Experienced, well published, T cell immunologist seeking to join an enthusiastic research team, to assist in the development of novel therapeutic molecules and ultimately to cure disease. Areas of expertise includes Molecular Biology, cell Biology and Immunology *By nature self-motivated, creative thinker and fast learner *Highly motivated and talented in experiment design
Over 20 years of scientific research experience (Immunology, cell & molecular biology)Disease expertise : Allergic, autoimmune diseases and Immune-oncologyExpert in immune cell Biology (activation, differentiation, regulation-mRNA & protein profiling, sorting and phenotyping assay) as well as tissue resident cellsLead and participated in new target identification and validation teamSkill in various technologies : mRNA (Nanostring and Nextera : multicell and single cell), DNA (CRISPR/CAS9), FACS (Analyser and sorter), Imaging (Fluorescence and Confocal), Multiplex (Luminex: cytokines and chemokines), MSD & Array and many immune profiling assaysDesigning and performing experiments for new targets and PD/predictive/prognostic biomarkers for programs in Lead Identification, Optimization and Preclinical CandidatesStrong background in Cellular and Molecular BiologyLead many academic and industry collaborations : 3D culture, human disease tissuesExcellent communication and presentation skills Extensive understanding on the disease mechanisms of asthma, autoimmune and cancerSolid experience in pediatric immune responses Multiple animal handling abilities Creative and collaborative approach to reach a goalParticipated in many mentor and mentee programs
| Immunology T cell biology (TCR and cytokine mediated T cell activation and differentiation, regulatory T cell and DCs) : Sorting and profiling during activation and regulationIn vitro and in vivo ligand receptor interactions and activation (proliferation, cytokines and chemokines, gene expression profiling)Disease expertise in asthma, autoimmunity and Immune-oncologyAntibody based therapeutic studies in miceImmunohistochemistry using frozen and paraffin embedded tissue samplesMulti-color FACS flow technique (FACS Canto, Fortessa and Sony SH800 sorting)ELISA and ELISPOT technique : R&D, MSD, EbiosciencesParticipated in Knock-out mouse designTetramer based immunostainingAsthma related studies measuring Airway hyperreactivity and inflammationDifficult mouse injection skills: facial vein (pups), intra vein, intra peritoneal , intra nasal and intra tracheal injectionsMouse bleeding techniques: retro-orbital, tail, total bleeding. | Molecular Biology Signaling pathway mRNA based arrays and phosphospecific antibodyWestern Southern and Northern Blot technique for genotypingAntibody production using proteins and peptidesMass production and purification of monoclonal antibody using rollersReal time PCR : many array systemsProduction and purification of recombinant proteinCell Biology Pathway of cell viability and apoptosis in the immune systemLive and fixed cell imaging techniques using confocal and fluorescence microscopy Various apoptosis test: (activated caspase detection, membrane integrity, DNA degradation and mitochondrial apoptotic pathways)Cell purification using the Auto MACS systemMultiplex Luminex systems |
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