|Cell culture: Expertise in primary astrocyte and neurons cultures from embryonic and adult mice and rats as well as variety of cell lines (Hek 293, Cos, breast cancer MCF). Also have experience with astrocyte- neuron co-cultures (contact & non-contact models) Immuno-staining techniques: Expertise in different methods of immuno- staining perfused or frozen brain sections and cells in culture. Fluorescence or biotinylated tagged single and double labeling of brain tissue. Histology: Expertise in gross and fine brain tissue dissection to harvest specific brain regions. Proficient in use of cryostat as well as vibrotome. Biochemistry and molecular biology techniques: Expertise in western blotting, PCR, as well as DNA-RNA isolation from cells, and different tissues. Animal husbandry: Proficient in rodent handling, breeding, genotyping.|
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