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STUDENT INTERN
Professional Summary
Ph.D. in chemical engineering and biotechnology from UC Davis. Working as an Engineer at UMass Amherst. Protein production. I enhanced two-fold the production of antibodies and pharmaceutical proteins with new methods I developed and invented three patents describing those methods. Protein purification. Increased reproducibility and lifespan of a disposable filtration system four-fold by designing purification experiments and revising protocols. Computational methods. Fundamentally changed the way scientists understand circadian biology by using MATLAB, mathematical model simulations, and Design of Experiment (DoE) principles.
Core Qualifications
| gene design, transfection, cloning, strain selection, PCR (QIAGEN kits) Cell culture: Grown mammalian (HEK293), plant, bacterial, and yeast (P. pastoris) cell cultures using shake flasks, 10L (New Brunswick), and pilot scale (Applikon) bioreactors Purification: AKTA chromatography with UNICORN, HPLC, ultrafiltration, tangential flow filtration, precipitation, centrifugation, dialysis, IMAC, size exclusion (SEC), ion exchange, and Protein A Biochemical assays: ELISA, quantitative protein assays, spectrophotometry, electrophoresis, immunostaining Computational skills: coding, data analysis, mathematical modeling, JMP, MATLAB, C++, Python CMC: Process testing, technology transfer, technical report review, certification, and analysis |
Experience
Student Intern
January 2014 to CurrentCatholic Health Initiative
January 2014 to CurrentCatholic Health Initiative
- Design of Experiment and hypothesis testing to contribute to the discovery of fundamentally new ways in which neurons in the brain interact.
- This was done by comparing mathematical models with experimental results.
- Work was performed in collaboration with Prof.
- Michael Henson at UMass and Prof.
- David Welsh and Dr.
- Takako Noguchi at UCSD.
- Data analysis from complex data sets compiled from mouse brain slice images yielded insights for crafting circadian biology’s most comprehensive model.
- Published in the Journal of Theoretical Biology and presented at invited seminars and conferences.
- Researcher at UC Davis 1/4/2009 – 12/20/2013 Process development to achieve 2-fold yield increases and 100% purity with new extraction methods for downstream recovery of recombinant proteins from tobacco leaf tissue.
- This played a key role in the production of antibodies, vaccine components, and pharmaceutical proteins with Prof.
- Karen McDonald.
- Expression, extraction, and purification of antibodies from tobacco using my novel methods achieved better product homogeneity and glycosylation profiles: an important step towards human use.
- Led independent research study that formed the basis of intellectual property granted to the University by achieving the advances in protein extraction and purification techniques.
- Responsible for compliance with government regulatory agencies to ensure biological and chemical use compliance.
- Training and maintenance, as lab manager, of AKTA chromatography and other equipment.
- Supervisory role for three undergraduate students, challenging them to perform independent research, write honors theses, and launch their careers in the biotech industry.
- Published in BioMed Research International, presented at conferences and advised external partners on how to enhance protein recovery in their own research using my inventions.
- at Lawrence Livermore National Laboratory 07/08/2013 – 09/20/2013 Optimized and modeled a renewable biofuels system and found the ideal operating conditions for achieving highest theoretical efficiency.
- Worked collaboratively with engineers and regulatory teams during planned construction of a municipal scale waste treatment center.
- My contribution included calculating theoretical mass and energy balances with engineering software.
Student Intern
June 2006Catholic Health Initiative
June 2006Catholic Health Initiative
January 2007
- Solved a reproducibility problem afflicting one of Pall’s ultrafiltration membranes by applying models of protein purification through porous materials.
- Developed new protocols to increase up to four-fold the volume that can flow through these filters and documented these achievements in white papers.
