To help contribute to research interests in biological and chemical systems.
Enzyme Linked Immunosorbent assay (ELISA) X-ray crystallography - real time, quantitative PCR Western, Southern, Northern blot - plasmid transfection, recombinant DNA methodology High pressure liquid chromatography (HPLC) Mass Spectrometry, NMR - Electro-encephalography Gas Chromatography/UV Spectroscopy
Publications, Presentations, and Awards Publications Pending: (January 2013) Novel Methods for Assessing Pain and Anxiety in Neuropathic Models of Post-Traumatic Stress Disorder - Stanford University School of Medicine. Tam, F.Y.
Complement (C') Activation through A*-C1q binding in Classical Component Pathway." - A presentation for MB & B students and staff,UCI's McGaugh Hall, Irvine, CA. August 28, 2003.
2nd Place, UC Irvine Biomedical Research Student Seminar.
Complement (C') Activation through A*-C1q binding in Classical Component Pathway" August, 2003.
B.S: Cell and Molecular Biology
University of California, Irvine EvergrA.S: Molecular Biology & Biochemistry Applied Mathema
Stanford University School of MedicinePalo Alto, CALife Science Research Assistant
Main projects: Amyloid Pre-cursor protein (APP) in Alzheimer's disease (AD), Posttraumatic stress disorder (PTSD) and post-operative pain, Beta-adrenergic receptor pathways.
Utilize Stanford Genome Technology Center - AMOS (Automated Multi-plex Oligonucleotide Synthesizer) machines for high-throughput synthesis of T41B, NPas4, other genes involved in neurological abnormalities.
Extensive use of real time and quantitative PCR for bio-chemical pathology of AD and neuropathic PTSD.
Genotyping of transgenic mouse lines, APP and NPas4 (DNA/RNA extraction, PCR, Gel Electrophoresis) Molecular studies in cognitive function Experimental primary neuron cell culture.
Hughes Research Laboratories, LLCMalibu, CAResearch Scientist
National Science Foundation research - Optical Imaging/computer vision.
Used "Bio Semi" Electro-encephalography (EEG) system to study object recognition and tracking.
Developed algorithms around statistical parametric mapping.
fMRI - radio-labeling isotopes development.
Jonsson Comprehensive Cancer Center, UCLALos Angeles, CAResearch Associate
Gene expression and regulation of NSC markers (Nestin, SOX2, MELK) examined by construction of specific siRNA oligonucleotide sequence (AME Biosciences, 'MerMade 4' - equipped with Trityl monitoring).
Analyzed telomerase activity in brain tumor stem cells with Western blot utilizing hTERT antibody.
cDNA microarray analysis of neural stem cells to determine molecular mechanisms behind temporal evolution.
UCLALos Angeles, CAGraduate Research Fellow
Determine enzymatic molecular structure of Maternally derived Embryonic Leucine-Kinase (MELK) protein via Raman spectroscopy.
Peptide synthesis of GFAP peptide using cross-linked polystyrene resin support (aminomethyl resin).
Institute for Pediatric Regenerative Medicine, USacramento, CASenior Research Associate, III
Project on Multiple Sclerosis via Experimental Auto-Immune Encephalomyelitis (EAE) model.
Immune response to Myelin Oligodendrocyte Glycoprotein (MOG) in transgenic NF-** mice.
Tandem Mass spectrometry for in vitro phosphorylation of several cell markers including Poly ADP ribose polymerase (PARP), interleukins (IL-1 and IL-17), and cytokines.
real-time and quantitative PCR analysis of those genes (day 1 - day 44 immune response) Primer design and synthesis ELISA/Western Blot for PARP, IL-1 and IL-17, and cytokines.
Isolation and maintenance of lymphocyte cell culture lines (mouse spleen).
Cytokine, interleukins isolation from tissue (spinal cord, brain, lymph nodes), Real time and quantitative PCR.
Methods include: confocal and electron microscopy, fixation/fluorescent antibody staining, flow cytometry.
National Genetics InstituteLos Angeles, CALaboratory Technologist, II
Preparation of samples for analysis of blood-borne pathogens - HIV, Hepatitis A, B, C, etc.
Gene sequence analysis of BSL-2 antigens HIV, Hepatitis, Parvo Virus, and Tuberculosis.
single base pairs HPLC-purified Solid Support/Phase oligonucleotide synthesis for structural modeling of novel mechanisms inhibiting-HIV replication Phosphoramidite construction method NMR characterization of 35mer containing 4-thio-deoxyuridilate (s4dU)35 DNA extraction/Cell lysis via Guanidine iso-thiocyanate, reverse transcription of DNA examination with Southern Blot.
Cal-State Los AngelesLos Angeles, CAResearch
Monitored the kinetic behavioral response to robot assisted hind-limb movement with an original algorithm that reduced high frequency variations in movement, spinal cord injury.
Verified presence of Brain Derived Neurotrophic Factor (BDNF) in motor neuron and tissue regeneration through in situ hybridization.
University of CaliforniaIrvine, CA
Complement immune response system activation in Alzheimer's disease related dementia.
Interaction of cellular signaling molecules determined through hemolytic volumetric analysis (C1q, C3, and C4d) and tissue culture.
Enzyme linked immunosorbent assay (ELISA) validation: compare/quantify C1q levels with A*, IFN, and A* + IFN.
Developed protocol to ascertain iNOS concentration in neurofibrillary plaques.
University of CaliforniaIrvine, CA
Iso-aspartate radial group derived from asparagine side chain via HPLC, ion exchange, affinity chromatography.
Trace labels through Paraneoplastic Neurological Syndrome pathogenesis.
3H - labeled methylase donated by Protein L-isoaspartyl methyltransferase (HuD protein family and its involvement in Small Cell Lung Carcinoma) in passive synthase reactions characterized by GC/Mass Spectrometry.
Photoelectron spectroscopy used to monitor the lab environment.
California Institute of TechnologyPasadena, CATheoretical Physics
Investigated the effects of O2 on the temperature dependent properties of a Type II YBa2Cu2O7 Superconductor.
Explored the transition from the Meissner to the vortex state.