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Research Chemist / Staff Fellow Resume Example

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RESEARCH CHEMIST / STAFF FELLOW
Summary
Innovative Research Chemist with 7 years' experience in clinical and toxicological research and a proven track record of designing and spearheading toxicological and pharmacokinetic bioassays. Expertise in managing all aspects of research studies while devising new methods to improve throughput and maintain high quality standards. Accomplished in making significant contributions to regulatory and basic scientific research in high priority areas for the FDA. Seeking to bring talent and expertise to a renowned institution in a Senior Scientist role. Core Qualifications Clinical and Toxicological Analysis Quantitative Mass Spectrometry Study Design and Management Process Optimization Good Laboratory Practices Mentorship and Leadership
Accomplishments
  • 2016 NCTR/FDA Special Act Award for exceptional service as a member of NCTR Summer Student Research Committee 2013 NCTR/FDA Outstanding Service Award for outstanding analytical support relating to toxicity of melamine and cyanuric acid 2011 FDA Group Recognition Award as member of Melamine and Cyanuric Acid Toxicity Investigative Group.
Experience
January 2012 to CurrentResearch Chemist / Staff Fellow
Rockford , IL
  • Plan and manage toxicological and pharmacokinetic bioassays, analyzing all formulation-related and biological samples and performing non-compartmental analysis on data.
  • Direct study activities, including formulation mixing and supply purchasing to maintain uninterrupted continuation of study while working collaboratively with study director, animal technicians, diet preparation, and other team members to achieve study objectives and advance organizational mission.
  • Uncover opportunities to improve workflow and maintain high quality standards by optimizing formulation mixing protocols, performing stability testing, testing gavage pump accuracy, certifying dose concentrations, and certifying test articles using LC-UV, LC-MS, LC-MS/MS, and NMR.
  • Develop sample preparation and LC-MS, LC-MS/MS, and HPLC-UV methods to conduct quantitative and qualitative analyses on compounds such as drugs, metabolites, DNA adducts, cosmetology products, and food products, supporting ongoing toxicological studies conducted at NCTR for the FDA.
  • Perform complex quantitative analyses using stable isotope dilution LC-ESI/MS methods and qualitative analyses using LC/MS, GC/MS, NMR, LC-UV, and other techniques.
January 2010 to Current
  • Conceptualized, coordinated, and oversaw studies examining nephrotoxicity following co-exposure to melamine and cyanuric acid in adult rats (sub-chronic study), in neonatal rats (treatment PND 1-21) and during recovery study after 90-day co-exposure to melamine and cyanuric acid.
  • Conducted PK study evaluating pharmacokinetics of oseltamivir and its pharmacologically active metabolite oseltamivir carboxylate in non-pregnant rhesus macaques and during three trimesters of their pregnancy.
  • Designed high-throughput LC-MS/MS method to analyze oseltamivir and oseltamivir carboxylate in rhesus macaque plasma, enabling analysis of 150 samples per day and 3,000 samples within one month.
  • Developed simple "one-pot" liquid-liquid extraction sample preparation and hydrophilic interaction liquid chromatography isotope dilution mass spectrometry method to quantify nicotine, cotinine, and two tobacco-specific nitrosamines (NNN and NNK) in small blood serum volume, allowing for high-throughput preparation of 100 samples per hour without automation; publication in peer review process.
  • Realized and performed experiments to elucidate oxidative mechanism underlying cleavage of diphenyl ether bond of triclosan during incubations with human liver microsomes (CYPs) in isotopically-enriched water, using LC-MS and LC-MS/MS to perform qualitative analyses; published findings in Archives of Toxicology.
  • Devised and validated ultra-sensitive LC-MS/MS method to quantify 4-hydroxy-1-(3-pyridyl)- 1-butanone (HPB) in HPB-releasing DNA adducts derived from tobacco-specific nitrosamines.
  • Experience National Center For Toxicological Research (NCTR) / Food and Drug Administration (FDA).
Oak Ridge Institute For Science And Education ORISEJanuary 2010 to January 2012Postdoctoral Fellow
MA
  • Performed stability testing, dose certifications, gavage pump accuracy testing, and test article analysis for two toxicological bioassays following good laboratory practice (GLP) regulations, including preparing standard operating procedures and conducting all chemistry analyses.
  • Co-designed, managed, and provided analytical support to two studies addressing nephrotoxicity from co-exposure to melamine and cyanuric acid in F344 rats -sub-chronic study in adult rats and in newborn rats from PND 1 to 21 (weaning).
  • Developed HPLC-UV method to quantify melamine and cyanuric acid in 0.1% and 0.5% carboxymethyl cellulose dosing formulations.
  • Optimized ball-milling process of melamine and cyanuric acid using laser-diffraction particle size analyzer.
  • Synthesized highly-selective derivatization reagent for alkyne-bearing compounds, and designed sample preparation procedures and LC-MS/MS methods.
