Microbiology: QC Analytical Testing for LBP,Aerobic and Anaerobic bacterial culturing using aseptic techniques, Culture media preparation, Optimization of media and fermentation conditions, Working with anaerobic work stations, Gram staining, Colony morphology, Cell morphology, Microbial assay of antibiotics, Microscopy, Total cell counts, MALDI-TOF, Flow cytometry, FACS and biochemical ID (i.e. Vitek-MS), Lyophilization and freeze-drying of bacteria, Live/dead screening using bacterial viability kit, Bacterial counting and enumeration, Bacterial endotoxin measurements using LAL assay, Viable titer (CFU), Bioactivity assays, Water activity, Stability testing, ELISA, SDS-PAGE, Western blot, HPLC, Moisture analysis, Bioburden (USP 61 and USP 62).
Molecular Biology: qPCR, ddPCR, RT-PCR, Sequencing (NGS, 16S, WGS, MicroSeq 500, WGBS, Shotgun and Nanopore), DNA/RNA extraction, Nanodrop, Qubit, cDNA preparation, Genotyping, library preparation for sequencing, Terminal restriction fragment length polymorphism (TRFLP), Cloning using PCR and restriction enzymes, Predicted microbial functional analysis (PICRUSt).
Biochemistry: Karl Fischer titration, Metabolomics (LC-MS), Gas chromatography (GC), Protein extraction and purification, Lipid extraction and triglyceride quantification, In vivo gut permeability assay using FITC-dextran
Immunology: Immunocytochemistry, Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, MagPix Luminex, Luciferase assay.
Microscopy: Light microscope equipped with color camera system, Automated immunofluorescent microscopy, Confocal laser scanning microscope with fully automated confocal imaging system, Inverted light microscope with easy-to-use software and high-resolution cameras and Automated Digital Microscopy
Cell biology: Cell lines/primary cell culture (media Preparation, media changes, trypsinizing the cells, Freezing down cells and Resuspending cells from frozen stocks, Cell growth curve), Transfection and Transduction of cell lines, Angiogenesis in vitro/in vivo, Cell adhesion and chemotactic migration assay, Cell proliferation/apoptosis/differentiation assays.
Computer skills: Microbiome data analysis (computational tools and statistical methods),CloudLIMS-based lab management, Electronic Research Notebook (ERN), Advanced in Microsoft tools, Adobe Acrobat/Photoshop, Specialized image analysis software (ImageJ) and Statistical analysis software (GraphPad Prism, R programming, IPA, MicrobiomeAnalyst, MetaboAnalyst, Galaxy, SIMCA, XLSTAT, PAST, QIIME, Gephi, IPA and Cytoscape).
Investigated effects of transitioning unit-based transport team to external service on ICU, wrote IRB protocol and authored professional scientific paper for publishing
Higher Performance in Microbiology and Biochemistry
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