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Postdoctoral Associate Resume Example

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POSTDOCTORAL ASSOCIATE
Professional Summary
Highly skilled molecular biology and cell biology with 12 years in clinical research and laboratory testing. Research Biologist practiced in cell-based assay development, molecular biology and genetics, as well as drafting corresponding technical and research documents. Biologist with 6 years in leading large teams of scientists in advanced research projects. Extremely knowledgeable in overall laboratory function and maintenance.
Skills
  • RNA isolation knowledge
  • Proficient in PCR
  • In-vivo transcription
  • Skilled in genotyping
  • Published researcher
  • Protein isolation
  • Gene expression
  • Cytoplasmic protein extraction
  • ELISA assay
  • UV and Vis Spectrometry
  • Immunohistochemistry assay knowledge
  • Published author
  • American Institute of Biological Sciences
Languages
English- Fluent (reading/writing/speaking/understanding) I am fluent in English and
Work History
Postdoctoral Associate06/2015 to 06/2016
University of Pittsburgh, School of Medicine. Vascular Medicine Institute, Pulmonary, Allergy and Critical Care Medicine – Pittsburgh, USA, PA
  • Molecular analysis of signaling mechanisms that regulating cellular energy metabolism, proliferation and survival in pathogenesis of pulmonary arterial hypertension (PAH)".

Research Associate09/2014 to 05/2015
The Scripps Research Institute, La Jolla – San Diego, USA, California
  • Project Title

Research Associate07/2013 to 11/2013
ICDDRB – Dhaka, Bangladesh
  • Tuberculosis Laboratory, Laboratory Sciences Division, ICDDR, B in Bangladesh.
  • Conducted basic and applied research on molecular biology.
  • Collected and analyzed biological data about relationships between organisms and their environment.

PhD student11/2009 to 04/2013
University of Paris – FRance
  • PhD student, INRA umr1319 MICALIS, Microbiology and Molecular Genetics Lab, CBAI, INRA, Paris, France.
  • PhD defends at Universite Paris-Sud, Paris, France.
  • Thesis title "Physiological roles of Eukaryotic Hanks type Ser/Thr kinases in transition to stationary phase in Bacillus subtilis".
  • Thesis summary

Research technician07/2009 to 10/2009
CBAI, INRA – Paris, France

  • Master research student (M.Sc thesis), AgroParisTech in Microbiology and Molecular Genetics Lab, INRA, Paris, France.
  • Thesis title "Screening for physiological substrates of Ser/Thr protein kinases in Bacillus subtilis".

Master research student02/2007 to 07/2008
Molecular Genetics Department

active01/2006 to 01/2007
Linkoping University
  • On leave up to 2009 Scientific Officer, Columbia University, USA and Arsenic and Health Research Office in Bangladesh.
  • Project title

Research Officer01/2005 to 01/2006
ICDDRB – Bangladesh
  • Environmental Microbiology Laboratory, under Laboratory Sciences Division, ICDDR, B in Bangladesh.
  • The project title "Vibrio choleraeO1 EPS structure, regulation and function" funded by Stanford University, USA.
  • Another collaborative study "to investigate whether V.
  • cholerae O1 can form biofilms in the aquatic environment to survive during the inter-epidemic periods" funded by Stanford University, California, USA.

Senior Research Assistant01/2004 to 01/2005
ICDDRB – Bangladesh
  • Environmental Microbiology Laboratory, Laboratory Sciences Division, ICDDR,B in Bangladesh.
  • Project title

