Research Scientist with comprehensive cell and molecular biology expertise. Experienced in all aspects of biochemical and cell-based assays from gene/protein expression in eukaryotic and prokaryotic systems to cellular regulation and function. Proficient in experiment planning, execution, analysis and presentation. Hands on infectious diseases and cell biology project management experience. Managerial and Industrial Experiences: Responsibilities included generation of proposal, cell based assay development, SOPs, scope of the project, timelines and initial budget. Also responsible for technical and procedural trouble shooting, analysis and presentation of data. Managed external vendors, CRO's, projects and trained scientist and technicians. Experience working in a GMP/GLP setting and managing BSL2 Laboratory. Quality Control/Assurance/Safety officer for implementing and maintaining quality standards in all aspects of the development and production process. Ability to coordinate and lead multiple projects simultaneously. Budgeting, develop operational and scheduling laboratory members. Assay development: Mammalian expression systems, cell based assays, primary T-cell culture, cryopreservation of cultures, in vitro assays, secretion assays. Infrared dye and fluorescent based assays. Familiarity with CyAn and Accuri 6 Flow cytometers, FACS. Cell biology: Mammalian cell culture (CHO, THP1), Stem cells, iPSC, stable cell line, transfection/retrovirus, siRNA transfection, electroporartion, clone selection, high throughput cell culture, fluorescent and confocal microscopy, immunohistochemistry (IHC) and immunocytochemistry (ICC), image processing and analysis. Fermentation: Natural and recombinant bacteria, yeast/bacterial genetics, production optimization, enzyme production. Protein purification: Ammonium sulfate precipitation, ultrafiltration (UF), dialysis, size exclusion (SEC), ion exchange (IEX) and affinity chromatography for membrane and soluble proteins, pull-down assays. Familiarity with HPAE-PAD, Mass spectrometry (LC-MS, MALDI-TOF MS). Biochemistry and molecular biology: Molecular cloning, DNA and RNA isolation, cDNA synthesis, recombinant protein expression and purification (His, Myc, GST-tag), protein turnover/metabolism, polyacrylamide gel electrophoresis, 2-D gel electrophoresis, Western blotting, immunoprecipitation (magnetic and agarose beads), spectrophotometry, ELISA, enzyme assays, PCR, Real-time qPCR.
