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Research Assistant Resume Example

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RESEARCH ASSISTANT
Professional Summary
Over 10 years of solid professional and academic experience in Biochemistry and Pharmacy with expertise in cancer biology and enzymology. Adept at design and execution of cellular and biochemical assay, microscopy, data analysis and quantitative analysis. Superior organizational, multitasking, and troubleshooting skills, with exceptional focus and follow-through abilities. Proficiency in managing the researches and studies of different laboratory groups. Excellent communication, interpersonal skills and expertise in presenting research results to colleagues and other professionals.
Core Qualifications
Image J NCBI Program and Software Microsoft Word, Excel, and Power Point GraphPad Prism SAS Adobe Photoshop Adobe Illustrator Latex Editing. Journal Publications Phosphorylation of Cdc42 Effector Protein-4 (CEP4) by Protein Kinase C Promotes Motility of Human Breast Cells. Zhao, X., and Rotenberg, S.A. The Journal of Biological Chemistry 289, 25844
Experience
Research Assistant
January 2009 to Current CUNY-Graduate Center － New York
  • Research Topic: PKCs (Protein Kinase C) signaling pathways in human breast cancer cells.
  • Responsible for developing the molecular methods for isolation and detection the potential PKC substrates in human breast cells.
  • Discovered a novel PKC substrate using in vitro and in vivo kinase assay for the purpose of defining the signaling enzymatic components of isoform-stimulated pathways and providing a predictive marker for human breast cancer.
  • Developed different bio-assays to characterize the functional significance (like cell motility and cell proliferation) of PKC substrates in order to use the substrates as targets of chemotherapeutic agents.
  • Contributed research on evaluating the effects of PKC inhibitor - Dequalinium (anti-tumor agent) and its analogs on PKCs signaling pathways in Human Breast Cancer Cells.
  • Managed grant based and privately funded research related to.
  • Built and maintained collaborations.
  • Supervised and taught technicians master students and undergraduates.
  • Have published 3 peer review paper on this project (2 first author).
Adjunct Lecturer & Graduate Teaching Assistant
January 2009 to January 2014 Queens College － New York
  • Teaching courses: General Chemistry Laboratory * Organic Chemistry Laboratory * Basic Biochemistry Lecture * Advanced Biochemistry Recitation and Laboratory.
  • Experienced in teaching chemistry labs and lectures;.
  • Assisted in re-modify the Laboratory Textbook;.
  • Developed and implanted lesson plans.
Research Assistant
January 2007 to January 2008 HMU-School of Pharmacy, China
Research Topic
August 2006 to December 2006 North China Pharmaceutical Co, Ltd, China
  • Pharmacokinetics study of papaverine hydrochloride using conventional spectrophotometry.
  • Designed a simple and adequate analysis method to determine the papaverine hydrochloride in blood serum;.
  • Assisted in pharmacokinetics study of papaverine hydrochloride in animal model;.
  • Developed a simple and sensitive analytical method for determination of free cortisol human serum with SPE (solid phase extraction)-HPLC (high performance liquid chromatography).
  • Trained in Zymology; learned about its manufacturing lines.
Education
Ph.D : Biochemistry, 1 2014 The Graduate Center, City University of New York － New York, NY Biochemistry Research advisor: Prof. Susan A. Rotenberg. Thesis Topic: Identification and Characterization of Protein Kinase C Substrates in Human Breast Cells.
B.S : Pharmacy, 1 2008 Hebei Medical University Pharmacy
Hebei China Relevant Courses: Pharmacology, Pharmacokinetic/Pharmacodynamic, Pharmaceutical Sciences, Instrumental chemistry, Pharmaceutical Analysis, Pharmacokinetic/Pharmacodynamic, Medicinal Chemistry, Mathematical Statistics, Immunity and Microbiology, Physiology and Human Anatomy.
