Research Scientist with comprehensive cell and molecular biology expertise. Experienced in all aspects of biochemical and cell-based assays from gene/protein expression in eukaryotic and prokaryotic systems to cellular regulation and function. Proficient in experiment planning, execution, analysis and presentation. Hands on infectious diseases and cell biology project management experience. Managerial and Industrial Experiences: Responsibilities included generation of proposal, cell based assay development, SOPs, scope of the project, timelines and initial budget. Also responsible for technical and procedural trouble shooting, analysis and presentation of data. Managed external vendors, CRO's, projects and trained scientist and technicians. Experience working in a GMP/GLP setting and managing BSL2 Laboratory. Quality Control/Assurance/Safety officer for implementing and maintaining quality standards in all aspects of the development and production process. Ability to coordinate and lead multiple projects simultaneously. Budgeting, develop operational and scheduling laboratory members. Assay development: Mammalian expression systems, cell based assays, primary T-cell culture, cryopreservation of cultures, in vitro assays, secretion assays. Infrared dye and fluorescent based assays. Familiarity with CyAn and Accuri 6 Flow cytometers, FACS. Cell biology: Mammalian cell culture (CHO, THP1), Stem cells, iPSC, stable cell line, transfection/retrovirus, siRNA transfection, electroporartion, clone selection, high throughput cell culture, fluorescent and confocal microscopy, immunohistochemistry (IHC) and immunocytochemistry (ICC), image processing and analysis. Fermentation: Natural and recombinant bacteria, yeast/bacterial genetics, production optimization, enzyme production. Protein purification: Ammonium sulfate precipitation, ultrafiltration (UF), dialysis, size exclusion (SEC), ion exchange (IEX) and affinity chromatography for membrane and soluble proteins, pull-down assays. Familiarity with HPAE-PAD, Mass spectrometry (LC-MS, MALDI-TOF MS). Biochemistry and molecular biology: Molecular cloning, DNA and RNA isolation, cDNA synthesis, recombinant protein expression and purification (His, Myc, GST-tag), protein turnover/metabolism, polyacrylamide gel electrophoresis, 2-D gel electrophoresis, Western blotting, immunoprecipitation (magnetic and agarose beads), spectrophotometry, ELISA, enzyme assays, PCR, Real-time qPCR.
DNASTAR (Lasergene, Seqman), ImageJ, Zeiss LSM Imaging, Adobe Photoshop, MetaMorph image analysis, QCapture, Slidebook 5.0 and MS Office (Word, Excel, Power Point)
Senior Scientist Cell Biology July 2013 to CurrentBell Biosystems － San Francisco, CA.
Supervised and planned assay development/quantification, optimization and validation of mammalian cell labeling for cell therapy applications.
Maintained and handled bacterial and mammalian cell cultures on daily basis.
Purified and analyzed bacterial toxins/proteins and tested them using in-vitro cell culture assay.
Generated antibodies and developed biochemical and cell-based assays that significantly increased the productivity and product development (now an integral part of the product kit).
Managed preclinical studies for MRI cell tracking assessment project for Agilent Technologies, Inc.
Handled multiple projects, completed, prepared and presented project reports.
Post-Doctoral Associate March 2012 to July 2013NJH － Denver, CO.
Supervised regulation of T cell signaling studies in cell based and in vivo models (rodents) of chronic and acute asthma.
Planned and supervised research on regulator of intracellular signaling molecules for lead discovery in cell based models.
Established proteins purification protocols and performed immunoprecipitation (IP) and Co-IP to decipher cell signaling pathways.
Developed cell based and cell free protein-protein and receptor-ligand binding assays (TCR).
Isolated, purified, cultured CD4+ T cell, and performed CD4+ T cell proliferation assay using CFSE, multicolor flow cytometry, FACS, subcloned genes in bacterial and mammalian expression vectors, bicistronic retroviral vectors, generated retroviral particles, conducted transfections, T cell infection, T cell stimulation and differentiation assays.
Research Scientist August 2009 to March 2012University of Texas Medical Branch － Galveston, TX.
Managed multiple projects and trained scientists and technicians.
Developed molecular host-pathogen interaction assay models for virulence factor and vaccine target discovery in bacterial infection models.
Performed molecular cloning, recombinant and native protein expression, purification and analysis, proteomics, two-dimensional gel electrophoresis.
Identified and characterized native and recombinant proteins using techniques including chromatography and mass spectrometry (MALDI-TOF, GC-, LC-MS/MS, and HPAE-PAD).
Identified and characterized toxins related to Repeats-in-Toxins (RTX) for discovery of lead therapeutic targets for human ehrlichioses in cell based secretion assay models.
Developed in vitro cell based assays for phosphorylation, acetylation, glycosylation, ubiquitination.
Established effective liaison and fostered inter and intra-institutional collaborations.
Post : Infectious Diseases/Cell BiologyUniversity of Texas Medical Branch － Galveston, TXInfectious Diseases/Cell Biology
Ph.D : Biology/BiochemistryJawaharlal Nehru University － New Delhi, IndiaBiology/Biochemistry
More than 10 published articles and 20 presentations in national and international conferences.
Adobe Photoshop, cell culture, conferences, GC, image, Imaging, IP, Excel, MS Office, Power Point, Word, optimization, pathogen, presentations, product development, protocols, research, therapy, vaccine, validation, articles
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Companies Worked For:
University of Texas Medical Branch
University of Texas Medical Branch
Jawaharlal Nehru University
Job Titles Held:
Senior Scientist Cell Biology
Post : Infectious Diseases/Cell Biology Ph.D : Biology/Biochemistry More than 10 published articles and 20 presentations in national and international conferences.
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