Seeking a position that will enable me to bridge my research skills and knowledge with translational science. Accomplished independent Protein Biochemist and Molecular Biologist with a strong portfolio of orchestrating success in projects in Cell and Molecular Biology of Emerging and Infectious Diseases and other projects
Protein expression using bacterial and mammalian platforms;
Protein denaturation and refolding using redox strategies
Protein purification techniques:, affinity, ion, hydrophobic, size exclusion chromatography and HPLC
Protein characterization techniques: electrophoresis, electro-elution , electroblotting N-terminal sequencing,peptide mass fingerprinting (MALDI-TOF) , collision-induced fragmentation (LC MS/MS)., of proteins, Southern and Western blotting, Antibody purification by Protein A and Protein G beads
Protein-Protein and RNA-Protein interaction techniques: TAP-TAG (tandem affinity purification) fusion protein generation, chemically crosslinked proteins, exonuclease and RNA-binding assays, in vitro transcription, 5' and 3' end labeling, and gel purification of the labeled RNAs. radiolabelling of proteins, immunoprecipitation, co-immunoprecipitation, ELISA .
Cell Biology techniques: Transfection and stable cell line generation in mammalian cells, immunofluorescence assays, FACS assay,.
Research Associate Uniformed Services University of Health Sciences
Research Participant (ORISE), FDA, SilverSpring.
Elucidated the role of Babesia microti Apical Membrane Antigen1(AMA1) protein in human RBC invasion and its potential as a vaccine candidate Elucidated the role of the Yeast Sm-Like (LSM) proteins in mRNA decay.
Discovered that the Lsm1p-7p complex influences 3* to 5' exosome mediated mRNA decay.
Research Associate Post Doctoral Fellow Team leader Uniformed Services University of Health Sciences
Identification of 2 subunits, named ssSPTa and ssSPTb, for small subunits of Serine Palmitoyl Transferase- these components of the committed and rate-limiting enzyme of sphingolipid synthesis provides important mechanistic insights into the biochemical basis of sphingolipid complexity.
Expression and characterization of novel sphingolipid genes from marine organisms in appropriate yeast mutants and it can provide important insights into the role of these genes in their native hosts.
Uniformed Services University of Health Sciences, Bethesda.
on the project: Elucidation of the Topoisomerase I (TOP1) regulatory pathways and strategies to improve cancer chemotherapy with camptothecins Lys-117 is the major sumoylation site on TOP1, and that the sumoylation of the sites flanking Lys-117 is sufficient to enable TOP1 to be cleared from nucleoli in response to CPT and to prevent the accumulation of TOP1 in nucleoli under basal conditions Identified and characterization the subcellular localization BTBD1 and BTBD2 as novel topoisomerase I-interacting proteins that share 80% amino acid identity.
Also demonstrated the possibility that BTBD2 may modestly restrict HIV-1 infection via colocation with TRIM5 variants in cytoplasmic bodies.
Research Associate Bose Institute - Calcutta, India
Human erythrocyte membrane protein 4.2 is one of the proteins involved in strengthening the cytoskeleton-membrane interactions in the red blood cell.
Deficiency of protein 4.2 is linked with a variety of hereditary haemolytic anaemia.
First demonstrated that palmitoylation serves as a positive modulator of this interaction.
Direct binding of a synthetic peptide, with the sequence corresponding to residues 470-492, to spectrin and the ability of the peptide to inhibit spectrin binding of protein 4.2 confirmed that these residues are crucial in mediating protein 4.2-spectrin interactions.
First demonstrated the critical role of caspase 3 in potentially regulating the life span of the circulating mature human erythrocyte by impairing the activity of the aminophospholipid translocase.
University of Burdwan
Developmental toxicity evaluation of uranyl acetate and methyl- nitrosourea in chick embryos.
Evaluated and established the developmental toxicology and biological effects of N-methyl N-nitrosourea (MNU) in tobacco smoke and nitro stable foods.and uranyl acetate- a potential heavy metal found in the human environment and releating to the possible harmful effects to human exposure.
Genomic DNA and RNA isolation, cDNA preparation, plasmid DNA isolation, DNA transformation, DNA cloning, RACE, gene cloning and nucleotide sequencing, Quickchange method for site directed mutagenesis.
Southern and Northern analysis,.
Cell Culture: Mammalian adherent and suspension cell culture, bacterial culture and culture of cancer cell lines, S.
cerevisiae (yeast cell) culture techniques.
Histological Scanning and Transmission Electron/Immuno-electron Microscopy Techniques: Preparation (paraffin embedding, sectioning etc.) and staining of histological slides.
Experience using Phillips-CM10 transmission electron microscope and photomicrography.
Developmental toxicity testing techniques: Techniques related to developmental toxicity testing in the chick embryo system.