Master student01/2003 to 01/2004
ICDDRB – Bangladesh
  • Masters research student, Environmental Microbiology Laboratory, Laboratory Science Division, ICDDR, B to complete the fulfillment of Master of Science in Nutrition to Dhaka University, Bangladesh.
  • Technical expertise In mammalian cells Experimental design of hypoxia induced rat and mouse model for therapeutic intervention of pulmonary vascular remodelling in PAH.
  • Cell culture, cloning and transfection to mammalian cell line ( vascular smooth muscle cells of human, mouse and rat), Mammalian cell lysis, western blotting, Immunohistochemistry, ELISA, PCR, RT-PCR, Quantitative PCR.
  • CRISPER mice RFLP genotyping and sequencing analysis.
  • Bioinformatic analysis for protein-protein interaction.
  • Hemodynamic measurements analysis.
  • Extraction of genomic DNA, RNA, mononuclear cells & granulocytes from whole blood from Arsenic induced cancer patients.
  • In bacteria Molecular biology techniques such as gene cloning and truncation, inactivation, deletion, mutation, transformation, gene expression and regulation analysis Ligation independent cloning, live cell array, beta gal assay.
  • Bioinformatics, RNA extraction and transcriptomic analysis In vitro and in vivo protein phosphorylation and dephosphorylation assay Western blotting, PCR, RT-PCR, Quantitative PCR.
  • DNA Recombination assay, Electrophoretic mobility shift assay Expression, purification and analysis of recombinant proteins Gel filtration assay, Fluorescence antibody technique using fluorescence microscopy Antibiotic susceptibility assay, various enzymatic assay, fluorescence-localization approaches Sporulation, competence, protease production, biofilm, swarming assays etc Classical bacteriology techniques for isolation and detection of pathogens from water, food, fish, environmental samples Supervising experience.

supervise students01/2010 to 01/2012
University of Paris – France
  • Teaching undergraduate students and supervised M.Sc thesis students during my PhD thesis period.

