Innovative Research Chemist with 7 years' experience in clinical and toxicological research and a proven track
record of designing and spearheading toxicological and pharmacokinetic bioassays. Expertise in managing all
aspects of research studies while devising new methods to improve throughput and maintain high quality
standards. Accomplished in making significant contributions to regulatory and basic scientific research in high
priority areas for the FDA. Seeking to bring talent and expertise to a renowned institution in a Senior Scientist
Clinical and Toxicological Analysis Quantitative Mass Spectrometry
Study Design and Management Process Optimization
Good Laboratory Practices Mentorship and Leadership
2016 NCTR/FDA Special Act Award for exceptional service as a member of NCTR Summer Student
2013 NCTR/FDA Outstanding Service Award for outstanding analytical support relating to toxicity of
melamine and cyanuric acid
2011 FDA Group Recognition Award as member of Melamine and Cyanuric Acid Toxicity Investigative
January 2012 to CurrentResearch Chemist / Staff Fellow Jefferson, AR
Plan and manage toxicological and pharmacokinetic bioassays, analyzing all formulation-related and
biological samples and performing non-compartmental analysis on data.
Direct study activities, including formulation mixing and supply purchasing to maintain uninterrupted
continuation of study while working collaboratively with study director, animal technicians, diet
preparation, and other team members to achieve study objectives and advance organizational mission.
Uncover opportunities to improve workflow and maintain high quality standards by optimizing
formulation mixing protocols, performing stability testing, testing gavage pump accuracy, certifying
dose concentrations, and certifying test articles using LC-UV, LC-MS, LC-MS/MS, and NMR.
Develop sample preparation and LC-MS, LC-MS/MS, and HPLC-UV methods to conduct quantitative
and qualitative analyses on compounds such as drugs, metabolites, DNA adducts, cosmetology products,
and food products, supporting ongoing toxicological studies conducted at NCTR for the FDA.
Perform complex quantitative analyses using stable isotope dilution LC-ESI/MS methods and qualitative
analyses using LC/MS, GC/MS, NMR, LC-UV, and other techniques.
January 2010 to Current
Conceptualized, coordinated, and oversaw studies examining nephrotoxicity following co-exposure to
melamine and cyanuric acid in adult rats (sub-chronic study), in neonatal rats (treatment PND 1-21) and
during recovery study after 90-day co-exposure to melamine and cyanuric acid.
Conducted PK study evaluating pharmacokinetics of oseltamivir and its pharmacologically active
metabolite oseltamivir carboxylate in non-pregnant rhesus macaques and during three trimesters of their
Designed high-throughput LC-MS/MS method to analyze oseltamivir and oseltamivir carboxylate in
rhesus macaque plasma, enabling analysis of 150 samples per day and 3,000 samples within one month.
Developed simple "one-pot" liquid-liquid extraction sample preparation and hydrophilic interaction
liquid chromatography isotope dilution mass spectrometry method to quantify nicotine, cotinine, and
two tobacco-specific nitrosamines (NNN and NNK) in small blood serum volume, allowing for
high-throughput preparation of 100 samples per hour without automation; publication in peer review
Realized and performed experiments to elucidate oxidative mechanism underlying cleavage of diphenyl
ether bond of triclosan during incubations with human liver microsomes (CYPs) in isotopically-enriched
water, using LC-MS and LC-MS/MS to perform qualitative analyses; published findings in Archives of
Devised and validated ultra-sensitive LC-MS/MS method to quantify 4-hydroxy-1-(3-pyridyl)-
1-butanone (HPB) in HPB-releasing DNA adducts derived from tobacco-specific nitrosamines.
National Center For Toxicological Research (NCTR) / Food and Drug Administration (FDA).
Oak Ridge Institute for Science and Education ORISEJanuary 2010 to January 2012Postdoctoral Fellow TN
Performed stability testing, dose certifications, gavage pump accuracy testing, and test article analysis
for two toxicological bioassays following good laboratory practice (GLP) regulations, including
preparing standard operating procedures and conducting all chemistry analyses.
Co-designed, managed, and provided analytical support to two studies addressing nephrotoxicity from
co-exposure to melamine and cyanuric acid in F344 rats - sub-chronic study in adult rats and in newborn
rats from PND 1 to 21 (weaning).
Developed HPLC-UV method to quantify melamine and cyanuric acid in 0.1% and 0.5% carboxymethyl
cellulose dosing formulations.
Optimized ball-milling process of melamine and cyanuric acid using laser-diffraction particle size
Synthesized highly-selective derivatization reagent for alkyne-bearing compounds, and designed sample
preparation procedures and LC-MS/MS methods.