  • Research Assistant at the University of Basilicata in Italy, Research Assistant at the University of Texas at Arlington, and Research and Teaching Assistant and Researcher at Charles University in Prague in the Czech Republic.
Education and Training
University of Arkansas for Medical Sciences2014Graduate Certificate in Regulatory Science
Coursework in FDA Regulations, Toxicology in Public Health, Clinical Trial Design and Management, Risk Assessment, and Good Clinical, Laboratory, and Manufacturing Practices
Charles University2010Doctor of Philosophy: Analytical ChemistryCity, Czech RepublicAnalytical Chemistry Dissertation: Study of enantioseparation of selected substituted binaphthyls
Charles University2005Master of Science: Analytical ChemistryCity, Czech RepublicAnalytical Chemistry
Activities and Honors
Oak Ridge Institute for Science and Education (ORISE) and NCTR/FDA 2015 to Current Mentor and Committee Member, Summer Student Research Program, Jefferson, AR El Dorado Education Foundation 2012 to Current Speaker, Girls Learning About Math and Science (GLAMS), El Dorado, AR Professional Affiliations Society of Toxicology Member, 2016 to Current American Society for Mass Spectrometry Member, 2010 to Current
Publications
Reviewer, Journal of Agricultural and Food Chemistry (ACS Publications), 2017 to Current Reviewer, Current Analytical Chemistry (Bentham Science), 2016 to Current Internal Reviewer, National Center For Toxicological Research, 2013 to Current Publications L. Loukotková, L. S. VonTungeln, M. Vandanlingham, G. Gamboa da Costa. A simple and highly sensitive UPLC-ESI-MS/MS method for the simultaneous quantification of nicotine, cotinine, NNN, and NNK in serum samples. In peer review for submission to J. Chromatogr. B. Wu, P. Chitranshi, L. Loukotková, G. Gamboa da Costa, F. A. Beland, J. Zhang, J.-L. Fang. Cytochrome P450-mediated metabolism of triclosan attenuates its cytotoxicity in hepatic cells. Arch. Toxicol. 91:6 (2017) 2405-2423. R.L. Sprando, R. Reimschuessel, C.B. Stine, T. Black, N. Olejnik, M. Scott, Z. Keltner, O. Bandele, M. Ferguson, S.M. Nemser, A. Tkachenko, E. Evans, T. Crosby, K. Woodling, L. Loukotková and G. Gamboa da Costa. Timing and route of exposure affects crystal formation in melamine and cyanuric exposed male and female rats: Gavage vs. Feeding., Food Chem. Toxicol. 50:12 (2012) 4389-4397. H. Qiu, L. Loukotková, P. Sun, E. Tesarová, Z. Bosáková, D. W. Armstrong: Cyclofructan 6 based stationary phases for hydrophilic interaction liquid chromatography. J. of Chromatogr. A, 1218 (2011) 270-279. L. Loukotková, E. Tesarová, Z. Bosáková, D. W. Armstrong: Comparison of enantioseparation of substituted binaphthyls on cyclodextrin-, cellulose- and synthetic polymer-based chiral stationary phases. J. Sep. Sci. 33 (2010) 1244-1254. V. Pacáková, L. Loukotková, Z. Bosáková, K. Stulík: Analysis for estrogens as environmental pollutants - A review. J. Sep. Sci. 32 (2009) 867-882. Comprehensive list of additional publications (4) available upon request. Conference Presentations L. Loukotková and G. Gamboa da Costa. A simple and highly sensitive UPLC-ESI-MS/MS methodology for the simultaneous quantification of nicotine, cotinine, NNN, and NNK in serum samples. Poster presentation, 65th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 2017 M. McNamara, L. Loukotková, P. Chitranshi, G. Gamboa da Costa. Edible Oil Fingerprinting Using Direct Infusion ESI-MS and Principal Component Analysis. Poster presentation, 253rd ACS National Meeting & Exposition in San Francisco, CA, April 2017 Linda. VonTungeln, L. Loukotková, R. Reimschuessel, C. B. Stine, G. Olson, F. A. Beland, and G. Gamboa da Costa. Combined nephrotoxicity of melamine and cyanuric acid - a recovery study. Poster presentation, 56th SOT Annual meeting & ToxExpo, Baltimore, MD, March 2017 C.S. Silva, C.-B. Fang, P. Porter-Gill, L. Loukotková, L. VonTungeln, G. Gamboa da Costa, and L. Camacho. Kidney miRNA and Gene Expression Changes during the Recovery from Nephrotoxicity Induced by a 90-Day Co-Exposure to Melamine and Cyanuric Acid in Rats. Poster presentation, 56th SOT Annual meeting & ToxExpo, Baltimore, MD, March 2017 J. Fang, Y. Wu, L. Loukotková, P. Chitranshi, G. Gamboa da Costa, F. A. Beland. Cytotoxicity of 12-Sulfoxynevirapine. Poster presentation, 56th SOT Annual meeting & ToxExpo, Baltimore, MD, March 2017 Comprehensive list of additional conference presentations (21) available upon request.
Skills
Scientific Software: MassLynx (Waters Corp.), Excalibur and Chromeleon (Thermo Scientific), TopSpin (Bruker Corp.), ACD Sketch and ACD LC Simulator, WinNonlin, PK Solutions, SigmaPlot, XLStat Other Software: Microsoft Office Suite, Scientific Databases
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Resume Overview

School Attended

  • University of Arkansas for Medical Sciences
  • Charles University

Job Titles Held:

  • Research Chemist / Staff Fellow
  • Postdoctoral Fellow

Degrees

  • Graduate Certificate in Regulatory Science
    Coursework in FDA Regulations, Toxicology in Public Health, Clinical Trial Design and Management, Risk Assessment, and Good Clinical, Laboratory, and Manufacturing Practices
    Doctor of Philosophy : Analytical Chemistry
    Master of Science : Analytical Chemistry

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