Ph.D : Biochemistry and Molecular Biology, 2006Colorado State University - Colorado, USAGPA: GPA: 3.91Biochemistry and Molecular Biology GPA: 3.91
Bachelor Degree : Medicine, 1994Colorado State University - Colorado, USAMedicine
Faculty of Medicine, Zagazig University Zagazig, Egypt
Experimental design Primer design
Developed HPLC and UPLC methods Experienced in Maldi Mass spec and Biotyper for bacterial and yeast identification Analyzed small molecules and peptides MRM after collecting data from Orbitrap and TQS Mass spectrometer Successfully published ten articles and reviews in peer-reviewed journals and presented research at international scientific meeting Excellent skills in teamwork and collaboration Great commitment and organization skills.
Nucleic Acids Isolation Real time PCR
In-vitro transcription, Polyadenylation and Sanger Sequencing Deadenylation Expert in DNA data analysis Knowledgeable about cloning and subcloning Immunofluorescence assay expertise Protein expression and purification Library preparation for Next Generation Sequencing MALDI Mass Spectrometer HPLC and UPLC Northern, Southern and Western blotting Method development and data analysis for Mass Developed and immortalized mammalian cell lines Spectrometer (Orbitrap - CGMS - TQS) siRNA Mascot and Scafold Customer Service Python and Linux.
Research Scientist January 2011 to December 2015Proteomics and Metabolomics Facility PMF
Assistant Professor of Medical Biochemistry October 2008 to February 2011Colorado State University
Postdoctoral Researcher October 2006 to September 2008Faculty of Medicine at Zagazig University - Zagazig, Egypt
Skills Colorado State University - Fort Collins, CO
Experienced in molecular biology techniques, stable cell line generation, protein purification and characterization.
Conducted cell line development, clone screening, clone selection and recombinant protein expression.
Experienced with Sanger DNA Sequencing, Fragment analysis and operation of Genetic Analyzer 3130-XL instruments Analyzed high throughput data for DNA and proteins (Mascot and Scaffold) Effectively prepared next generation sequencing library.
Department of Microbiology Immunology and Pathology
siRNA meeting. April 14 - 19, Keystone, Colorado, United States2004 Poster presentation at the Experimental Biology Meeting. April 11-15, San Diego, California, United States 2003 Annual poster presentation, Colorado State University 2002-2005 Annual Cell and Molecular Biology poster competition, Colorado State University 2002-2009 Publications 1. Ashraf S.A El-Sayed, Safwat Abdel-Azeim, Hend M. Ibrahim, Marwa M Yassin, Salah E Abdelghany, Sadik Esener, Gul Shad Ali (2015): Biochemical stability and molecular dynamic characterization of Aspergillus fumigatus cystathionine -lyase in response to various reaction effectors. Enzyme and Microbial Technology, Volume 81, December 2015, Pages 31- 46 2. Ashraf SA El-Sayed, Abdallaa E Hassan, Marwa A Yassin, Hend MM Ibrahim and Asmaa M Hassan (2015): Biochemical and Pharmacokinetic Properties of Aspergillus flavipes Glutathione-Homocystine Transhydrogenase of Unique Affinity for Homocystine Reduction using GSH as Hydrogen Donor Medicinal Chemistry, May 17, 2015 3. Ashraf S.A. El-Sayed, Marwa A. Yassin and Hend Ibrahim (2014): Co-Immobilization of L-Methioninase and Glutamate Dehydrogenase: Novel Approach for L-Homoalanine Synthesis. Biotechnol Appl Biochem. 2014 Oct 2. 4. Yousry Abo Elmajd, Hend Ibrahim, Nermin Raafat and Yousry Abdel Mawla (2014): Evaluating the Interleukin 17 serum level in psoriatic patients from Eastern Egyptian population. Indian Journal of Applied Research, Vol 4, July 2014. 5. Ashraf S.A. El-Sayed, Hend Ibrahim and Sitohy MZ. (2014): Co-immobilization of PEGylated Aspergillus flavipes L-methioninase with glutamate dehydrogenase: A novel catalytically stable anticancer consortium. Enzyme Microb Technol. 54:59-69, January 2014 6. Fumihiko Sagawa, Hend Ibrahim, Angela Morrison, Carol J. Wilusz and Jeffrey Wilusz (2011): Nucleophosmin Deposition during mRNA 3' End Processing Influences Poly (A) Tail Length and mRNA Export. EMBO, 5;30(19):3994-4005 7. Ebtesam Ibrahim Ahmad, Tarek Ali M Elgohary and Hend M. M. Ibrahim (2011): Naturally occurring T regulatory cells, Interleukins 10 and 12 in the pathogenesis of Idiopathic Warm Autoimmune Hemolytic Anemia. Journal of Investigational Allergology and Clinical Immunology, Vol. 21(4): 297-304. 8. Ibrahim H, Wilusz J, Wilusz CJ (2008): RNA recognition by 3'-to-5' exonucleases: The substrate perspective. Biochim Biophys Acta. 1779 (4): 256-265 9. Ibrahim H, Lee YJ, Curthoys NP (2007): Renal response to metabolic acidosis: role of mRNA stabilization. Kidney Int. 73 (1): 11-18. 10. Schroeder JM, Ibrahim H, Taylor L, Curthoys NP (2006): Role of deadenylation and AUF1 binding in the pH-responsive stabilization of glutaminase mRNA. Am J Physiol Renal Physiol. 290 (3): 733-740 11. Porter LD, Ibrahim H, Taylor L, Curthoys NP (2002): Complexity and species variation of the kidney-type glutaminase gene. Physiol Genomics. 9 (3): 157-166.
Memberships and Affiliations
Research scientist with expertise in data collection and analysis of DNA, proteins and small molecules. Ten years experienced in advanced molecular biology techniques (DNA and RNA biology). Accomplished in generating and applying new protocols and technologies. Experienced in design and analysis for qPCR based projects. Self motivated, hard worker with great attention to details and independent work ethic. Ability to multi-task and meet deadlines in a fast-paced, customer-oriented environment. Effective written and oral communication skills in English. Seeking a fulfilling, full time position and willing to relocate.
Customer Service, data analysis, DNA, Experimental design, HPLC, Linux, Mascot, molecular biology, Next, Python, transcription, Western blotting