My driving force in research is to identify problems and provide practical solutions for the same. I am a multiple winning solver on Innocentive (Crowdsource initiative by former Eli Lilly scientists) and I have successful applied for internal grants at Oak Ridge National LabI am a nanoparticle engineer with interdisciplinary expertise in biomedical engineering, cancer imaging, multidrug-resistance, nanomaterials synthesis and analytical chemistry. I have work experience in an GMP setup, with more than twelve years of experience in the synthesis, modification, and characterization of nanoparticles (NPs) for multimodal biomedical imaging, targeted drug delivery, combined theragnostics, nanotoxicology screening and nanoplasmonic biosensors. I possess a broad spectrum of technical skills including but not limited to, confocal fluorescence microscopy, flow cytometry, electron microscopy (TEM & SEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), inductively coupled plasma optical emission spectrometry (ICP-OES), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), micro-CT and magnetic resonance imaging (MRI). I am skilled in handling both in vitro (eukaryotic & bacterial cell culture) and in vivo (zebrafish & mouse) model systems.I possess excellent verbal, written and visual communication skills as demonstrated by extensive publications/presentations in peer-reviewed journals and international conferences. My biggest motivation is to solve practical challenges faced in biomedical research. My experience so far has given me a diverse skillset that allows me to work strongly as an individual and in a team setting.
|Nanoparticle Synthesis • Conjugation & Functionalization of Nanomaterials • Composition & Surface Characterization Techniques • Nanotoxicity Studies • Bacterial & Mammalian Cell Culture Methods||ransmission Electron Microscopy • Scanning Electron Microscopy • Dynamic Light Scattering (Size & Shape); Fourier Transform Infrared Spectroscopy • Proton Nuclear Magnetic Resonance Spectroscopy (Surface Chemistry); X-ray diffraction • Electron Diffraction • Energy Dispersive X-Ray Spectroscopy (Crystallinity & Elemental Composition); Optical Microscopy • Fluorescence Microscopy • UV/Vis Spectroscopy (Optical Properties); Zeta Potential (Surface Charge); Superconducting Quantum Interference Device measurements (Magnetic Properties).|
Project:Developed dual mode detectable, nanoparticle based analytical probes for biomedical applications and pharmacokinetics probes. Design and development of micro-scale devices for 3D tissue culture to study and mimic biological organ systems.● Designed and executed new schemes for carbon-14 radiolabeling of nanoparticles ● Acted as a liaison between ORNL staff scientist and project managers at Battelle.
✔ 4 manuscripts published in peer reviewed journals
✔ Ensured budget surplus, by reducing the number of comparative biodistribution experiments, by engineering magnetic nanoparticles and silica nanoparticles to have chemically identical radiolabeled surfaces
✔ 2 successful Grant Applications:Center for Nanophase and Materials Sciences (CNMS) at ORNLa. CNMS2013-153 (2013 - present)b. CNMS2015-R06 (2014 - 2015)
✔ Top 0.2% of InnoCentive users who have provided a winning solution. (https://www.innocentive.com/ar/solver/show/325814)InnoCentive has more than 355,000 registered users internationally with over 68.5% of these users being Ph.D. degree holders. InnoCentive is a crowd source initiative to find solutions to practical problems faced in an industrial or research lab setting. This initiative is synergistic with my interest in applying my knowledge to provide practical solutions for real-world research problems. The challenges require only theoretical solutions and are therefore allowed under my current employer's conflict of interest policy.
Winning solution: ● ID9933675 - Incorporating Drugs into an Exosome
✔ 1 manuscripts published in peer reviewed journals; 1 manuscript in review; 2 manuscripts in preparationProject:Synthesis of targeted, bio-functionalized nanoparticle contrast agents for spectral color X-ray imaging and magnetic resonant imaging (MRI) for use in in vivo model systems and in vitro X-ray phantoms.● Size and shape controlled synthesis of gold, hafnium oxide and gadolinium oxide nanoparticles● Core-shell nanoparticles with gold or gadolinium oxide or hafnium oxide cores and silica shells● Gold nanorods● Gadolinium oxide nanoplates● Fluorescent nanoparticles● T1 and T2 MRI contrast agentsIn Vivo model systems:● Zebrafish (Danio rerio) ● Mice (BALB/c; Non Obese Diabetic)Teaching experience:● Mentored summer undergraduate research fellows who are NDnano Undergraduate Research Fellows (NURF)
Pfizer Young Investigator Poster Award
5th Annual Harper Research Day Poster Competition
● Shape controlled synthesis of nanomaterials.● Screening for anti-cancer potential of nanoparticles.● Synthesis of proteoliposomes for studying multi-drug transporter proteins.Nanoparticles● Standardized large volume aqueous phase synthesis of nanomaterials.● Surface modification of nanomaterials● Bio-functionalization of nanomaterials● Bio-compatibility/Toxicology of nanomaterials● Non photobleaching nanoparticles as photonic probes to analyze multidrug resistance pumps in bacteria and in ovarian cancer cellsImaging● Optical (Dark field/ phase contrast) and epi-fluorescence microscopy and spectroscopy● Transmission electron microscopy/ Scanning electron microscopy/ EDXS● Sub-diffraction imaging using nanoparticles as optical probesCell Culture and Cell Biology● Bacterial culture and handling● Eukaryotic adherent/suspension cell culture and handling● Histology/TEM biological sample preparation and sectioning● Basic molecular biology & cell biology lab techniquesTeaching Experience:● Graduate teaching assistant for general chemistry lab (CHEM101).
✔ 10 manuscripts published in peer reviewed journals✔ doi:10.1021/nn700048y: Made the list of Most-Accessed Articles of 2007; Featured in Chemical and Engineering News✔ doi:10.1039/c0nr00080a: Highlighted on the back cover of that issue of RSC Nanoscale✔ 14 publications to date from my graduate lab, as a result of my novel technique of synthesizing thiol-free silver nanoparticles, stable in physiological media
Materials Research Society
American Chemical Society
Materials Science & Technology
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