|gene design, transfection, cloning, strain selection, PCR (QIAGEN kits) Cell culture: Grown mammalian (HEK293), plant, bacterial, and yeast (P. pastoris) cell cultures using shake flasks, 10L (New Brunswick), and pilot scale (Applikon) bioreactors Purification: AKTA chromatography with UNICORN, HPLC, ultrafiltration, tangential flow filtration, precipitation, centrifugation, dialysis, IMAC, size exclusion (SEC), ion exchange, and Protein A Biochemical assays: ELISA, quantitative protein assays, spectrophotometry, electrophoresis, immunostaining Computational skills: coding, data analysis, mathematical modeling, JMP, MATLAB, C++, Python CMC: Process testing, technology transfer, technical report review, certification, and analysis|
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