- Molecular Microbiology
- Manipulation of pathogenic bacteria (E.coli O157:H7, Salmonella typhimurium, Staphylococcus aureus)
- Microbial assays (MIC, susceptibility, zone of inhibition, disc assay, agar plate diffusion, LD50)
- Reporter gene fusions, gene knockouts, recombination, phage transduction, transformation, strain engineering
- DNA/RNA isolation and manipulation, gene cloning, PCR, DNA sequencing, SDS-PAGE, qRT-PCR, Western blotting
- Genomic library construction and screening , mutagenesis, recombinant protein expression and optimization
- Microscopy, cell isolation and culture, screening of multi-drug resistance bacteria
- Biochemistry
- Protein purification including use of FPLC, column chromatography (affinity, ion-exchange, SEC), IP, pull-down
- Enzyme assays and kinetics, nucleic acid biochemistry, protein secretion assay, SCAM
- Biochemical assays using radioisotopes (in vivo/in vitro kinase and phosphatase, ligand binding, in vitro transcription, S1 mapping, EMSA, DNaseI footprinting)
- Isolation and characterization of protein complexes, chimera and protein variant design, mutagenesis
- Handling analytical equipment
- Bioinformatics, Cell Biology and others
- Prism, Origin, MS Office, Unicorn, PyMol
- Microbiologica l/aseptic techniques (BSL-2 agents)
- Basic bioinformatics analyses (Vector NTI, BioEdit, BLAST, ClusterX/W)
- Animal experiment (mouse genotyping, mouse model for survival, bacterial counts)
- Mammalian cell culture, transfection, immunostaining, immunofluorescence, cell line-based enzyme assays (cytotoxicity)
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