (oviduct) and uterine transfers rederivation embryo transfer for mouse lines IVF cryobiology - sperm and egg preservation Transgenic colony management - overall colony structure and function establishment (donor and recipient/surrogate mice setup and management) Congenics Mouse Colony for breeding & ear snips PCR genotyping Hormone priming Zebra fish enucleation and nuclear transfer - jumpstart project with Nick Carter, Hopkins lab Center for Cancer Research Production of microinstruments using electromechanics toward embryo microsurgery: embryo microinjection and reconstitution Postdoctoral training and tutorials toward creation of transgenic mice: primarily training postdocs on 3-month rotation in the transgenic facility: MIT Department of Comparative Medicine; training and tutorials for undergrad, grad, and visiting students and scientists at MIT - Department of Comparative Medicine.
The transgenic facility routinely supports nearly 30 labs at MIT utilizing the facility for: generation of transgenic mice/genetically engineered mice for gene function evaluation and assessment using diploid and tetraploid blastocyst microinjection; pronuclear microinjection rederivation of already existing transgenic lines as Assisted Reproduction (ART): in vitro fertilization (IVF) and embryo transfer (ET); embryo or sperm preservation and cryobiology: embryo freezing (vitrification ) or sperm freezing Database management including statistical analysis.
Applied BiotechnologyJanuary 1989 to July 1989Research Associate Cambridge, MA
Production of polyvalent recombinant vaccine using vaccinia virus; assessment of antigen immunogenicity; animal models; Elisa technology.
Harvard Medical SchoolJanuary 1985 to January 1988
Laboratory for Human Reproduction and Reproductive Biology Research Staff, Dr John Biggers Laboratory Dr Biggers is a celebrated pioneer in mammalian embryo culture and embryo transfer with Dr Anne McLaren at University College London, 1958; Oxford and Cambridge Universities; their research work laid the foundation for advances vitro fertilization (IVF) as well as, reproduction, developmental biology and manipulation of mammalian embryos, including transgenics.
Central participation in research, development and optimization of reproductive technologies for creation of mammalian genetically identical twins using the in vivo mouse model Embryo microsurgery and embryo reconstitution: zona evacuation and nuclear (blastomere/stem cell) transfer; blastomere (stem cell) genomic totipotency evaluation Optimization of embryo reconstitution and microsurgery, pronuclear microinjection; embryo culture as well as embryo transfer technologies toward endangered species recovery and creation of animal models for gene function evaluation and assessment Optimization of transgenic technology, including participation in the development of KSOM culture medium Database management including statistical analysis.
Nyika National Park & MichiruJanuary 1982 to January 1984Mountain Park, Malawi
Government of Malawi, Ministry of Natural Resources and the Environment National Wildlife Park Scientist/Biologist Terms of office included: Park administration/management Research - gene pool evaluation and preservation, especially threatened and endangered species such as cheetah, rhino and the African Elephant National conservation/environmental policy derivation and promulgation as sustainable development Attendant public education toward mitigation of environmental degradation or gene pool erosion; overall biodiversity preservation, including threatened and endangered species; preservation of the earth's life support systems integrity - the biosphere, hydrosphere, geosphere and atmosphere.
Publication: weekly national columnist - conservation and environmental science, education and policy promulgation- sustainable development in Southern Africa (approx.
15 countries) for Malawi's preeminent national English newspaper, "Daily Times of Malawi" Reporting to the principal secretary and cabinet minister of Natural Resources and Environment as well as the president of the nation, whenever required, as coordinator of the Michiru Mountain Park steering committee and conservation authority.
Laser Assisted IVF and Modified ICSI Markedly Improve Efficiency of IVF in C57BL/6 mice John Mkandawire, Tony Chavarria, Peimin Qi, Alan Discua, Alex Viana, Susan Erdman, James G. Fox, Kirk J. Maurer 2008 American Association For Laboratory Animal Science Poster Laser-Assisted In Vitro Fertilization Facilitates Fertilization of Vitrified-Warmed C57BL/6 Mouse Oocytes with Fresh and Frozen-Thawed Spermatozoa, Producing Live Pups Stephanie E. Woods, Peimin Qi, Elizabeth Rosalia, Tony Chavarria, Allan Discua, John Mkandawire, James G. Fox, Alexis García Published: March 11, 2014DOI: 10.1371/journal.PLOS ONE.0091892 Comparison of Tetraploid Blastocyst Microinjection of Outbred Crl:CD1(ICR), Hybrid B6D2F1/Tac, and Inbred C57BL/6NTac Embryos for Generation of Mice Derived from Embryonic Stem Cells Sharron M Kirchain, Alison M Hayward, John M Mkandawire, Peimin Qi, and Aurora A Burd Comparative Medicine 2008 Vol 58, No 2 Development and phenotypic variability of genetically identical half mouse embryos VIRGINIA E. PAPAIOANNOU 1, JOHN MKANDAWIRE 2, 3, and JOHN D. BIGGERS 2 Development 106, 817-827 (1989) ACKNOWLEDGEMENTS We wish to thank John Mkandawire for dedicated technical assistance. Journal of in Vitro Fertilization and Embryo Transfer, Vol. 8, No. 6, 1991 Submitted: May 17, 1991; Accepted: June 14, 1991