Research Assistant Resume Example University Of Houston, TX

RESEARCH ASSISTANT

Summary

Highly motivated research professional with more than 4 years of academic research experience. Strong molecular biology skills with expertise in mammalian cell culture, CRISPR bases genome editing, gene cloning, recombinant protein expression and purification, bacterial culture, Real-time PCR, chromatin immunoprecipitation, Immunostaining, ELISAs anddata analysis. Proficient in designing, planning and executing scientific experiments independently.

Highlights

Technical Proficiency

Mammalian cell culture of cancer cell lines and cancer stem cells including maintenance, transfection, transduction, single cell cloning and generation of stable cell lines
Protein expression and purification, SDS-PAGE/Western Blot, silver staining
Genome editing using CRISPR-Cas9 system and site directed mutagenesis
Gene cloning including Gateway cloning, Gibson cloning, TOPO cloning
PCR, RT-PCR, qPCR, DNA sequence analysis
Immunoprecipitation, Co-Immunoprecipitation
Immunofluorescence and Immunoenzymological staining
Gel electrophoresis
DNA, RNA and plasmid isolation and purification
Southern blotting, Northern blotting
Chromatin immunoprecipitation (ChIP), ELISA
Flow Cytometry, fluorescent microscopy
Knowledge of sequence alignment tools like BLAST and FASTA

Computer Proficiency

MS-Office (Word, Excel, Power Point), OSX

Professional proficiency

Excellent communication skills strengthened through teaching undergraduate biology lab
Organizational and team management skills by organizing intro biology laboratory class for around 1000 students

Data Analysis

R
Python

Experience

Research Assistant02/2013 to 02/2017
University of Houston, TX

Assessing the oncogenic potential of MESP1 gene

Knocked out MESP1, a key regulator of cardiovascular progenitors in vertebrates, by either deleting a large DNA fragment or deletion or addition of a few nucleotides via CRISPR-Cas9 system.
Isolated single cell cones with homozygous, heterozygous or hypomorphic mutations.
Assessed the transfection efficiency of various shRNAs against Mesp1 by cloning MESP1 into mammalian expression vectors and transfecting the vectors into cell lines for testing.
Generated Mesp1 knockdown stable cell lines using lentiviral mediated transduction with shRNAs and CRISPR-Cas9 system.
Performed various tumorigenic assays.
Proliferation and viability assays using cell counting and calorimetric quantitation.
Anchorage independence, colony formation, invasion and migration assays.
Metabolic, cytotoxicity and apoptotic assays.
Isolated and cultivated cancer stem cells (CSC) for oncogenic testing by.
CCS markers like CD133, CD44, Aldehyde dehydrogenase activity.
Chronic exposure to anticancer drugs, serum free culture to generate oncospheres.
Explored Mesp1 targets by chromatin immunoprecipitation and real time PCR

Project: Development of gold nanoparticle platform for the delivery of functional microRNAs into cancer cells.

10/2012 to 10/2013

Cysteamine functionalized gold nanoparticles conjugated to chemically unmodified microRNAs (miR1-AuNP10-S-PEG0.5) were used to assess the delivery efficiency of microRNAs.
P53-mutant and p53-wild type ovarian cancer cell lines (OVCAR8 and HEYA8) were used for transfection at different concentrations.
Taqman miRNA assays were used to perform qRT-PCR to evaluate the transfection efficiency of miR-AuNP-S-PEG mediated treatment.

Teaching Assistant10/2012 to 04/2016

University of Houston.
Introduction to Biology Laboratory, Undergraduate course at University of Houston- Instructed a class of 48 undergraduate students, assisted in performing their experiments and grading their reports and final exams.

Research Intern01/2010 to 01/2011
University of DelhiNew Delhi, India

Synthesis of Thiazolo-Pyrimidine derivatives and evaluation of their biological properties.
Combined pyrimidine and thiazolium pharmacophore into a single entity possessing A2A receptor antagonistic activity thereby acting as potent anti-parkinsonian agents.
Synthesized 3-R-7-imino-6-(N-piprazine ethylamine)-2H,3H,6H,7H- [1,3]thiazolo[4,5-d]pyrimidine-2-thione compounds with varying R substituents.
Derivatives with R being ethyl and propyl groups were the most active compounds with optimal drug-like properties.
COURSES TAKEN Molecular genetics, Cell Biology, Nucleic Acids, Stem cell biochemistry, Eukaryotic gene regulation.

Education

M.S: Cell and Molecular Biology Biology and BiochemistryMay 2017University of HoustonCell and Molecular Biology Biology and Biochemistry 3.668

M.Sc. (Master of Science): Biomedical Sciences2011University of DelhiIndiaBiomedical Sciences

B.Sc. (Bachelor of Science): Life Sciences2008University of DelhiIndiaLife Sciences

Interests

Currently on F1. Will receive OPT.

Skills

biochemistry, Biology, cancer, cell culture, Excellent communication, Data Analysis, delivery, Derivatives, DNA, editing, ELISA, experiments, functional, Gateway, genetics, Excel, MS-Office, Power Point, Word, migration, Northern blotting, organizing, Organizational, PAGE, PCR, Python, real time, RT-PCR, Southern blotting, teaching, team management, type

Additional Information

PERSONAL INFORMATION Currently on F1. Will receive OPT.

Resume Overview

Companies Worked For:

University of Houston, TX
University of Delhi

School Attended

University of Houston
University of Delhi

Job Titles Held:

Research Assistant
Teaching Assistant
Research Intern

Degrees

M.S : Cell and Molecular Biology Biology and Biochemistry May 2017
M.Sc. (Master of Science) : Biomedical Sciences 2011
B.Sc. (Bachelor of Science) : Life Sciences 2008

Research Assistant Resume Example

Resume Score: 90%

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