Scientist January 2014 to CurrentIndiana University
Bacterial pathogenesis, gene regulation, microbial physiology and genetics signal transduction, microbial stress responses Recombinant protein expression in bacteria and mammalian cells, protein purification/engineering Characterization and functional analysis of various proteins, Biochemical and cell-based assays advanced molecular biology, bacterial genetics, strain engineering Served a team leader of research projects to design and construct experiments.
Supervising junior scientists and graduate students and assisting in resolving their technical difficulties.
Develop, optimize, and exploit new methods and protocols.
Published in peer-reviewed journals Laboratory of Prof.
Taeok Bae Study of the molecular mechanisms underlying bacterial disease in Staphylococcus aureus (MRSA) Exploration of gene regulation and development of anti-infective therapeutics Conceived and executed original scientific research on gene regulation of the SaeRS two-component regulatory system by human immune system (human neutrophil peptides, HNPs) in Staphylococcus aureus (MRSA).
Discovered the mechanism by which the extracytoplasmic linker peptide controls the kinase activity of the sensor protein SaeS in response to host signals and is critical for S.
aureus's virulence in mice.
Determined the neutrophil protein calprotectin enhances the activity of the SaeRS two component system and increases murine mortality.
Studied the role of cardiolipin in staphylococcal cell membrane in activation of various two-component systems.
Exploited an anti-infective therapeutic agent and studies of various multi-drug resistant S.
Investigated infectious diseases caused by Staphylococcus aureus using mouse model and human neutrophils.
Postdoctoral Research Associate January 2011 to January 2013University of Florida/Emerging Pathogens Institute
Laboratory of Prof.
Kwang Cheol Jeong Investigated host-pathogen interaction and biological roles of effector proteins in Enterohaemorrhagic E.
Discovered the mechanism of antibacterial activity of chitosan microparticles against various pathogenic bacteria and implications on the treatment of infectious diseases.
Characterized antibacterial resistant microorganisms isolated from farm animals and conducted genomic screening to find genes responsible for antibacterial resistance.
Yale University/Howard Hughes Medical Institute Washington University in St.
Louis/ Howard Hughes Medical Institute.
Postdoctoral Research Associate January 2006 to January 2011
Laboratory of Prof.
Groisman Led, designed, and executed research projects to investigate the molecular mechanisms of two-component signal transduction systems and gene regulation in Salmonella typhimurium.
Engineered bacterial strains, generated mutant alleles, designed and developed biochemical and cell-based assays to examine biological roles of histidine kinases in controlling gene expression.
Designed, overproduced and purified mutant proteins to examine their biochemical activities using radioisotopes (in vivo and in vitro kinase and phosphatase, nucleotide binding studies, EMSA, transcription assays) Discovered and characterized a mechanism of intrinsic negative feedback whereby a change in the opposing biochemical activities of a bifunctional histidine kinase results in differential gene expression, which has implications for the diverse cellular events that depends on the enzyme's function.
Determined a new mechanism of gene regulation whereby a transcriptional factor H-NS controls the timing and level of expression of horizontally acquired genes.
PhD Biological Sciences (Molecular Microbiology) 2005 MS Biological Sciences (Molecular Microbiology) 2001 Dong-Eui University BSc Biological Sciences (Microbiology) 1999 Seoul National University
2009) SoxRS-mediated LPS modification enhances resistance against multiple drugs in Escherichia coli.
Yeo, and JH.
2008) Oxidant-responsive induction of the suf operon encoding a Fe-S assembly system through Fur and IscR in Escherichia coli.
Lee, and JH.
2006) IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins.
featured in Faculty of 1000 Biology).
2004) Induction of the sufA operon encoding Fe-S assembly proteins by superoxide generators and hydrogen peroxide: involvement of OxyR, IHF and an unidentified oxidant-responsive factor.
2003) A reducing system of the superoxide sensor SoxR in Escherichia coli.