  • Teaching and helping experimental works of undergraduate, M.Sc and Ph.D students of my research group during my Postdoctoral training period.
  • Publications in peer-reviewed journals Tatiana V.
  • Kudryashova, Dmitry A.
  • Goncharov, Andressa Pena, Neil Kelly, Rebecca Vanderpool, Jeff Baust, Ahasanul Kobir, William Shufesky, Adrian E.
  • Morelli, Jing Zhao, Kaori Ihida-Stansbury, Horace DeLisser, Rubin M.
  • Tuder, Steven M.
  • Kawut, Herman H.W.
  • Silljé, Steven Shapiro, Yutong Zhao, Elena A.
  • Goncharova.
  • HIPPO-integrin linked kinase crosstalk regulates pulmonary vascular smooth muscle cell proliferation and survival in pulmonary hypertension", Am J Respir Crit Care Med.
  • 2016 Apr 27.
  • Epub ahead of print] Ahasanul Kobir, Sandrine Poncet, Vladimir Bidnenko, Carsten Jers, Samira Zouhir, Sylvie Nessler, Marie-Françoise Noirot-Gros and Ivan Mijakovic "Phosphorylation of the Global gene regulator AbrB from Bacillus subtilis influences its DNA binding." Molecular Microbiology.
  • 2014 Jun;92(5):1129-41.
  • doi: 10.1111/mmi.12617.
  • Epub 2014 Apr 29.
  • Vaishnavi Ravikumar, Lei Shi, Karsten Krug, Abderahmane Derouiche, CarstenJers, Charlotte Cousin, Ahasanul Kobir, Ivan Mijakovic, Boris Macek.
  • Quantitative phosphoproteome analysis of Bacillus subtilis reveals novel substrates of the kinase PrkC and phosphatase PrpC" Molecular Cell Proteomics, January 5.
  • 2014.
  • Manuscript M113.035949 Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Ventroux, Céline Henry, Alain Trubuil, Marie-Françoise Noirot-Gros, Ivan Mijakovic.
  • Bacillus subtilis serine/threonine kinase YabT phosphorylates the recombinase RecA" Molecular Microbiology.
  • 2013 Jun; 88(5):921-35.
  • Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic.
  • Bacillus subtilis Two-Component system sensory kinase DegS is regulated by Serine phosphorylation in its input domain".
  • PLoS One.
  • 2011, 6 (2):e 14653 21304896 Ahasanul Kobir, Lei Shi, Ana Boskovic, Christophe Grangeasse, Damjan Franjevic, Ivan Mijakovic.
  • Protein phosphorylation in bacterial signal transduction." Biochim Biophys Acta.
  • 2011, 1810: 989-994 Lei Shi, Ahasanul Kobir, Carsten Jers and Ivan Mijakovic.
  • Bacterial Protein-Tyrosine Kinases."Current Proteomics, 2010, 7: 188-194 Manuscript Submitted 8) Maryam Sharifi-Sanjani, Dmitry Goncharov, Jeffrey Baust, Baojun Chang, Ahasanul Kobir, Andressa Pena, Mark Ross, Claudette St.
  • Croix, Jeffrey.
  • Isenberg, Charles F.
  • McTiernan, Christine S.
  • Moravec, and Elena A.
  • Goncharova.
  • Yes-associated Protein (Yap) is Up-Regulated in Heart Failure and Promotes Cardiac Fibroblast Proliferation" American Journal of Physiology-Cell Physiology.
  • Manuscripts under preparation 9) * Ahasanul Kobir, Akiko Goda, Antonia Delgado-Montero, Bhupendar Tayal, Rebecca Vanderpool, Jeffrey Baust, Andressa Pena, Dmitry Goncharov, John Gorcsan, Elena Goncharova.
  • Pharmacological inhibition of mTOR kinase activity reverses sugen/hypoxia-induced pulmonary hypertension, right ventricular hypertrophy, and improves heart functional parameters in rats" 2016 Conferences Oral presentations Ahasanul Kobir " Canonical Pathway of MST1/2(HIPPO signaling) in Pulmonary Arterial Hypertension".
Pulmonary, Allergy and Critical Care Medicine, Vascular Medicine Institute, University of Pittsburgh Medical Center – Pittsburgh, USA, PA
  • Ahasanul Kobir "Pharmacological inhibition of mTOR kinase activity reverses sugen/hypoxia-induced pulmonary hypertension, right ventricular hypertrophy, and improves heart functional parameters in rats".
American Heart Association Research Day, University of Pittsburgh – PA
  • Ahasanul Kobir "Investigating the transcriptional and posttranscriptional regulation of essential autolysin function in Gram-positives and how these different levels of control are intertwined" Molecularbiology and Experimental Medicine, The Scripps Research Institute, La Jolla, CA.
  • USA, 2015 Ahasanul Kobir "Bacillus subtilis serine/threonine kinase YabT phosphorylates the recombinase RecA" Centro Nacional de Biotecnologia (CSIC), Departamento de Biotecnologia Microbiana, Madrid, SPAIN.
  • 2014 Ahasanul Kobir "Phosphorylation of the Global gene regulator AbrB from Bacillus subtilis influences its DNA binding." Dept.
  • of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.
  • 2013 Ahasanul Kobir.
  • Sandrine Poncet.
  • Vladimir Bidnenko.
  • Carsten Jers.
  • Samira Zouhir, Sylvie Nessler.
  • Marie-Françoise Noirot-Gros and Ivan Mijakovic."Physiological roles of Eukaryotic Hanks type Ser/Thr kinases in transition to stationary phase in Bacillus subtilis" Journée de doctorants GGC, University of Paris Sud, France, February 2012.
  • Ahasanul Kobir.
  • Sandrine Poncet.
  • Vladimir Bidnenko.
  • Carsten Jers.
  • Samira Zouhir, Sylvie Nessler.
  • Marie-Françoise Noirot-Gros and Ivan Mijakovic.
  • Physiological roles of Eukaryotic Hanks type Ser/Thr kinases in transition to stationary phase in Bacillus subtilis" Journée de Doc'Micalis, Jouy-en Josas, Paris, France, Octobe 2011 Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Rault-Ventroux, Alain Trubuil, Marie-Françoise Noirot-Gros, and Ivan Mijakovic "Phosphorylated bacterial recombinase checks chromosome integrity during spore formation" BACEL meeting, Gottingen, Germany, March 2011 Ahasanul Kobir & Ivan Mijakovic"Screening for physiological substrates of Ser/Thr protein kinases in Bacillus subtilis".
DPT MICA JOURNEES DES DOCTORANTS – Paris, France
  • Ahasanul Kobir, CarstenJers, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic.
  • Bacillus subtilis Two-Component System sensory kinase DegS is regulated by serine phosphorylation in its input domain".
  • 35th FEBS Congress, June 2010, Gothenburg, Sweden.
  • Ahasanul Kobir & Ivan Mijakovic "Screening for physiological substrates of Ser/Thr protein kinases in Bacillus subtilis".
Paris, France
  • Poster presentations.
  • Ahasanul Kobir, Akiko Goda, Antonia Delgado-Montero, Bhupendar Tayal, Rebecca Vanderpool, Jeffrey Baust, Andressa Pena, Dmitry Goncharov, John Gorcsan, Elena Goncharova.
  • Pharmacological inhibition of mTOR kinase activity reverses sugen/hypoxia-induced pulmonary hypertension, right ventricular hypertrophy, and improves heart functional parameters in rats".
  • ATS 2016 international conference, San Francisco, May 2016.
  • Ahasanul Kobir, Akiko Goda, Antonia Delgado-Montero, Bhupendar Tayal, Rebecca Vanderpool, Jeffrey Baust, Andressa Pena, Dmitry Goncharov, John Gorcsan, Elena Goncharova.
  • Pharmacological inhibition of mTOR kinase activity reverses sugen/hypoxia-induced pulmonary hypertension, right ventricular hypertrophy, and improves heart functional parameters in rats".
American Heart Association Research Day, University of Pittsburgh – PA
  • Ahasanul Kobir, Vladimir Bidnenkoand Ivan Mijakovic"Phosphorylation of the Global gene regulator AbrB from Bacillus subtilis influences its DNA binding".
  • 6th International Conference for Gram-positive Microorganisms, June 2011, Tuscany, Italy Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic.
  • Bacillus subtilis Two-Component System sensory kinase DegS is regulated by serine phosphorylation in its input domain." 35th FEBS Congress, June 2010, Gothenburg, Sweden Awards and achievements.
San Francisco, USA
  • Master programme scholarship from Swedish government.
PhD supervisor01/1998 to 01/2001
Ivan Mijakovic
Director Systems and Synthetic BiologyChalmers University of Technology
  • Kemivägen 10 SE-412 96 Göteborg SWEDEN E-mail: ivan.mijakovic@chalmers.se www.sysbio.se Professor with Special Responsibilities Novo Nordisk Foundation Center for Bio-sustainability Technical University of Denmark Kogle Allé 6 DK-2970 Hørsholm DENMARK www.biosustain.dtu.dk Dr.
  • Josef Deutscher ( Tutor of my doctoral school).
Education
Ph.D.: Molecular GeneticsNov2012Université Paris-Sud- ParisMolecular Genetics
Master of Science: Food Biotechnology2009AgroParisTech- ParisFood Biotechnology
Master of Science: Molecular Genetics and Physiology2008Linkoping University- SwedenMolecular Genetics and Physiology
Master of Science: Nutrition2004University of Dhaka- BangladeshNutrition
Bachelor of Science: Biochemistry2001Aligarh University- IndiaBiochemistry
Additional Information
  • Visa status: Pending green card, EAD card valid until August 2018
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Resume Overview

