- Molecular Biology: mRNA and protein extraction, PCR, RT-PCR, Quantitative-PCR, Sequencing, Cloning, Mutagenesis, Bacterial transformation, Plasmid production and purification, Adenovirus production and purification, Western blot. Production and purification of recombinants proteins, G-lisa, co-immunoprecipitation, BIAcore 3000.
- Cellular Biology: Cell culture (HeLa, HaCaT, MDCK, L-cells, DLD1R,
HL1), Stable transfection. Rat and mice neonatal cardiomyocytes
isolation.
- Animal Biology: Mouse breeding and colony management, adult and neonatal (subcutaneous and peritoneal) injection, dissection, Heart isolation and canulation. Zebrafish breeding, heart dissection.
- Histology: Heart frozen and paraffin section immunohistofluorescence.
| - Microscopy: Usage of Olympus IX50 microscope and 3D deconvolution. Zeiss LSM 510 META Confocal Microscope System
- Computer Literacy: Microsoft Office, Sigma Plot, Graphpad Prism, Image J, Metamorph, Photoshop,
- Other: Project management and writing of research publications.
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