Education
Ph.D : Chemical Engineering, Designated Emphasis Biotechnology, 03/20/2014University of California － CityChemical Engineering, Designated Emphasis Biotechnology
B.S : Bioengineering, 05/19/2008Lehigh University － City, StateBioengineering
Graduate
Interests
U.S. Patent Application No. 14/547,022. Published on May 21, 2015, entitled, “Treatment of Leaf Tissue for the Recovery of Interstitial Recombinant Proteins by the Application of Cell Wall Degrading Enzymes.” U.S. Patent Application No. 14/052,617. Published on April 17, 2014, entitled, “Apoplast Wash Fluid Recovery for Improved Recombinant Endoglucanase Production in Tobacco Leaves.” U.S. Patent Application No. 13/186,423. Published on February 23, 2012, entitled, "Plant based Production of Heterologous Proteins.” SELECTED PUBLICATIONS Kingsbury NJ, Taylor SR, and Henson MA (2016) Inhibitory and Excitatory Networks Balance Cell Coupling in the Suprachiasmatic Nucleus: a Modeling Approach. Journal of Theoretical Biology (accepted). Kingsbury NJ and McDonald KA (2014) Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method. BioMed Research International. Kingsbury NJ and McDonald, KA (2013) Selective Recovery from Interstitial Spaces of Leaf Tissue. 2013 AIChE Annual Meeting Conference Proceedings. http://www3.aiche.org/proceedings/content/Annual-2013/extended-abstracts/P325861.pdf Noguchi T, Leise TL, Kingsbury NJ, Diemer T, Wang LL, Henson MA, and Welsh DK. Calcium Circadian Rhythmicity in the Suprachiasmatic Nucleus: Cell Autonomy and Network Modulation (in preparation). Kingsbury NJ and McDonald, KA. Increased Expression from Tobacco Leaves by Implementing Daily Cycles of Vacuum Infiltration and Centrifugation to Recover Apoplast Wash Fluid (in preparation).
Skills
Biology, C++, Cell culture, conferences, Data analysis, Design of Experiment, dialysis, downstream, ELISA, government, HPLC, IMAC, intellectual property, materials, MATLAB, exchange, modeling, Molecular biology, novel, PCR, Process development, coding, protocols, Python, Research, Researcher, seminars, Supervisory, white papers, vaccine
Additional Information
- PATENTS U.S. Patent Application No. 14/547,022. Published on May 21, 2015, entitled, “Treatment of Leaf Tissue for the Recovery of Interstitial Recombinant Proteins by the Application of Cell Wall Degrading Enzymes.” U.S. Patent Application No. 14/052,617. Published on April 17, 2014, entitled, “Apoplast Wash Fluid Recovery for Improved Recombinant Endoglucanase Production in Tobacco Leaves.” U.S. Patent Application No. 13/186,423. Published on February 23, 2012, entitled, "Plant based Production of Heterologous Proteins.” SELECTED PUBLICATIONS Kingsbury NJ, Taylor SR, and Henson MA (2016) Inhibitory and Excitatory Networks Balance Cell Coupling in the Suprachiasmatic Nucleus: a Modeling Approach. Journal of Theoretical Biology (accepted). Kingsbury NJ and McDonald KA (2014) Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method. BioMed Research International. Kingsbury NJ and McDonald, KA (2013) Selective Recovery from Interstitial Spaces of Leaf Tissue. 2013 AIChE Annual Meeting Conference Proceedings. http://www3.aiche.org/proceedings/content/Annual-2013/extended-abstracts/P325861.pdf Noguchi T, Leise TL, Kingsbury NJ, Diemer T, Wang LL, Henson MA, and Welsh DK. Calcium Circadian Rhythmicity in the Suprachiasmatic Nucleus: Cell Autonomy and Network Modulation (in preparation). Kingsbury NJ and McDonald, KA. Increased Expression from Tobacco Leaves by Implementing Daily Cycles of Vacuum Infiltration and Centrifugation to Recover Apoplast Wash Fluid (in preparation).
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Resume Overview
School Attended
- University of California
- Lehigh University
Job Titles Held:
- Student Intern
Degrees
- Ph.D : Chemical Engineering, Designated Emphasis Biotechnology , 03/20/2014
B.S : Bioengineering , 05/19/2008
Graduate
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