Research Scientist with a strong immunology and stem cell background coupled with 12 years of innovative assay development skills and 4 years of GCLP lab managerial expertise looking to expand my career in science.
CTL Immunospot, Viacount and Guava Expressplus, Microsoft Office, Adobe Photoshop, Live Cycle Designer and DC.
Department of Viral Pathogenesis
Department of Hematology & Oncology
General Laboratory Management.
Overseeing general operation of laboratory and GCLP adherence.
Hiring, training and supervising junior researchers.
Coordinating laboratory duties with members in laboratory.
Troubleshooting and discussion of experimental assays with lab members.
Reporting project progress to sponsors.
Acting as a liaison between laboratory and division administration.
Developing budgets for standardized assays.
Negotiating service contract costs for laboratory equipment.
Ensure Institutional Biosafety compliance and COMS approvals
Write standard operating procedures (SOPs) for immunology assays.
Qualified in Biosafety Levels 2 culture room management.
Perform quality control for new batches of reagents.
Extensive aseptic cell culture knowledge - Primary and cell line culturing of both suspension and adherent cells.
Cell Counting - Trypan Blue exclusion with hemocytometer and Guava Easycyte Cell counter.
Cell sorting- AutoMACS and manual magnetic technique.
Preparation of cells for flow cytometry - surface, intracellular and nuclear staining.
Immune assays - T cell & B Cell ELISpot, Neutralization and Antibody Dependent Cellular Phagocytosis.
Monoclonal antibody production and purifications.
Scientific Publications- 18 PubMed indexed publications in reputed journals.
Laboratory Manager11/2013 to CurrentCenter for Virology and Vaccine research, Beth Israel Deaconess Medical Centre/Harvard Medical UniversityBoston, USA, Massachusetts
Managing a pre-clinical Non-Human Primate HIV vaccine research lab by ensuring adequately trained staff.
Planning and designing standard operating procedures (SOPs) to maximize productivity.
Collaborating with research teams nation-wide to standardize critical assays.
Assisting the PI and the Program Manager with budgeting and grant writing.
Ensuring institutional biosafety compliance as the lab biosafety officer.
Enhanced the lab portfolio by developing assays like B cell ELISpot, viral inhibition assay.
Analyzed multi-dimensional data generated from multi-faceted studies with longitudinal samples.
Recommended, developed and maintained a stream-lined record maintenance of standardized assays increasing accountability and reproducibility.
Sr. Research Associate-Assistant II11/2006 to 11/2013Beth Israel Deaconess Medical Center/Harvard Medical UniversityBoston, USA, Massachusetts
Tested efficiency of SIV/HIV vaccination regimens using ELISpot and Flow Cytometric assays on mice,
Non-Human Primates and humans.
Mononuclear cell extraction from peripheral blood and mucosal biopsy specimens for immunologic
Evaluated vaccine efficacy by cytokine characterization using intercellular cytokine staining.
Economized in-lab monoclonal antibody production cost (200 times more cost-efficient than the
comparable commercial product).
Neutralization antibody profile generation for over 900 longitudinal clinical trial samples.
B-lymphoblastoid cell line generation from primate and human blood cells for immortalized cell culture.
Scaled-up hybrid SHIV virus challenge stocks of varying infectivity for pre-clinical trials.
Quality Controlled all the above mentioned assays by inventory control and bridge testing between
Research Assistant05/2006 to 10/2006Veterans Affairs HospitalDallas, USA, Texas
Evaluated chronic myelogenous leukemia medications by suspension and adherent cell culture techniques.
Performed cell proliferation assays to understand the pharmacokinetics of treatment options.
Protein estimation and Western blotting on bone marrow stem cells to understand drug dosage effects.
Graduate Teaching and Research Assistant06/2005 to 05/2006Department of BioengineeringArlington, USA, Texas
Designed teaching aides to help students understand the concepts of Tissue Engineering.
Developed a student friendly lab module covering the basics of cell culture, scaffolding, protein coatings,
histological procedures and mammalian cell culture techniques.
In-vitro culturing and growth rate measurement of Human Mesenchymal Stem Cells.
Prepared slides by cytospin deposition and performed H&E staining
Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells.
Compared the serum-free versus serum rich media to arrive at the optimum serum concentration.
Recommended the addition of HB-EGF as a growth stimulant.
Researched the effects of cryoprotectants and freezing techniques.
Evaluated various methods to improve the viability of cells in liquid Nitrogen.
Studied the effect of cell density in the cryovial on the survival rate.
Arrived at the best possible cryopreservation technique for mammalian cell lines and stem cells
Intelligent Cane Using Mixed Signal Processing (MSP).
Conducted surveys to improve the ordinary white cane used by the visually challenged.
Eliminated bulky electronic interfaces by using an MSP chip thereby making it more affordable and
ergonomic than other smart canes.
Designed a successful collision prevention alarm system to detect obstacles.
Improved user-safety by incorporating water detection and smoke detection modules to the cane.
Education and Training
Master of Science: Biomedical EngineeringMay 2006University of TexasArlington, USABiomedical Engineering 3.22 Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells
Bachelor of Engineering: Electronics & CommunicationMay 2003Bharathiar UniversityCoimbatore, IndiaElectronics & Communication Distinction Intelligent Cane Using Mixed Signal Processing (MSP)
Adobe Photoshop, budgeting, cell culture techniques, cell culture, clinical trials, com, DC, designing, Email, grant writing, inventory control, Managing, Microsoft Office, Optimization, Quality, research, safety, surveys, teaching, vaccine, Western blotting