University of California, San Diego-University of Alabama O'Brien Center NIDDK Rodent Kidney Injury Skills Course, 2014.
San Diego, CA.
Volker Vallon, Dr.
American Physiological Society Professional Skill Training Course: Writing and Reviewing for Scientific Journals, 2014.
David Pollock (UAB).
American Physiological Society Professional Skills Training Online Course: Writing and Reviewing for Scientific Journals, 2013.
Assistant research scientist specializing in cell culture development with a strong background in molecular cell biology. Proficient in mammalian cell culture, fed-batch culture and perfusion cell culture. Highly organized, with well-developed communication, a strong team spirit and time management skills.
Knowledge of GMP and GLP regulations, mammalian and bacterial cell culture, 2L-15L bioreactors, advanced microscale bioreactor system (ambr), blood gas analyzer, YSI 2700/2950, osmometer, cedex cell counter, Sartorius DCU, SDS-PAGE (Western blot, dot blot, northern blot), tissue DNA/protein extraction, qPCR, cloning, plasmid preparation, UV spectrophotometer (ELISA, EIA, colorimetric assays), and histology.
Computer Programs: Fermworks, Microsoft office (intermediate; excel/developer, powerpoint, word), Systat 13, Statview, Prism Graphpad, R statistical software, ImageJ, ImageStudio, EndNote
12/2014 to Current
Assistant Research ScientistCell Culture Development, Bayer Healthcare
Analytical instrument maintenance in compliance with GMP and GLP specifications.
Developed training modules from SOP specifications.
Trained in GLP and GMP aseptic technique as well as scale-up cell culture.
Experience with fed-batch cell culture (ambr microbioreactor, 2L and 5L) and perfusion cell culture (15L).
05/2014 to 08/2014
NIH MHIRT Research FellowKagawa Medical University
Project leader; conducted a renal ischemic reperfusion study on insulin resistant rats using survival surgery.
Mentored three undergraduate students in the biomedical research field.
Taught animal handling, surgical, and laboratory techniques.
08/2011 to 05/2014
Graduate Student ResearcherUniversity of California
Conducted experiments in order to complete thesis work.
Worked independently as well as mentoring several undergraduate students in laboratory techniques.
Analyzed the outcome of experiments and interpreted data in order to publish manuscript.
06/2010 to 07/2011
Research Assistant/Lab ManagerUniversity of California
Conducted experiments for the completion of projects for graduate and post-doctoral students.
Mentored undergraduate students in laboratory techniques and animal handling.
Other tasks include
04/2009 to 05/2010
Undergraduate Research AssistantUniversity of California
Assisted graduate students in experiments for manuscript publication and thesis completion.
Master's of Science: Quantitative and Systems Biology University of California -
Bachelor's of Science: Biological Sciences Human Health Psychology University of California - Merced Biological Sciences 3.031/4.0 Human Health Psychology
NIH Minority Health and Health Disparities International Research Training (MHIRT) Program Kagawa University Faculty of Medicine, Kagawa Prefecture, Japan. 2014, 2010-2011*UC Merced Quantitative and Systems Biology Graduate Student Summer Fellowship, University of California, Merced: uc merced -
*USDA Hispanic Minorities Fellowship: University of California - Merced Francis M. Benton Scholarship
Angiotensin Receptor Blockade Increases Pancreatic Insulin Secretion and Decreases Glucose Intolerance during Glucose Supplementation in a Model of Metabolic Syndrome. Rubén Rodriguez, Jose Viscarra, Jacqueline N. Minas, Daisuke Nakano, Akira Nishiyama and Rudy M. Ortiz. Journal of Endocrinology. April 2013
*Considerations when quantitating protein abundance by immunoblot. Alicia A. McDonough, Luciana C. Veiras, Jacqueline N. Minas and Donna Lee Ralph. American Journal of Physiology - Cell Physiology. December 2014
MANUSCRIPTS IN PREPARATION
*High Fat Consumption Exacerbates and Angiotensin Receptor Blockade Alleviates Impaired Glucose Tolerance and Antinatriuresis in a Model of Metabolic Syndrome. Jacqueline N. Minas, Shaina Balayan, Daisuke Nakano, Mouhamed S. Awayda, Akira Nishiyama, and Rudy M. Ortiz
*Angiotensin and Mineralocorticoid Receptor Antagonism Attenuates Cardiac Oxidative Stress in Angiotensin II-Infused Rats. Jacqueline N. Minas, Max A. Thorwald, Debra Conte, Jose-Pablo Vázquez-Medina, Akira Nishiyama, and Rudy M. Ortiz
*Angiotensin II and mineralocorticoid receptor antagonism decrease cardiac nitrotyrosine content and recover glutathione peroxidase activity in Angiotensin II-infused rats. Jacqueline N. Minas, Max A. Thorwald, Debra Conte, Jose-Pablo Vázquez-Medina, Akira Nishiyama, and Rudy M. Ortiz. Experimental Biology Conference, San Diego, CA April 2014
*High fat diet decreases fractional sodium excretion and increases systolic blood pressure via Angiotensin II-mediated increase in epithelial sodium channel abundance. Jacqueline N. Minas, Daisuke Nakano, Mouhamed S. Awayda, Akira Nishiyama, and Rudy M. Ortiz. Experimental Biology Conference, San Diego, CA. April 2014
*High Fat Diet Contributes to Improper Kidney AT1 Activation and Impaired Sodium Regulation through ENaC Cleavage in a Model of Insulin Resistance. Jacqueline N. Minas, Ruben Rodriguez, Mouhamed S. Awayda, and Rudy M. Ortiz. Experimental Biology Conference, Boston, MA. April 2013
Master's of Science : Quantitative and Systems Biology Bachelor's of Science : Biological Sciences Human Health Psychology NIH Minority Health and Health Disparities International Research Training (MHIRT) Program Kagawa University Faculty of Medicine, Kagawa Prefecture, Japan. 2014, 2010-2011*UC Merced Quantitative and Systems Biology Graduate Student Summer Fellowship, University of California, Merced : *USDA Hispanic Minorities Fellowship :
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