ASCB international Conference Poster Presentation, San Francisco, 2012. Travel grants from the graduate center of CUNY, New York, 2012. Sigma Xi Poster Presentation, CUNY - Queens College, New York, 2012, 2013. : 1 2013 CUNY - Queens College Hebei Medical University － New York Shijiazhuang, China GPA: Five-year Science Scholarship from Graduate Center of CUNY, New York, 2008-2013. Dean's list of Academic Excellence 25854, 2014. Platinized carbon nanoelectrodes as potentiometric and amperometric SECM probes. Hu et al. Journal of Solid State Electrochemistry 17, 2971-2977, 2013. Analysis of substrates of protein kinase C isoforms in human breast cells by the traceable kinase method. Zhao, X., et al. Biochemistry 51, 7087-97, 2012. SPE-HPLC Determination of free cortisol levels in human serum. Zhao et al. Journal of Hebei University, Natural Science Edition, 4, 2009 Determination of papaverine hydrochloride in human blood and study on its concentration via time curve. Zhao et al. Chinese Journal of Health Laboratory Technology, 20, 18-12, 2008 Honors & Awards The Linakis Award Five-year Science Scholarship from Graduate Center of CUNY, New York, 2008-2013. Dean's list of Academic Excellence
1 2008 Hebei Medical University － Shijiazhuang, China
Accomplishments
  • Recombinant Protein Expression and Purification Separation of Proteins by Chromatography Protein Quantization With Lowry and Bradford Methods Immunodetection and Western Blot Analysis Immunoprecitation (IP) In vitro Kinase Assay.
  • Cell biology:.
  • Primary Cell Culture Construct stable cell lines DNA and RNA Transient and Stable Transfection Flow Cytometry Cell Lysis Techniques Immunocytochemistry RNAi (With siRNA And shRNA), Multiwall Plate-Based Cytotoxicity And Proliferation Assays Migration and Invasion Assay (Wound Healing Assay, Cell Sedimentation Method And Three-D assay).
Certifications
Gene and Recombinant DNA Cloning Genomic PCR RT-PCR Cloning Full Length Genes by PCR Sub-Cloning to Expression Vectors Site Directed Mutagenesis Southern Blot Northern Blot DNA Isolation from Bacteria and Yeast RNA Extraction from MDA-MB-231 Cells.
Interests
UV-Visible Absorption and Fluorescence Spectroscopy Co-Focal and Fluorescence Microscopy HPLC Mass spectrometry Fluorometer and Plate Reading Assays.
Skills
Adobe Photoshop, Basic, C, Chinese, Editing, functional, grants, HPLC, Adobe Illustrator, Image, MB, Excel, 97, Power Point, Microsoft Word, Natural, novel, PCR, Prism, Publications, Research, RT-PCR, San, SAS, Sigma, Statistics, Teaching, author
Additional Information
  • Instrumentation: UV-Visible Absorption and Fluorescence Spectroscopy Co-Focal and Fluorescence Microscopy HPLC Mass spectrometry Fluorometer and Plate Reading Assays.
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Resume Overview

Companies Worked For:

  • CUNY-Graduate Center
  • Queens College
  • HMU-School of Pharmacy, China
  • North China Pharmaceutical Co, Ltd, China

School Attended

  • The Graduate Center, City University of New York
  • Hebei Medical University
  • CUNY - Queens College Hebei Medical University

Job Titles Held:

  • Research Assistant
  • Adjunct Lecturer & Graduate Teaching Assistant
  • Research Topic

Degrees

  • Ph.D : Biochemistry , 1 2014
    B.S : Pharmacy , 1 2008
    Hebei China Relevant Courses: Pharmacology, Pharmacokinetic/Pharmacodynamic, Pharmaceutical Sciences, Instrumental chemistry, Pharmaceutical Analysis, Pharmacokinetic/Pharmacodynamic, Medicinal Chemistry, Mathematical Statistics, Immunity and Microbiology, Physiology and Human Anatomy.
    ASCB international Conference Poster Presentation, San Francisco, 2012. Travel grants from the graduate center of CUNY, New York, 2012. Sigma Xi Poster Presentation, CUNY - Queens College, New York, 2012, 2013. : 1 2013
    1 2008

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