MS : ZoologyPhD Zoology, University of Burdwan University of Burdwan - Burdwan, IndiaZoology
BSc ZoologyUniversity of Burdwan - Burdwan, IndiaTeam Skills Collaborated with other scientists to develop experimental protocols Proven ability to constructively work and communicate with scientists of various disciplines Frequently presented research results to teams of multi-disciplinary scientists for project review Communication, Management and Training skills Strong communication and oral presentation skills, presented talks/posters Managed and trained 4 summer interns Analyzed, evaluated and interpreted data and contributed to scientific articles, managed and maintained orderly functioning of the laboratory. Managed scientific biological projects Assisted in final report preparation for submission of completed research projects/grants. Awards and fellowships National Eligibility Test (NET) examination for lectureship conducted by the Council of Scientific and Industrial Research, Govt. of India (1989) Awarded Graduate Aptitude Test in Engineering (GATE) fellowship by Indian Institute of Technology (1990) in a National level search. Percentile score: 98.08 WORKSHOPS AND TRAINING CBER New Reviewer Training: "The Lifecycle Approach". White Oak, Silver Spring. January 13-16, 2014. Successfully completed training - CBER New reviewer Training: The Lifecycle Approach. Trained in reviewing and evaluating drug applications,FDA laws regulations and guidance documents, overview of the managed review process, IND review process, pre BLA and BLA process, verify compliance with CGMPs , trained in scientific/regulatory issues involving drug products. Proteomics: Principles and Methods. Trac 25, Spring 2005, Bio-Trac. FoundationAdvanced Education in
the Sciences Inc., NIH, Bethesda, Maryland.LIST OF RECENT PUBLICATIONS Moitra PK, Zheng H, Anantharaman V, Banerjee R, Kozakai Y, Gao Y, Lepore T, Krause P, Aravind L, and Kumar S.Genetic and Biological Characterization of Babesia microti Apical Membrane Antigen 1Infection and Immunity, 2015, Oct, 83(10) 3890-91. Khurana B, Zhuang L, Moitra PK, Stantchev TS, Broder CC, Cutler ML and D'Arpa P. HumanTOP1 residues implicated in species specificity of HIV-1 infection are required for interaction with BTBD2, and RNAi of BTBD2 in Old World monkey and human cells increases permissiveness to HIV-1 infection. Virol J. 2010 Nov 20; 7(1):332. Han G, Sita D. Gupta SD, Gable K, Niranjanakumari S, Moitra P, Eichlerc F, Brown RH, Jr., Harmon JM, and Dunn TM. Identification of small subunits of mammalian serine palmitoyltransferase that confer distinct acyl-CoA substrate specificities Proc Natl Acad Sci USA. 2009 May 19;106(20):8186-91. Han G, Gable K, Yan L, Allen MJ, Wilson WH, Moitra P, Harmon JM, Dunn TM.Expression of a novel marine viral single-chain serine palmitoyltransferase andconstruction of yeast and mammalian single-chain chimera. J Biol Chem. 2006 Dec29;281(52):39935-42. Xu L, Yang L, Moitra PK, Hashimoto K, Rallabhandi P, Kaul S, Meroni G Jensen JP,Weissman AM, D'Arpa P. BTBD1 and BTBD2 colocalize to cytoplasmic bodies withRBCC/ tripartite motif protein, TRIM5 delta. Exp Cell Res. 2003 Aug 1; 288 (1): 84-93. Rallabhandi P, Hashimoto K, Mo YY, Beck WT, Moitra PK, and D'Arpa P. Sumoylation of topoisomerase I is involved in its partitioning between nucleoli and nucleoplasm and its clearing from nucleoli in response to camptothecin. J Biol. Chem. 2002 Oct 18; 277(42): 40020-6. Mandal D, Moitra PK, Basu J. Mapping of a spectrin-binding domain of human erythrocyte membrane Protein 4.2. Biochem J. 2002 Jun 15; 364(3): 841-7. Mandal D, Moitra PK, Saha S, Basu J. Caspase 3 regulates phosphatidylserine externalization and phagocytosis of oxidatively stressed erythrocytes. FEBS. Lett. 2002 Feb 27; 513(2-3): 184-8. Moitra PK, Moitra K and Ghosal SK. Developmental toxicity of n-methyl- n- nitrosourea in chick embryos. Environment and Ecology 2001, 19 (2): 334-3399. Paul-Moitra K, Moitra PK,and Ghosal SK. Teratology: A concise overview. Trans. Zool. Soc. East India,2000, 4(1), 35-38. Bhattacharyya R, Das AK, Moitra PK, Pal B, Mandal I, Basu J. Mapping of a palmitoylatable band 3-binding domain of human erythrocyte. Biochem J. 1999 Jun 1; 340 (2): 505-12.
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Companies Worked For:
Uniformed Services University of Health Sciences
University of Burdwan
PhD Zoology, University of Burdwan University of Burdwan
University of Burdwan
Job Titles Held:
Research Associate Post Doctoral Fellow Team leader
MS : Zoology BSc Zoology the Sciences Inc., NIH, Bethesda, Maryland.
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