Molecular Biologist with over 7 years of research experience; productive as an individual and team player resulting in 12 peer reviewed journal publications; strong background and expertise in molecular and biochemical techniques; experienced in technical and grant proposal writing.
Molecular techniques: PCR, qRT-PCR, Primer design, Chromatin Immunoprecipitation (ChIP), Co-Immunoprecipitation, Immunohistochemistry, FISH, Restriction digestion, DNA ligation, Radiolabeling of probes, Northern Blot, Primer extension analysis, Immunohistochemistry, Epitope tagging of proteins, Gene deletion, Site directed mutagenesis
Protein biochemistry: Vector design, cDNA library construction, Cloning, expression and purification of proteins in bacteria and insect cells, SDS-PAGE, Western blot
Bioinformatics: BLAST, Multiple Sequence Alignment, Online databases Microsoft Office Suite (Word, PowerPoint, Excel), Basics to C, C++, Adobe Illustrator, Adobe Photoshop
Imaging: Confocal and Fluorescent microscopy
Post-doctoral Researcher/Specialist September 2013 to CurrentUniversity of California － Irvine, CA
Instrumental in design and development of biochemical and mass spectrometric tools for the identification of metabolite-protein interactions, using mammalian cullin RING ligases as metabolite sensors.
Laboratory management skills like ordering lab supplies and maintaining safety standards.
Graduate Research Assistant August 2007 to August 2013Southern Illinois University － Carbondale, IL
Discovered a role of new class of ubiquitin ligase complex in mRNA export regulation that was instrumental in obtaining $50K grant and an invited written review from a prestigious journal.
Developed novel protocols for transcriptional initiation and expression analysis of antisense mRNA using RT-PCR and immunoprecipitation methods.
Spearheaded four projects to completion within the given timeframe and budget to generate interesting observations , contributing to first author publications.
August 2008 to May 2013Southern Illinois University － Carbondale, IL
Designed and implemented rotation projects for 9 first year graduate students in my lab.
Trained students in laboratory techniques and motivated them to become independent researchers.
Research Intern June 2004 to August 2004Central Leprosy Training and Research Institute － Chengalpattu, India
Developed a PCR-based method to diagnose Mycobacterium tuberculosis infection in clinical samples.
Work presented and selected as best presentation in a national symposium.
Bachelor of Technology : Industrial Biotechnology, May '07Anna UniversityIndiaGPA: Awarded National Merit certificate for excellence in Secondary School examination 2001
School Topper in 10th grade board examinationsIndustrial Biotechnology
Ph. D : Biochemistry and Molecular Biology, August '13Southern Illinois University, School of Medicine － Carbondale, ILBiochemistry and Molecular Biology
Department of Biological chemistry
Department of Biochemistry and Molecular Biology
Shukla, A.*, Durairaj, G*, Schneider, J., Duan, Z., Shadle, T., and Bhaumik, S. R. (2009). Stimulation of mRNA export by an F-box protein, Mdm30p, in vivo. Journal of Molecular Biology, 389: 238-247. Durairaj, G., Garg, P., and Bhaumik, S. R. (2009). Nuclear export of mRNA and its regulation by ubiquitylation. RNA biology, 6:531-535. (Invited review) Malik, S.*, Chaurasia, P.*, Lahudkar, S.*,Durairaj, G*, Shukla, A., Bhaumik, S. R. (2010). Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II dependent manner in vivo. Nucleic Acids Research, 38, 1461-1477. Durairaj, G*, Chaurasia, P.*, Lahudkar, S.*, Malik, S.*, Shukla, A., Bhaumik, S. R. (2010) Regulation of chromatin assembly/disassembly by Rtt109p, a histone H3 Lys56-specific acetyltransferase, in vivo. Journal of Biological Chemistry, 285, 30472-9. Lahudkar, S.*, Shukla, A. *, Bajwa, P. *,Durairaj, G.*, Stanojevic, N., Bhaumik, S. R. (2011) The mRNA cap-binding complex stimulates the formation of pre-initiation complex at the promoter via its interaction with Mot1p in vivo. Nucleic Acids Research, 39, 2188-209 Shukla, A., Lahudkar, S., Durairaj, G., and Bhaumik, S.R. (2012). Sgf29p facilitates the recruitment of TATA box binding protein but does not alter SAGA's global structural integrity in vivo. Biochemistry, 51:706-714 Sen, R., Lahudkar, S., Durairaj, G., and Bhaumik, S.R. (2013). Functional analysis of Bre1p, an E3 ligase for histone H2B ubiquitylation, in regulation of RNA polymerase II association with active genes and transcription in vivo. Journal of Biological Chemistry, 288: 9619-9633. Malik, S.*, Durairaj, G.* and Bhaumik, S.R. (2013). Mechanisms of antisense transcription initiation from the 3'-end of the GAL10 coding sequence in vivo. Molecular and Cellular Biology, 33: 3549-3567. Lahudkar, S., Durairaj G., Uprety B., and Bhaumik, S.R. (2014). A Novel Role for Cet1p mRNA 5'-Triphosphatase in Promoter Proximal Accumulation of RNA Polymerase II in Saccharomyces cerevisiase. Genetics, 196: 161-176. Durairaj, G., Lahudkar, S., and Bhaumik, S.R. (2014). A new regulatory pathway of mRNA export by an F-box protein, Mdm30. RNA, 20: 133-142 Durairaj, G., Sen, R., Uprety, B., Shukla, A., Bhaumik, S.R.( 2014).Sus1p Facilitates Pre-Initiation Complex Formation at the SAGA-Regulated Genes Independently of Histone H2B De-Ubiquitylation. J Mol Biol.426:2928-2941 Durairaj, G., and Kaiser, P. (2014). The 26S Proteasome and Initiation of Gene Transcription. Biomolecules, 4: 827-847. Equal contribution)
Adobe Photoshop, budget, C, C++, databases, Adobe Illustrator, Imaging, management skills, Excel, Microsoft Office Suite, PowerPoint, Word, novel, PAGE, PCR, protocols, publications, RT-PCR, safety, symposium, author, written
Awards and achievements:
Awarded Indian Oil Youth Scholarship to pursue undergraduate studies 2003-2007
Awarded Merit scholarship from State Government of Tamilnadu for undergraduate studies 2003-2007
Best paper presentation award in a symposium organized by Annamalai university, India 2005