2; 22 (11):2614-2622.
featured in Faculty of 1000 Biology).
equal contribution) f JH.
2003) Regulation of the sufABCDSE operon by Fur.
Manuscripts submitted or in preparation for publication: Q.Liu*, HS, Cho*, WS.
Yeo*, and T.
The extracytoplasmic linker peptide of the sensor protein SaeS tunes the kinase activity required for staphylococcal virulence in response to host signals" (* equal contribution, submitted to PLoS Pathogens, under revision).
Chazin, and T.
Calprotectin can increase murine mortality in a sae-dependent and independent manners during infections by Staphylococcus aureus" (submitted to PLoS Pathogens).
Yeo, and KC.
Phenotypic comparison of the non-LEE effector families EspR, EspX, and EspY from enterohemorrhagic Escherichia coli O157:H7." WS.
Yeo, and EA.
Dissection of a sensor histidine kinase pinpoints residues disconnecting kinase and phosphatase activities." SELECTED PRESENTATIONS Midwest Microbial Pathogenesis Conference (poster) Chicago, 2014 The extracellular linker peptide of the sensor protein SaeS tunes the kinase activity required for virulence in Staphlyococcus aureus in response to human neutrophil peptide 1 American Society for Microbiology (poster) Boston, MA 2014 Lipoproteins play a key role in the human neutrophil peptide (HNP)-mediated activation of the SaeRS two- component system in Staphylococcus aureus Animal Molecular and Cellular Biology Research Symposium (oral) St.
Augustine, FL 2013 Paralogous non-LEE effectors of EHEC target disparate loci in host cells and cause cell damage Emerging Pathogens Institute Research Day (poster) Gainesville, FL 2012 A study of effector proteins secreted by enterohemorrhagic Escherichia coli O157:H7 Microbiology 12th Annual Symposium (poster) West Haven, CT 2010 Intrinsic phosphatase activity of bifunctional sensor kinases governs activation surge Gordon Research Conference "Microbial Stress Response" (poster) South Hadley, MA 2010 Switching between kinase and phosphatase states of bifunctional histidine sensor kinases governs activation surge in two-component systems Washington University Infectious Diseases/Basic Microbiological Mechanisms Symposium (oral) St.
Louis, MO 2009 How bifunctional sensor proteins switch between kinase and phosphatase form.
I. Zwir, WS. Yeo, D. Shin, T. Latifi, H. Huang, and EA. Groisman. (2014) Bacterial nucleoid-associated protein uncouples transcription levels from transcription timing. mBio. 7; 5(5). pii: e01485-14.
SJ. Jeon, M. Oh, WS. Yeo, KN. Galvão, and KC. Jeong. (2014) Underlying mechanism of antimicrobial activity of chitosan microparticles and implications for the treatment of infectious diseases. PLoS ONE 9(3): e92723. doi:10.1371/journal.pone.0092723.
WS. Yeo, I. Zwir, H. Huang, D. Shin, A. Kato, and EA. Groisman. (2012) Intrinsic negative feedback governs activation surge in two-component regulatory systems. Mol Cell. 10; 45 (3):409-21.
WS. Yeo, MJ. Seo, MJ. Kim, HH. Lee, BW. Kang, JU. Park, YH. Choi, YK. Jeong. (2011) Biochemical analysis of a fibrinolytic enzyme purified from Bacillus subtilis strain A1. J Microbiol. 49 (3):376-80.
acid, Bae, Basic, BSc, functional, IP, team leader, exchange, MS Office, optimization, PAGE, PCR, PhD, Prism, protocols, Reporter, research, scientific, Supervising, transcription, Western blotting
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Companies Worked For:
University of Florida/Emerging Pathogens Institute
Seoul National University
Job Titles Held:
Postdoctoral Research Associate
PhD Biological Sciences (Molecular Microbiology) 2005 MS Biological Sciences (Molecular Microbiology) 2001 Dong-Eui University BSc Biological Sciences (Microbiology) 1999
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