Research Assistant at the University of Basilicata in Italy, Research Assistant at the University of Texas at
Arlington, and Research and Teaching Assistant and Researcher at Charles University in Prague in the Czech
Education and Training
University of Arkansas for Medical Sciences2014Graduate Certificate in Regulatory Science
Coursework in FDA Regulations, Toxicology in Public Health, Clinical Trial Design and Management, Risk
Assessment, and Good Clinical, Laboratory, and Manufacturing Practices
Charles University2010Doctor of Philosophy: Analytical ChemistryPrague, Czech RepublicAnalytical Chemistry Dissertation: Study of enantioseparation of selected substituted binaphthyls
Charles University2005Master of Science: Analytical ChemistryPrague, Czech RepublicAnalytical Chemistry
Activities and Honors
Oak Ridge Institute for Science and Education (ORISE) and NCTR/FDA
2015 to Current
Mentor and Committee Member, Summer Student Research Program, Jefferson, AR
El Dorado Education Foundation
2012 to Current
Speaker, Girls Learning About Math and Science (GLAMS), El Dorado, AR
Society of Toxicology Member, 2016 to Current
American Society for Mass Spectrometry Member, 2010 to Current
Reviewer, Journal of Agricultural and Food Chemistry (ACS Publications), 2017 to Current
Reviewer, Current Analytical Chemistry (Bentham Science), 2016 to Current
Internal Reviewer, National Center For Toxicological Research, 2013 to Current
L. Loukotková, L. S. VonTungeln, M. Vandanlingham, G. Gamboa da Costa. A simple and highly
sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of nicotine, cotinine, NNN,
and NNK in serum samples. In peer review for submission to J. Chromatogr. B.
Wu, P. Chitranshi, L. Loukotková, G. Gamboa da Costa, F. A. Beland, J. Zhang, J.-L. Fang.
Cytochrome P450-mediated metabolism of triclosan attenuates its cytotoxicity in hepatic cells. Arch.
Toxicol. 91:6 (2017) 2405-2423.
R.L. Sprando, R. Reimschuessel, C.B. Stine, T. Black, N. Olejnik, M. Scott, Z. Keltner, O. Bandele, M.
Ferguson, S.M. Nemser, A. Tkachenko, E. Evans, T. Crosby, K. Woodling, L. Loukotková and G.
Gamboa da Costa. Timing and route of exposure affects crystal formation in melamine and cyanuric
exposed male and female rats: Gavage vs. Feeding., Food Chem. Toxicol. 50:12 (2012) 4389-4397.
H. Qiu, L. Loukotková, P. Sun, E. Tesarová, Z. Bosáková, D. W. Armstrong: Cyclofructan 6 based
stationary phases for hydrophilic interaction liquid chromatography. J. of Chromatogr. A, 1218 (2011)
L. Loukotková, E. Tesarová, Z. Bosáková, D. W. Armstrong: Comparison of enantioseparation of
substituted binaphthyls on cyclodextrin-, cellulose- and synthetic polymer-based chiral stationary
phases. J. Sep. Sci. 33 (2010) 1244-1254.
V. Pacáková, L. Loukotková, Z. Bosáková, K. Stulík: Analysis for estrogens as environmental
pollutants - A review. J. Sep. Sci. 32 (2009) 867-882.
Comprehensive list of additional publications (4) available upon request.
L. Loukotková and G. Gamboa da Costa. A simple and highly sensitive UPLC-ESI-MS/MS
methodology for the simultaneous quantification of nicotine, cotinine, NNN, and NNK in serum
samples. Poster presentation, 65th ASMS Conference on Mass Spectrometry and Allied Topics,
Indianapolis, IN, June 2017
M. McNamara, L. Loukotková, P. Chitranshi, G. Gamboa da Costa. Edible Oil Fingerprinting Using
Direct Infusion ESI-MS and Principal Component Analysis. Poster presentation, 253rd ACS National
Meeting & Exposition in San Francisco, CA, April 2017
Linda. VonTungeln, L. Loukotková, R. Reimschuessel, C. B. Stine, G. Olson, F. A. Beland, and G.
Gamboa da Costa. Combined nephrotoxicity of melamine and cyanuric acid - a recovery study. Poster
presentation, 56th SOT Annual meeting & ToxExpo, Baltimore, MD, March 2017
C.S. Silva, C.-B. Fang, P. Porter-Gill, L. Loukotková, L. VonTungeln, G. Gamboa da Costa, and L.
Camacho. Kidney miRNA and Gene Expression Changes during the Recovery from Nephrotoxicity
Induced by a 90-Day Co-Exposure to Melamine and Cyanuric Acid in Rats. Poster presentation, 56th
SOT Annual meeting & ToxExpo, Baltimore, MD, March 2017
J. Fang, Y. Wu, L. Loukotková, P. Chitranshi, G. Gamboa da Costa, F. A. Beland. Cytotoxicity of
12-Sulfoxynevirapine. Poster presentation, 56th SOT Annual meeting & ToxExpo, Baltimore, MD,
Comprehensive list of additional conference presentations (21) available upon request.
Scientific Software: MassLynx (Waters Corp.), Excalibur and Chromeleon (Thermo Scientific), TopSpin
(Bruker Corp.), ACD Sketch and ACD LC Simulator, WinNonlin, PK Solutions, SigmaPlot, XLStat
Other Software: Microsoft Office Suite, Scientific Databases
Oak Ridge Institute for Science and Education ORISE
University of Arkansas for Medical Sciences
Job Titles Held:
Research Chemist / Staff Fellow
Graduate Certificate in Regulatory Science Coursework in FDA Regulations, Toxicology in Public Health, Clinical Trial Design and Management, Risk
Assessment, and Good Clinical, Laboratory, and Manufacturing Practices Doctor of Philosophy : Analytical Chemistry Master of Science : Analytical Chemistry
Create a job alert for [job role title] at [location].