Companies Worked For:

  • University of Pittsburgh, School of Medicine. Vascular Medicine Institute, Pulmonary, Allergy and Critical Care Medicine
  • The Scripps Research Institute, La Jolla
  • ICDDRB
  • University of Paris
  • CBAI, INRA
  • Molecular Genetics Department
  • Linkoping University
  • Pulmonary, Allergy and Critical Care Medicine, Vascular Medicine Institute, University of Pittsburgh Medical Center
  • American Heart Association Research Day, University of Pittsburgh
  • DPT MICA JOURNEES DES DOCTORANTS
  • Ivan Mijakovic
  • Chalmers University of Technology

School Attended

  • Université Paris-Sud
  • AgroParisTech
  • Linkoping University
  • University of Dhaka
  • Aligarh University

Job Titles Held:

  • Postdoctoral Associate
  • Research Associate
  • PhD student
  • Research technician
  • Master research student
  • active
  • Research Officer
  • Senior Research Assistant
  • Master student
  • supervise students
  • PhD supervisor
  • Director Systems and Synthetic Biology

Degrees

  • Ph.D. : Molecular Genetics Nov2012
    Master of Science : Food Biotechnology 2009
    Master of Science : Molecular Genetics and Physiology 2008
    Master of Science : Nutrition 2004
    Bachelor of Science : Biochemistry 2001

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