Detail-oriented, newly certified Medical Technologist/Medical Laboratory Technician with extensive knowledge of genetics, microbiology, immunology, blood bank and hematology. Familiar with preparing cultures, staining specimens, analyzing and reporting data and calibrating equipment. Extensive research experience with excellent troubleshooting skills.
Medical Technologist- generalist (AAB)
Medical Laboratory Technician (ASCP)
Microbiology Medical Technologist for a busy reference lab. Perform moderate and high complexity testing on clinical specimens for diagnostic purposes. Responsible for culture set-up, kit testing (including but not limited to HsPA EIA, STAT EHEC, Affirm, lateral flow Crypto/Giardia, Illumigene C. diff, Illumigene Group A strep and Illumigene Group B strep). Assisted the technical specialist with validation study for bringing on Cephiad MRSA and TB testing.Responsible for daily and monthly maintenance of various lab equipment such as centrifuges, Isoplator, Vitek, Microscan and BacT machines. Familiar with cell culture techniques for the purposes of virology testing.
Responsible for culture set-up and moderate complexity kit testing in a very busy reference microbiology lab. Experience included Vitek set-up, Mycobacteria and fungal culture set-up and specimen preparation for Gram stain and Acid Fast staining.
Primary research focused on the effects of genetics in microbial colonization and the role of miocrobiota in normal gut development and disease using zebrafish and stickelback as model organisms. Developed protocols and assays to assist the University of Oregon zebrafish facility with detection of Mycobacterium marinum from environmental samples.
Pioneered protocols for developing and raising juvenile germ-free zebrafish and stickleback. Contributed to the development of the Gnotobiology Core at the University of Oregon. Investigated the composition of Zebrafish and stickleback microbiota using fluorescence in-situ hybridization, quantitative real-time PCR, DNA sequencing, terminal-restriction fragment length polymorphism and standard culturing and enumeration techniques. Assisted in Zebrafish husbandry and maintenance of stocks. Maintained lab supplies and equipment. Mentored undergraduate and graduate students. Trained new lab members in lab safety and standard protocols. Provided technical support to collaborators outside of the University including labs in other countries.
Training included clinical rotations through Hematology, urinalysis, coagulation, chemistry, blood bank, microbiology, special chemistry and molecular biology.
Summa cum laude
Milligan-Myhre, K., Small, C.M., Mittge, E.K., Agarwal, M., Cresko, W.A., Guillemin, K. Innate immune responses to gut microbiota differ between threespine stickleback fish populations. 2015. Submitted for publication. Disease Models and Mechanisms.
Roeselers G*, Mittge EK*, Stephens WZ, Parichy DM, Cavanaugh CM, Guillemin K, Rawls JF. Evidence for a core gut microbiota in the zebrafish. ISME J. 2011Oct;5(10):1595-608. doi: 10.1038/ismej.2011.38. Epub 2011 Apr 7.
Cheesman SE, Neal JT, Mittge E, Seredick BM, Guillemin K. Epithelial cell
proliferation in the developing zebrafish intestine is regulated by the Wnt
pathway and microbial signaling via Myd88. Proc Natl Acad Sci U S A. 2011 Mar15;108 Suppl 1:4570-7. Epub 2010 Oct 4.
Bates JM, Akerlund J, Mittge E, Guillemin K. Intestinal alkaline phosphatase
detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota. Cell Host Microbe. 2007 Dec 13;2(6):371-82.
Bates JM*, Mittge E*, Kuhlman J, Baden KN, Cheesman SE, Guillemin K. Distinct signals from the microbiota promote different aspects of zebrafish gut
differentiation. Dev Biol. 2006 Sep 15;297(2):374-86. Epub 2006 May 11.
*Indicates shared first author status
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