Leading Advancements in Probiotics & Microbiome Science Proven success driving groundbreaking advancement within probiotic applications towards microbiome-associated gastrointestinal disease through bacterial genetics & genomics. Track record of delivering: Next-generation probiotic development and application to antibiotic-associated infection prevention. Data analysis pipelines for functional genomics comparisons and big data integration. Genetic mutagenesis tools for studying beneficial properties of bacteria.
Molecular Biology (RNA/DNA isolation, cloning, gene expression, protein purification, PCR/qPCR, western blotting, ELISA), Microbiology (aerobic/anaerobic culturing and isolation, phenotyping, MICs, gram staining), Bacterial Genetics (targeted insertional mutagenesis, random transposon and transposome mutagenesis), Next Generation Sequencing Microbiome Data Analysis (UPARSE, Qiime, Mothur), Microbial Genomics (assembly/annotation software, comparative genomics software, antibiotic resistance marker detection), Unix/Linux, Windows, Vector NTI.
Available for Nationwide or International Travel.
Instructor, 01/2010 to CurrentMicrobial Genetics & Genomics － Houston, TX
Manage basic science and translational research laboratory and projects focused on developing probiotics-based microbiome manipulations for the prevention of antibiotic-associated gastrointestinal infection.
Research Project Highlights: Led research and development of patented probiotic application for anti-infectives in gastrointestinal disease.
Awarded Digestive Diseases Center Pilot & Feasibility award (2014-2015) and renewal (2015-2016) for grant entitled "Reuterin - Investigation of a novel signaling mechanism in gut-microbial health." Bioinformatics and Microbial Genomics Lead Investigator on NIH NIAID UO1 award ($7.5 million; Savidge - PI; 2016-2021) entitled, "Decoding antibiotic-induced susceptibility to Clostridium difficile infection." Contributed to launching the nation's first hospital-based microbiome center.
Evaluated microbiome data analysis pipeline integrity and improved standard analysis pipeline procedures.
Established microbial genomics data analysis pipelines for de novo genome assembly, variant calling, genomic epidemiology and antibiotic resistance marker surveillance.
Directed R&D efforts for random mutagenesis tool development in the human-derived probiotic Lactobacillus reuteri.
Participated in research project that defined the probiotic-directed conversion of dietary histidine to histamine and its relevance to decreasing intestinal inflammation.
Spinler, PhD 713) 724-8842 Page 2 Postdoctoral Research BAYLOR COLLEGE OF MEDICINE (ranked 20th in the US for biomedical research and #1 in Texas for NIH funding.
Postdoctoral Associate, 01/2006 to 01/2010Department of Pathology & Immunology
Designed, conducted and completed research on the design, development and application of mutagenesis strategies in probiotic L.
reuteri to elucidate molecular mechanisms of probiosis in gastrointestinal disease.
TEXAS CHILDREN'S MICROBIOME CENTER Texas Children's Hospital; 4th Best Children's Hospital in the US － Houston, TX
PhD:Microbiology & ImmunologyUNIVERSITY OF COLORADO HEALTH SCIENCES CENTER － Aurora, TXMicrobiology & Immunology
BS:ChemistryTEXAS STATE UNIVERSITY － San Marcos, TXChemistry
Spinler JK, Auchtung J, Brown A, et al, "Next generation probiotics targeting Clostridium difficile infection through precursor-directed antimicrobial biosynthesis," Infection & Immunity - In Review Spinler JK, Sontakke A, Hollister EB, et al, "From prediction to function using evolutionary genomics: human-specific ecotypes of Lactobacillus reuteri have diverse probiotic functions," Genome Biol Evol Vol. 6, pp. 177289, June 2014 Hemarajata P, Gao C, Pfulghoeft KJ, Thomas CM, Saulnier DM, Spinler JK, Versalovic J, "Lactobacillus reuteri-specific immunoregulatory gene rsiR modulates histamine production and immunomodulation by Lactobacilus reuteri," J Bacteriol Vol. 195, pp. 5567-76, December 2013 Santos F*, Spinler JK*, Saulnier DM*, et al, "Functional identification in Lactobacillus reuteri of a PocR-like transcription factor regulating glycerol utilization and vitamin B12 synthesis," Microb Cell Fact Vol. 10, pp. 55, July 2011 (*co-first authorship) Spinler JK, Zajdowicz SL, Haller JC, et al, "Development and use of a selectable, broad-host-range reporter transposon for identifying environmentally regulated promoters in bacteria," FEMS Microbiol Lett Vol. 291, pp.143-50, February 2009 Spinler JK, Taweechotipatr M, Rognerud CL, et al, "Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens," Anaerobe Vol. 14, pp. 166-71, June 2008 (one of the Top10 cited articles of 2008) For a complete list of publications, visit: https://www.ncbi.nlm.nih.gov/pubmed/?term=spinler+jk Technology Tools (complete list on request)
basic, conversion, data analysis, epidemiology, novel, PhD, Research