An organized biochemist with 9 years of experience in laboratory research, 8 peer reviewed publications
Specialized in Polyketide synthase chemistry in Azinomycin biosynthesis, genetic engineer of Streptomyces, Construction and screening of genomic library, mutagenesis, large scale protein expression, Fermentation, biotransformation, enzymatic activity assays, protein engineering and protein purification
Successful in writing grants, proposals and manuscripts
Efficient in planning and management of projects
Summary of Skills
Leader in data presentation
DNA isolation expert
Ph.D: Biochemistry1 2010Institute of Biomolecule Reconstruction, SunMoon UniversitySouth KoreaBiochemistry
M.S: BotanyTribhuvan UniversityNepalBotany
Advisor Professor Tadhg P. Begley
Co-factor catabolism Isolation, Screening and identification of microorganism responsible for heme catabolism Construction of genomic library and isolation of gene cluster responsible for catabolize heme Worked as Lab manager for biosafety of laboratory Working as a team leader in planning and management of multiple projects and to identify issues and find resolution Supervised and troubleshoote graduate/undergraduate students in their research projects and worked closely with colleagues.
Postdoctoral Research Associate01/2012 to CurrentTexas A&M UniversityCollege Station, Texas
Gene manipulation/Mutagenesis Strain development Specialty in Streptomyces, e.
coli & yeast fermentation Protein biochemistry Protein purification Specialty in enzyme activity assay Expert in Analytical chemistry: HPLC, LC/MS, ESI and UV-Vis spectroscopy Supervised and trained interdisciplinary groups of people and worked collaboratively to attain specific goals Presented and explained diverse scientific topics Experimental Design ACCOMPLISHMENTS Advance research was accompanied in strain engineering to explore and prove Thioesterase Domain AziG which controls Polyketide Synthase Chemistry in Azinomycin Biosynthesis and Unraveling the Biosynthetic Intricacies of Azabicycle Formation in the DNA Crosslinking Agent Azinomycin B Activation of cryptic metabolites by genetically engineered Streptomyces sahachiroi.
Completed research on Streptomyces sp.
KCTC 0041BP for the production of novel xylosyl-attached PKS type I hybrid compound 5-O-xylosyl-chalconolide derivative.
Metabolically engineered E.
coli for the successful production of anti-oxidant drug (glycosylated-flavonoid).
Postdoctoral Research Associate01/2010 to 01/2012Texas A & M University
Advisor: Professor C.M.H.
Watanabe Research: Unraveling of Azinomycin Biosynthesis-An antitumor compound Evaluating innovative techniques like: established Streptomyces sahachiroi knock-out system for the characterization of antitumor compound Azinomycin B, developed E.
coli as a plat form for the heterologous expression of azinomycin biosynthesis genes.
Working as a team leader in planning and management of multiple projects and to identify issues and find resolution Routinely operated fermenter (10L) to carry out azinomycin and its analogues production using Streptomyces.
Research Assistant01/2005 to 01/2010Institute of Biomolecule Reconstruction, SunMoon University
Genetic engineering of microorganisms Genomic library construction, Perform gene knockout experiment to prove the function and metabolic engineering of microorganism (Streptomyces and E.
coli) for the production of glycosylated natural products (Macrolide and antioxidants) Strain isolation, characterization and natural product isolation and analysis by HPLC, LC-MS, NMR techniques Established the genetically engineering approach for the production of xylosylated, rhamnosylated and allosylated flavonoids Designed and conducted research using molecular biology and chemistry skills Managed laboratory functions including organization, ordering and scheduling equipment use Trained and mentored junior lab members until they can work independently.
Simkhada, D.; Zhang, H.; Mori, S.; Sikowitz, M.; Ealick, S.; Watanabe, C. M. H. "Thioesterase Domain AziG Controls Polyketide Synthase Chemistry in Azinomycin Biosynthesis," 2014, manuscript in preparation. Simkhada, D.; Sharma, V.; Kelly, G. T.; Zhang, H.; Yu, F.; Watanabe, C. M. H. "Unraveling the Biosynthetic Intricacies of Azabicycle Formation in the DNA Crosslinking Agent Azinomycin B," 2014, manuscript in preparation. Selected Publication Simkhada, D.; Zhang, H.; Mori, S.; Williams, H.; Watanabe, C. M. H. "Activation of cryptic metabolite production through gene disruption: Dimethyl furan-2,4-dicarboxylate produced by Streptomyces sahachiroi," Beilstein J Org Chem. 2013; 9:1768-73. Pandey, R.P.; Malla, S.; Simkhada, D.; Kim. B.G.; Sohng, J.K. "Production of 3-O-xylosyl quercetin in Escherichia coli." App. Microbial. Biotechnol, 2013 Mar; 97(5) : 1889-901 Simkhada, D.; Lee, HC.; Sohng, J.K. "Genetic engineering approach for the production of rhamnosyl and allosyl flavonoids from Escherichia coli." Biotechnol Bioeng. 2010, 7(1): 154-162. Simkhada, D.; Oh T.J.; Pageni, B.B.; Lee, H. C.; Liou, K.K.; Sohng, J. K.; "Characterization of CalS9 in the biosynthesis of UDP-xylose and the production of xylosyl-attached hybrid compound." App. Microbial. Biotechnol, 2009, 83:885-895. Link to the complete list of my publications, poster and presentations: http://scholar.google.com/citations?user=LSmp-3EAAAAJ http://www.ncbi.nlm.nih.gov/pubmed/?term=Dinesh+simkhada
approach, C, oral communication, excellent communication, HPLC, Innovation, team leader, natural, NMR, novel, Problem analysis, problem solving skills, reading, Research, scheduling, scientific, Team-Player, type I, UV, written, writing skills